Beyond Restoration: Coral Microbiome Biotechnology

Coral reefs of the world, Journal Year: 2025, Volume and Issue: unknown, P. 215 - 234

Published: Jan. 1, 2025

Language: Английский

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Stronger together: harnessing natural algal communities as potential probiotics for inhibition of aquaculture pathogens

Microbiology Spectrum, Journal Year: 2025, Volume and Issue: unknown

Published: May 21, 2025

ABSTRACT Intensive fish rearing in aquaculture is challenged by infectious diseases, and although vaccines have been successfully developed for mature fish, alternative disease control measures are needed larvae juveniles. Probiotics offer a promising to antibiotics, with the potential reduce risk of antibiotic resistance. typically isolated used as pure cultures; however, natural environments, it concerted effort complex microbiome that keeps pathogens at bay. Here, we an vitro assay evaluate anti-pathogen efficacy mixed algal microbiomes from live feed microalgae Tetraselmis suecica Isochrysis galbana . The inhibition green fluorescent protein (GFP)-tagged Vibrio anguillarum , key pathogen, microbial communities was measured quantified reduction fluorescence. more inhibitory V. than microbiome. During co-culture bacterial density increased, while diversity reduced determined metataxonomic analyses. Bacteria fully were members Alteromonadaceae, Halomonadaceae, Rhodobacteraceae, Vibrionaceae, Flavobacteriaceae, Erythrobacteraceae Although some strains individually inhibited these not microbiome, enhanced observed when Sulfitobacter pontiacus D3 Vreelandella alkaliphila D2 co-cultured, even though neither monocultures. Thus, this study demonstrates derived can effects, co-cultures may synergistic advantages over monocultures probiotics, highlighting their promise health strategies. IMPORTANCE Aquaculture fastest-growing food protein-producing sector, sustainable required. gained interest solution combating pathogens, using mixtures microorganisms rather cultures represent stable pathogen control. We (GFP) tagging enabling quantitative assessment effects microbiomes. show efficiency suppression be increased compared monocultures, thus emphasizing bacteria probiotics.

Language: Английский

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Testacosides A–D, glycoglycerolipids produced by Microbacterium testaceum isolated from Tedania brasiliensis

Applied Microbiology and Biotechnology, Journal Year: 2024, Volume and Issue: 108(1)

Published: Jan. 12, 2024

Marine bacteria living in association with marine sponges have proven to be a reliable source of biologically active secondary metabolites. However, no studies yet reported natural products from Microbacterium testaceum spp. We herein report the isolation M. strain sponge Tedania brasiliensis. Molecular networking analysis bioactive pre-fractionated extracts culture media enabled discovery testacosides A-D. Analysis spectroscopic data and chemical derivatizations allowed identification A-D as glycoglycerolipids bearing 1-[α-glucopyranosyl-(1 → 3)-(α-mannopyranosyl)]-glycerol moiety connected 12-methyltetradecanoic acid for testacoside A (1), 14-methylpentadecanoic B (2), 14-methylhexadecanoic C (3) D (4). The absolute configuration monosaccharide residues was determined by

Language: Английский

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Elucidation of synbiotic diet comprising of Lactobacillus plantarum L20 and Sargassum polycystum on gastrointestinal microbiota, tissue structures and AHPND associated dysbiosis susceptibility in black tiger shrimp (Penaeus monodon)

Aquaculture, Journal Year: 2024, Volume and Issue: 594, P. 741339 - 741339

Published: July 11, 2024

Language: Английский

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Comparative Genomic Analysis of Cold-Water Coral-Derived Sulfitobacter faviae: Insights into Their Habitat Adaptation and Metabolism

Marine Drugs, Journal Year: 2023, Volume and Issue: 21(5), P. 309 - 309

Published: May 19, 2023

Sulfitobacter is one of the major sulfite-oxidizing alphaproteobacterial groups and often associated with marine algae corals. Their association eukaryotic host cell may have important ecological contexts due to their complex lifestyle metabolism. However, role in cold-water corals remains largely unexplored. In this study, we explored metabolism mobile genetic elements (MGEs) two closely related faviae strains isolated from black at a depth ~1000 m by comparative genomic analysis. The shared high sequence similarity chromosomes, including megaplasmids prophages, while both contained several distinct MGEs, prophages megaplasmids. Additionally, toxin-antitoxin systems other types antiphage were also identified strains, potentially helping overcome threat diverse lytic phages. Furthermore, similar secondary metabolite biosynthetic gene clusters genes involved dimethylsulfoniopropionate (DMSP) degradation pathways. Our results provide insight into adaptive strategy thrive niches such as level.

Language: Английский

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Heterologously expressed SacP23, a novel bacteriocin from Paenibacillus polymyxa #23, is active against methicillin resistant Staphylococcus aureus

Royal Society Open Science, Journal Year: 2023, Volume and Issue: 10(12)

Published: Dec. 1, 2023

Antimicrobial peptides have the potential to be used in a range of applications, including as an alternative conventional antibiotics for treatment bacterial infections humans and animals. Therefore, there is interest identifying novel bacteriocins which desirable physico-chemical properties or antimicrobial activities. Paenibacillus polymyxa #23, isolated from marine sponge, has wide spectrum activity against Gram-negative Gram-positive bacteria. To explore basis this activity, complete genome sequence strain was examined. Multiple genes predicted encode were identified. One gene sactipeptide bacteriocin, named SacP23. confirm that SacP23 does peptide it heterologously expressed Bacillus subtilis. A cluster eight genes, encoding full complement accessory well structural native promoter, cloned into B. subtilis BS54A. The recombinant displayed several bacteria, multi-drug resistant Staphylococcus aureus. Heterologous expression whole offers powerful way interrogate resolve various activities by strains multiple compounds interest.

Language: Английский

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Genome-resolved diversity and biosynthetic potential of the coral reef microbiome

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 18, 2024

Coral reefs are marine biodiversity hotspots that provide a wide range of ecosystem services. They also reservoirs bioactive compounds, many which produced by microbial symbionts associated with reef invertebrate hosts. However, for the keystone species coral reefs, reef-building corals themselves, we still lack systematic assessment their microbially encoded biosynthetic potential, and thus molecular resources may be at stake due to alarming decline in cover. Here, analysed genomes reconstructed from 820 samples three representative genera collected 99 across 32 islands during two-year expedition throughout Pacific Ocean (Tara Pacific). By contextualising our analyses microbiomes other species, found genomic information was previously available only 10% 4,224 overall less than 1% 645 exclusively identified Tara samples. We host-specific potential rival or even surpass traditional targets natural product discovery, such as sponges soft corals. Fire were not particularly diverse gene clusters (BGCs), but BGC-rich bacteria, including Acidobacteriota spp., have been recently highlighted promising repertoire. Together, this study unveils new candidate sources compound prioritises isolation, underscores importance conservation efforts linking macro-organismal loss biotechnological potential.

Language: Английский

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Exploring the microbial community and biotechnological potential of the sponge Xestospongia sp. from an anchialine cave in the Yucatán Peninsula

Ciencias Marinas, Journal Year: 2024, Volume and Issue: 50

Published: July 3, 2024

Sponge-associated microorganisms are key influencers of nutrient biogeochemistry and important sources bioactive natural products. This study provides the first insights into taxonomic diversity microbial community associated with sponge Xestospongia sp. from an anchialine cave in coastal area underground river Yucatán Peninsula Xcalak, Quintana Roo, Mexico, potential antimicrobial activity its cultivable bacteria. High abundances Sulfurospirillum Desulfovibrio were detected 16S rRNA amplicons, suggesting that plays role geochemical sulfur cycle. Analysis crude extracts Nocardiopsis dasonvillei NCA-454 revealed against methicillin-sensitive Staphylococcus aureus ATCC 25913 (MSSA) methicillin-​resistant ​S. 43300 (MRSA). Studies microbiomes sponges system can help elucidate biogeochemical cycles these poorly studied environments. Moreover, communities represent untapped source secondary metabolites biotechnological potential.

Language: Английский

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Antimicrobial activity of coral‐associated beneficial bacteria against coral disease‐causing microbial pathogens

Journal of Basic Microbiology, Journal Year: 2023, Volume and Issue: 64(1), P. 81 - 93

Published: Sept. 19, 2023

Abstract Microbial infection of immune‐compromised corals influences disease severity, resulting in coral mortality. However, coral‐associated beneficial bacteria are known to produce antimicrobial compounds that prevent the growth potential pathogens and invading microbes. Hence, associated with Porites lutea were isolated protein bioactive secondary metabolites extracted tested for their activity against putative prokaryotic eukaryotic pathogens. Bioactive exhibited remarkable antagonism various such as Serratia marcescens , Vibrio species, Aspergillus sydowii . Besides, Cobetia marina amphilecti Pseudoalteromonas neustonica Virgibacillus halodenitrificans manifested notable inhibition protozoan ciliates ( Uronema marinum Holosticha diademata Cohnilembus verminus Euplotes vannus ) zooplankton be involved pathogenesis diseased lesion progression. Thus, present study may benefit understanding antagonistic interactions microbial pathogens, well involvement reducing severity.

Language: Английский

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Potential probiotic aquaculture microbiomes: detecting inhibition of the fish pathogen Vibrio anguillarum by a complex algal community

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 30, 2024

Intensive fish rearing in aquaculture is challenged by infectious diseases. Although vaccines have been successfully developed for mature fish, alternative disease control measures are needed larvae and juveniles, whose immune systems not yet fully developed. Probiotics, typically isolated as pure cultures from microbiomes, offer a promising to antibiotics, with the potential reduce antibiotic use and, consequently, risk of resistance. In this study, we an vitro assay evaluate anti-pathogen efficacy only individual bacterial but also mixed algal microbiomes derived live-feed microalgae Tetraselmis suecica Isochrysis galbana, based on premise that natural inherently evolved combat pathogens. This was used compete GFP-tagged Vibrio anguillarum, key pathogen, against different microbial communities, allowing us quantify inhibitory activity through fluorescence. We found galbana microbiome more V. anguillarum than microbiome. During co-culture increased density reduced diversity determined metataxonomic analyses. The enriched could inhibit pathogen. Bacteria these were members Alteromonadaceae, Halomonadaceae, Rhodobacteraceae, Vibrionaceae, Flavobacteriaceae, Erythrobacteraceae. some strains individually inhibited enhanced inhibition observed when Sulfitobacter pontiacus D3 Halomonas campaniensis D2 co-cultured, even though neither monocultures. Thus, study demonstrates communities can effects, suggesting their probiotics. Furthermore, results indicate co-cultures may synergistic advantages over monocultures, highlighting promise health strategies.

Language: Английский

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