Enhancing active ingredient biosynthesis in Chinese herbal medicine: biotechnological strategies and molecular mechanisms DOI Creative Commons
Ziyi Guo, Ning Yang, Delin Xu

et al.

PeerJ, Journal Year: 2025, Volume and Issue: 13, P. e18914 - e18914

Published: Feb. 10, 2025

Background Chinese herbal medicine (CHM) is a fundamental component of traditional medical practice, offering rich source natural remedies with significant therapeutic potential. However, the scarcity active ingredients and complex extraction procedures present substantial challenges to their widespread clinical application. This review aims address this gap by exploring potential modern biotechnological advancements in enhancing biosynthesis these valuable compounds. Methodology The study takes comprehensive approach, delving into chemical composition CHM’s elucidating biosynthetic pathways molecular regulatory mechanisms. Additionally, it surveys recent progress methodologies evaluates engineering strategies aimed at synthetic production. multifaceted analysis forms foundation for examining role biology augmenting ingredient synthesis. Results Our examination provides insights intricate governing formation ingredients, as well networks that underlie processes. Furthermore, highlights techniques, demonstrating ability streamline enhance isolation Engineering approaches production, including metabolic tools, are assessed overcome limitations scale up Conclusions By integrating from biosynthesis, regulation, methodologies, biology, establishes robust theoretical framework production ingredients. proposed practical guidance aim facilitate broader utilization while promoting sustainability accessibility within invaluable medicinal heritage.

Language: Английский

Recent Advancements in the Biomanufacturing of Crocetin and Crocins: Key Enzymes and Metabolic Engineering DOI
Chengpeng Hao, Kai Hu, Jingcong Xie

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 8, 2025

Crocetin and crocins are high-value apocarotenoids recognized for their role as food colorants well numerous industrial therapeutic applications. Biotechnological platforms have the potential to replace traditional plant-based extraction of these compounds with a more sustainable approach. This review first introduced catalytic characteristics key enzymes involved in biosynthetic pathway crocetin crocins, including carotenoid cleavage dioxygenases, aldehyde dehydrogenases, uridine diphosphate glycosyltransferases. Next, we highlighted advanced metabolic engineering strategies aimed at enhancing crocin production, such increasing pool precursors cofactors, protein mining engineering, tuning expression, biosensor, genomic integration, process optimization. Finally, paper proposed tools associated further boosting heterologous production meet commercial-scale demands.

Language: Английский

Citations

0
Advanced Technologies for Large Scale Supply of Marine Drugs DOI Creative Commons

Henar Martínez,

Mercedes Santos,

Lourdes Pedraza

et al.

Marine Drugs, Journal Year: 2025, Volume and Issue: 23(2), P. 69 - 69

Published: Feb. 7, 2025

Marine organisms represent a source of unique chemical entities with valuable biomedical potentialities, broad diversity, and complexity. It is essential to ensure reliable sustainable supply marine natural products (MNPs) for their translation into commercial drugs other products. From structural point view few exceptions, MNPs pharmaceutical importance derive from the so-called secondary metabolism organisms. When production strategies rely on macroorganisms, harvesting or culturing coupled extraction procedures frequently remain only alternative producing these compounds an industrial scale. Their can often be implemented laboratory scale cultures bacterial, fungal, microalgal sources. However, diverse approach, combining traditional methods modern synthetic biology biosynthesis strategies, must considered invertebrate MNPs, as they are usually naturally accumulated in very small quantities. This review offers comprehensive examination various addressing challenges related supply, synthesis, scalability. also underscores recent biotechnological advancements that likely transform current industrial-scale manufacturing pharmaceuticals derived

Language: Английский

Citations

0
Enhancing active ingredient biosynthesis in Chinese herbal medicine: biotechnological strategies and molecular mechanisms DOI Creative Commons
Ziyi Guo, Ning Yang, Delin Xu

et al.

PeerJ, Journal Year: 2025, Volume and Issue: 13, P. e18914 - e18914

Published: Feb. 10, 2025

Background Chinese herbal medicine (CHM) is a fundamental component of traditional medical practice, offering rich source natural remedies with significant therapeutic potential. However, the scarcity active ingredients and complex extraction procedures present substantial challenges to their widespread clinical application. This review aims address this gap by exploring potential modern biotechnological advancements in enhancing biosynthesis these valuable compounds. Methodology The study takes comprehensive approach, delving into chemical composition CHM’s elucidating biosynthetic pathways molecular regulatory mechanisms. Additionally, it surveys recent progress methodologies evaluates engineering strategies aimed at synthetic production. multifaceted analysis forms foundation for examining role biology augmenting ingredient synthesis. Results Our examination provides insights intricate governing formation ingredients, as well networks that underlie processes. Furthermore, highlights techniques, demonstrating ability streamline enhance isolation Engineering approaches production, including metabolic tools, are assessed overcome limitations scale up Conclusions By integrating from biosynthesis, regulation, methodologies, biology, establishes robust theoretical framework production ingredients. proposed practical guidance aim facilitate broader utilization while promoting sustainability accessibility within invaluable medicinal heritage.

Language: Английский

Citations

0