bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: April 12, 2023
Abstract
Trichoderma
harzianum
is
a
filamentous
ascomycete
frequently
applied
as
biocontrol
agent
in
agriculture.
While
mycoparasitism
and
antagonism
of
spp.
against
fungal
pathogens
are
well
known,
early
responses
to
the
presence
plant
await
broader
investigation.
Analyzing
stages
plant-fungus
communication
we
show
that
T.
B97
chemotropically
responds
extract
both
fungus
alter
secondary
metabolite
secretion
upon
recognition.
We
developed
strategy
for
omics-analysis
simulating
conditions
recognition
eliciting
chemotropic
response
found
102
genes
be
differentially
regulated,
including
nitrate
nitrite
reductases.
Additionally,
previously
uncharacterized
P
lant
C
ommunication
A
ssociated
(PCA)
gene
cluster
was
strongly
induced
plant,
comprises
palindromic
DNA
motif
essential
colonization.
The
PCA-cluster
only
present
Harzianum
clade
closely
related
homologous
Metarhizium
Horizontal
transfer
(HGT)
detected
by
plants,
while
likely
under
balancing
or
positive
selection.
Hence,
mediates
fungus-plant
chemical
may
responsible
high
potential
species
applications.
Plain
language
summary
Interactions
plants
with
fungi
–
beneficial
pathogenic
crucial
ecological
function
partners.
Yet,
“language”
they
use
how
when
it
still
insufficiently
known.
describe
discovery
novel
cluster,
which
plant-fungal
interkingdom
interaction
biocontrol-agent
.
Biological Control,
Journal Year:
2024,
Volume and Issue:
192, P. 105479 - 105479
Published: March 5, 2024
Demethylase
inhibitors
(DMIs)
targeting
CYP51
proteins
in
fungi
are
often
used
for
plant
disease
management.
Trichoderma
harzianum,
a
widely
biocontrol
agent,
however,
is
sensitive
to
DMIs.
This
negates
their
combined
use
as
strategy
practices.
Increasing
the
tolerance
of
T.
harzianum
DMIs
represents
feasible
solution
this
problem.
In
regard,
overexpression
CYP51B
variant
Mycosphaerella
graminicola
(MgCYP51B-v)
conveys
increased
Molecular
docking
indicated
distinct
decrease
affinity
MgCYP51B-v
with
Since
conserved
M.
and
we
attempted
increase
DMI
by
heterologously
expressing
MgCYP51B-v.
A
series
mutants
were
obtained
that
able
develop
colonies
on
tebuconazole-amended
medium.
Two
representative
selected
further
studies
confirmed
they
resistant
tebuconazole
resistance
factors
(RFs)
approximately
10.0.
The
maintained
similar
level
after
ten
rounds
subculture
without
fungicide,
indicating
fungicide
was
stably
inherited.
also
exhibited
other
DMIs,
including
metconazole,
ipconazole,
hexaconazole,
prochloraz.
Further
assessment
did
not
have
any
impact
fitness.
improved
compatibility
between
enables
utilization
which
these
control
agents
together.
Such
has
potential
provide
synergistic
prevent
or
delay
development
resistance.
Pest Management Science,
Journal Year:
2023,
Volume and Issue:
80(3), P. 1153 - 1167
Published: Oct. 24, 2023
Diseases
caused
by
Fusarium
pathogens
lead
to
significant
yield
losses
on
many
economically
important
crops.
The
purpose
of
this
study
was
evaluate
the
antagonistic
capability
and
chemical
profile
bioagent
Trichoderma
asperellum
against
several
strains.
efficacy
strain
in
reducing
Fusarium-root
rot
disease
dry
bean
also
examined.
Journal of Fungi,
Journal Year:
2024,
Volume and Issue:
10(8), P. 547 - 547
Published: Aug. 3, 2024
spp.
are
filamentous
fungi
generally
observed
in
nature,
which
widely
marketed
as
biocontrol
agents.
The
secondary
metabolites
produced
have
obtained
special
attention
since
they
possess
attractive
chemical
structures
with
a
broad
spectrum
of
biological
activities.
In
Cuba,
the
species
Journal of Toxicology and Environmental Health Part B,
Journal Year:
2023,
Volume and Issue:
26(2), P. 97 - 126
Published: Feb. 6, 2023
Trichoderma
is
a
saprophytic
fungus
that
used
worldwide
as
biocontrol
and
biofertilizer
agent.
Although
considered
nonpathogenic
until
recently,
reports
of
human
infections
produced
by
members
the
genus
are
increasing.
Numerous
sources
infection
were
proposed
based
upon
patient
data
phylogenetic
analysis,
including
air,
agriculture,
healthcare
facilities,
but
deficit
knowledge
concerning
makes
treatment
difficult.
These
issues
compounded
isolates
present
profiles
which
exhibit
high
minimum
inhibitory
concentration
values
to
available
antifungal
drugs.
The
aim
this
review
global
distribution
affect
both
immunocompetent
immunocompromised
hosts,
clinical
features,
therapeutic
strategies
treat
patients,
well
highlighting
treatments
with
best
responses.
In
addition,
susceptibility
have
emerged
in
recent
decades
examined
drugs
need
be
further
evaluated
potential
candidates
also
indicated.
Diversity,
Journal Year:
2024,
Volume and Issue:
16(1), P. 68 - 68
Published: Jan. 20, 2024
Trichoderma
is
a
genus
of
cosmopolitan
fungi
with
more
than
375
species
described
today.
Despite
its
global
significance
in
agriculture,
ecosystems,
and
industry,
few
studies
have
focused
on
studying
the
diversity
distribution
this
Mexico.
In
systematic
review
meta-analysis,
we
aimed
to
understand
Mexico,
both
ecosystems
agroecosystems.
For
review,
used
PRISMA
methodology.
We
reviewed
forty-one
scientific
articles,
two
book
chapters,
GBIF
database.
recorded
total
1082
isolates,
revealing
presence
57
29
states
found
that
from
were
reported
20
agroecosystems
6
ecosystems.
T.
harzianum
was
predominant
agricultural
undisturbed
soil.
Tabasco
Veracruz
highest
diversity,
14
reported,
respectively.
Chiapas
had
indices
(Menhinick
3.20,
Simpson
0.89,
Margalef
4.16).
The
coffee
crop
agroecosystem
12
reported.
tropical
rainforests
featured
different
species.
This
study
highlights
as
genus.
argue
for
importance
comprise
applications
social
benefits.
Acta Biochimica et Biophysica Sinica,
Journal Year:
2024,
Volume and Issue:
56(6), P. 825 - 832
Published: April 29, 2024
Biological
control
of
pests
and
pathogens
has
attracted
much
attention
due
to
its
green,
safe
effective
characteristics.
However,
it
faces
the
dilemma
insignificant
effects
in
large-scale
applications.
Therefore,
an
in-depth
exploration
metabolic
potential
biocontrol
fungi
based
on
big
omics
data
is
crucial
for
a
comprehensive
systematic
understanding
specific
modes
action
operated
by
various
fungi.
This
article
analyzes
preferences
extracellular
carbon
nitrogen
source
degradation,
secondary
metabolites
(nonribosomal
peptides,
polyketide
synthases)
their
product
characteristics
conversion
relationship
between
primary
metabolism
intracellular
eight
different
filamentous
with
appropriate
biological
bacterial
phytopathogenic
nematodes.
Further
clarification
provided
that
Paecilomyces
lilacinus,
encoding
large
number
hydrolase
enzymes
capable
degrading
pathogen
protection
barrier,
can
be
directly
applied
field
as
predatory
fungus,
whereas
Trichoderma,
antibiosis-active
form
dominant
strains
preferred
substrates
produce
achieve
antibacterial
effects.
By
clarifying
levels
achievable
fungi,
we
provide
theoretical
foundation
application
cropping
habitats.
