Exploring Uncharted Marine Bacteria as a Source of Novel Antimicrobials DOI Open Access

P Rashmi

International Journal of Oceanography & Aquaculture, Journal Year: 2023, Volume and Issue: 7(4), P. 1 - 4

Published: Jan. 1, 2023

Marine microbes are defined by their habitat as microorganisms living in the sea or ocean. The term “marine microbes” includes viruses, protozoa and fungi. This microbial life constitutes 70% To 90% of marine biomass. More than 15000 bioactive compounds have been isolated from fungi, other organisms used anti-inflammatory, anticancer leads. Among marine, bacteria least explored ones. actinobacteria adapted to high hydrostatic pressure, concentration Sodium Chloride, low organic matter, temperature. phylum is a Gram-positive, nonmotile, aerobic bacteria. Streptomyces Actinomycetes studied largely this due ability produce wide array extracellular enzymes secondary metabolites. Examples active include Fijimycins, etamycin A, Chlorinated bisindole pyrroles, dynamics A–E. There may be diverse extremo hidden shadows that currently unknown and, if discovered, open up interesting new methods for production use novel antimicrobials. In pharmaceutical research, very small fraction such studied. If discovered ingenious, cost-effective many anti-microbials.

Language: Английский

Novel [1,3,4]Thiadiazole[3,2-a]pyrimidin-5-ones as Promising Biofilm Dispersal Agents against Relevant Gram-Positive and Gram-Negative Pathogens DOI Creative Commons
Daniela Carbone, Camilla Pecoraro,

Fabio Scianò

et al.

Marine Drugs, Journal Year: 2024, Volume and Issue: 22(3), P. 133 - 133

Published: March 15, 2024

Biofilm-associated infections pose significant challenges in healthcare settings due to their resistance conventional antimicrobial therapies. In the last decade, marine environment has been a precious source of bioactive molecules, including numerous derivatives with antibiofilm activity. this study, we reported synthesis and biological evaluation new series twenty-two thiadiazopyrimidinone obtained by using hybridization approach combining relevant chemical features two important classes compounds: nortopsentin analogues Essramycin derivatives. The synthesized compounds were vitro tested for ability inhibit biofilm formation disrupt mature various bacterial strains. Among compounds, derivative 8j exhibited remarkable dispersal activity against preformed biofilms Gram-positive Gram-negative pathogens, as well towards fungus Candida albicans, showing BIC50 values ranging from 17 40 µg/mL. Furthermore, compound was vivo assayed its toxicity anti-infective effect Galleria mellonella model. results revealed promising combination properties favorable profile treatment severe chronic biofilm-mediated infections.

Language: Английский

Citations

2
Enhancement of Biodegradation and Detoxification of Methylene Blue by Preformed Biofilm of Thermophilic Bacilli on Polypropylene Perforated Balls DOI Creative Commons
Vincenzo Zammuto, Angela Macrì, Eleonora Agostino

et al.

Journal of Marine Science and Engineering, Journal Year: 2024, Volume and Issue: 12(8), P. 1248 - 1248

Published: July 23, 2024

Microbial degradation represents an eco-friendly alternative to traditional physicochemical treatments in removing persistent and toxic environmental pollutants, including synthetic dyes (i.e., methylene blue, MB) employed different industries. The exploitation of thermophilic bacilli, such as those isolated from the shallow hydrothermal vents Eolian Islands (Italy), could provide valuable resources for treatment warm, dye-containing wastewater. In this study, we evaluated ability preformed biofilms on polypropylene perforated balls (BBs) fifteen decolor, degrade, detoxify MB aqueous solutions. Among them, BBs Bacillus licheniformis B3-15 sp. s7s-1 were able decolorize more than 50% saline solution (NaCl 2%), incubated static conditions at 45 °C 48 h. At optimized initial (10 mg L−1 MB, pH 5.2 or 4 s7s-1), two strains enhanced their decolorization potential, reaching 96% 67%, respectively. As indicated by ATR-FTIR spectroscopy, with BB was most efficient degrading Cl–C –NH groups MB. This degraded 40% less undegraded it has no impact bioluminescence Vibrio harveyi, nor growth marine diatom Phaeodactylum tricornutum. Biofilm formed strain be proposed a component bioreactors wastewater concomitantly remediate pollution simultaneously counteract harmful effects aquatic environments.

Language: Английский

Citations

2
Bhargavaea beijingensis a promising tool for bio-cementation, soil improvement, and mercury removal DOI Creative Commons

Megha S. Gadhvi,

Bhumi M. Javia, Suhas Vyas

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Oct. 14, 2024

Microbially Induced Calcite Precipitation (MICP) has emerged as a promising technique for bio-cementation, soil improvement, and heavy metal remediation. This study explores the potential of Bhargavaea beijingensis, urease-producing bacterium, these applications. Six ureolytic bacteria were isolated from calcareous bricks mine screened urease calcite production. B. beijingensis exhibited highest activity precipitation. Urease activity, precipitation, sand solidification, removal efficiency, compressive strength evaluated. It showed significant particularly HgCl

Language: Английский

Citations

2
Ligand-Free Silver Nanoparticles: An Innovative Strategy against Viruses and Bacteria DOI Creative Commons
Maria Vittoria Morone, Annalisa Chianese, Federica Dell’Annunziata

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(4), P. 820 - 820

Published: April 18, 2024

The spread of antibiotic-resistant bacteria and the rise emerging re-emerging viruses in recent years constitute significant public health problems. Therefore, it is necessary to develop new antimicrobial strategies overcome these challenges. Herein, we describe an innovative method synthesize ligand-free silver nanoparticles by Pulsed Laser Ablation Liquid (PLAL-AgNPs). Thus produced, were characterized total X-ray fluorescence, zeta potential analysis, transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA). A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed evaluate nanoparticles’ cytotoxicity. Their evaluated against enveloped herpes simplex virus type 1 (HSV-1) naked poliovirus (PV-1) plaque reduction assays confirmed real-time PCR fluorescence microscopy, showing that interfered with early stage infection. action also examined different bacteria. We observed PLAL-AgNPs exerted a strong effect both methicillin-resistant Staphylococcus aureus (S. MRSA) Escherichia coli (E. coli) producing extended-spectrum β-lactamase (ESBL). In detail, exhibited bacteriostatic S. bactericidal activity E. coli. Finally, proved able inhibit/degrade biofilm

Language: Английский

Citations

1
Production and biological activities of exopolysaccharides synthesized by thermophilic bacilli isolated from hot springs in Türkiye DOI
Mehmet Aytar, Deniz Aktaş Uygun, Gamze Başbülbül

et al.

International Microbiology, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 9, 2024

Language: Английский

Citations

1
EPS T14 from Bacillus licheniformis Prevents Infection of Human Nasal Epithelial Cells by Respiratory Viruses DOI Creative Commons

Gaetana Pezzino,

Alessia Calabrò,

Fabiana Drommi

et al.

ImmunoTargets and Therapy, Journal Year: 2024, Volume and Issue: Volume 13, P. 487 - 499

Published: Sept. 1, 2024

Respiratory viral infections are a leading cause of severe diseases and mortality; therefore, novel treatments effective for their prevention highly requested. Here, we identified broad-spectrum antiviral activity natural exopolysaccharide, EPS T14, purified from marine thermotolerant strain

Language: Английский

Citations

1
Microbial Mat Dominated by Amphora spp. and Their Adaptative Strategies in an Arsenic-Rich Brackish Pond DOI Creative Commons
Eleonora Agostino, Angela Macrì, Vincenzo Zammuto

et al.

Journal of Marine Science and Engineering, Journal Year: 2024, Volume and Issue: 12(11), P. 1966 - 1966

Published: Nov. 1, 2024

Marine diatoms are essential members of both phytoplankton and phytobenthic communities, able to colonize submerged artificial natural surfaces, contributing benthic microbial biomass. Diatoms have developed different adaptative mechanisms cope with various environmental stresses, including high concentrations heavy metals. The aim this study was investigate the arsenic resistance diatoms, isolated from mats collected an arsenic-rich brackish pond (Lake Mergolo della Tonnara, Italy), by evaluating (i) their ability form biofilms in presence arsenite (AsIII) or arsenate (AsV), (ii) variations photosynthetic pigments’ contents (i.e., chlorophyll a c) biofilms. were dominated genus Amphora, isolates affiliated species A. capitellata, coffeaeformis, montana. strains grew better AsV than AsIII, which is generally less toxic. After seven days incubation, each strain exhibited on glass surfaces (25 ppm), montana 27 being most effective (86%) coffeaeformis 26 (74%) AsV. Photosynthetic pigment levels (chlorophyll differed biofilm, poorly reduced AsIII 27, 26, indicating species-specific response stress. Our results indicated that Amphora thriving environment can as As-resistance mechanism, maintain pigments, support functioning ecosystem, favored whereas As producers biofilms, these could be useful develop new strategies remediate pollution.

Language: Английский

Citations

1
Synergistic Antibiofilm Effects of Exopolymers Produced by the Marine, Thermotolerant Bacillus licheniformis B3-15 and Their Potential Medical Applications DOI Creative Commons
Vincenzo Zammuto, Eleonora Agostino, Angela Macrì

et al.

Journal of Marine Science and Engineering, Journal Year: 2023, Volume and Issue: 11(9), P. 1660 - 1660

Published: Aug. 24, 2023

The exopolysaccharide (EPS B3-15) and biosurfactant (BS B3-15), produced by the marine Bacillus licheniformis B3-15, were recently reported to possess different antibiofilm activities, with EPS being more active in preventing adhesion of Pseudomonas aeruginosa Staphylococcus aureus BS destroying their preformed biofilms on surfaces. In this study, synergistic effects two exopolymers bacterial biofilm disruption P. S. evaluated polystyrene, a medical polyvinyl chloride (PVC) device, contact lenses (CLs) order address potential use biomedical applications. To purpose, B3-15 equally combined (1:1 w/w), mixture (BPS was added at concentrations (from 50 300 µg mL−1) times development. Compared each polymer, BPS (300 efficiently reduced polystyrene (65 58%, respectively), PVC devices (62 42%, CLs (39 35%, also combination care solution (88 39%, respectively). Furthermore, able disrupt mature biofilms, acting effectively against (72%) than (6%). low exhibited prevent eradicate biofilms.

Language: Английский

Citations

2
Production and Biological Activities of Exopolysaccharides Synthesized by Thermophilic Bacilli Isolated from Hot Springs in Türkiye DOI
Mehmet Aytar, Deniz Aktaş Uygun, Gamze Başbülbül

et al.

Published: July 29, 2024

Abstract A total of one hundred seven thermophilic bacteria were screened for their ability to produce exopolysaccharides. Nine isolates belonging Geobacillus, Parageobacillus, Aeribacillus and Anoxybacillus genera with highest EPS quantities chosen purified EPSs used biological activity studies. yields selected ranged between 117–419 mg/L. Among the tested EPSs, 61, 106 261 showed antibacterial effect against E. faecalis JH2-2 at a concentration 15 mg/mL. samples had significant antioxidant capacity, especially 134, DPPH radical scavenging 100% 5 mg/mL strongest reducing power. 20, lipid peroxidation inhibition rate 31%. displayed weak alpha amylase when compared standart acarbose. The prebiotic indices 76, 89, 134 found be higher than that inulin, representative carbohydrate all lactic acid in study. All examined inhibited biofilms formed by various depending on test strain. Results indicated remarkable antioxidant, prebiotic, antibiofilm activities. Therefore, characterized this study may have technological applications health food fields.

Language: Английский

Citations

0
Uncovering bacterial-mammalian cell interactions via single-cell tracking DOI Creative Commons
Narendra K. Dewangan,

Sayed Golam Mohiuddin,

Shayne Sensenbach

et al.

BMC Biology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Nov. 11, 2024

The interactions between bacterial pathogens and host cells are characterized by a multitude of complexities, leading to wide range heterogeneous outcomes. Despite extensive research, we still have limited understanding how motility in complex environments impacts their ability tolerate antibiotics adhere mammalian cell surfaces. challenge lies unraveling the complexity these developing quantitative microscopy approaches predict behavior populations.

Language: Английский

Citations

0