Mimicking the Complexity of Solid Tumors: How Spheroids Could Advance Cancer Preclinical Transformative Approaches

Cancers, Journal Year: 2025, Volume and Issue: 17(7), P. 1161 - 1161

Published: March 30, 2025

Traditional 2D cell culture models present significant limitations in replicating the intricate architecture and microenvironment of vivo solid tumors, which are essential for accurately studying cancer initiation, growth, progression, metastasis. This underscores need development advanced preclinical to accelerate research outcomes. Emerging 3D systems, particularly spheroid models, provide a more realistic representation tumor properties by capturing complex interactions occurring within microenvironment, including extracellular matrix dynamics that influence progression. Among breast remains most frequently diagnosed among women globally leading cause cancer-related mortality. Here we emphasize value cell-derived spheroids revealing differential molecular characteristics understanding during early stages invasion into adjacent tissues. Conclusively, this study urgent adopt platforms, given their contributions pharmaceutical targeting. may well offer transformative approach studies enhance our ability test therapeutic efficiency conditions closely mimic growth progression tumors.

Language: Английский

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Improving the Value Utilization of Tuna Peptide Powder for the Cosmetics Field Through Ozone Oxidation

Marine Drugs, Journal Year: 2025, Volume and Issue: 23(5), P. 191 - 191

Published: April 28, 2025

The existing in vitro and clinical trial evidence supports the health wellness benefits of collagen peptides sourced from various origins. Despite this, research on tuna remains limited. Notably, tuna-derived possess an inherent fishy odor, rendering them unsuitable for direct application humans. This study explores enhancement peptides’ applicability cosmetics through odor mitigation. We developed a dual-phase ozone treatment, employing both dry wet ozone, to deodorize peptide powder, enabling its use cosmetic formulations. deodorized powder can be used cosmetics. optimized nitrification deodorization conditions by adjusting treatment time, concentration, temperature. Sensory evaluation GC-MS analysis confirmed effectiveness removal, offering comprehensive understanding process. findings reveal that ozonation at 50 °C with concentration 99.1 mg/L 40 min significantly reduces powder. n-Hexaldehyde, primary odor-contributing volatile compound, decreased 66.5%, confirming efficacy Moreover, protein activity within remained unaffected, ensuring preservation functional properties. demonstrates oxidation adapting use.

Language: Английский

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Breaking the mold: 3D cell cultures reshaping the future of cancer research

Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12

Published: Nov. 26, 2024

Despite extensive efforts to unravel tumor behavior and develop anticancer therapies, most treatments fail when advanced clinical trials. The main challenge in cancer research has been the absence of predictive models, accurately mimicking tumoral processes response treatments. microenvironment (TME) shows several human-specific physical chemical properties, which cannot be fully recapitulated by conventional 2D cell cultures or

Language: Английский

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Collagen-based materials in male genitourinary diseases and tissue regeneration

Deleted Journal, Journal Year: 2024, Volume and Issue: 6(1)

Published: Dec. 1, 2024

Abstract Male genitourinary dysfunction causes serious physical or mental distress, such as infertility and psychological harm, which leads to impaired quality of life. Current conventional treatments involving drug therapy, surgical repair, tissue grafting have a limited effect on recovering the function fertility organs. To address these limitations, various biomaterials been explored, with collagen-based materials increasingly gaining attention for reconstructing male system due their superior biocompatibility, biodegradability, low antigenicity, biomimetic 3D matrix characteristics, hemostatic efficacy, regeneration capabilities. This review covers recent biomedical applications including treatment erectile dysfunction, premature ejaculation, penile girth enlargement, prostate cancer, Peyronie's disease, chronic kidney etc. Although there are relatively few clinical trials, promising results existing studies animal models reveal bright future in diseases. Graphic

Language: Английский

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Silencing of Epidermal Growth Factor-like Domain 8 Promotes Proliferation and Cancer Aggressiveness in Human Ovarian Cancer Cells by Activating ERK/MAPK Signaling Cascades

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 26(1), P. 274 - 274

Published: Dec. 31, 2024

Ovarian cancer (OC) is the second most common female reproductive and lethal gynecological malignancy worldwide. Most human OCs are characterized by high rates of drug resistance metastasis, leading to poor prognosis. Improving outcomes patients with relapsed treatment-resistant OC remains a challenge. This study aimed investigate role epidermal growth factor-like domain 8 (EGFL8) in examining effects siRNA-mediated EGFL8 knockdown on progression. cells promoted aggressive traits associated progression, including enhanced proliferation, colony formation, migration, invasion, chemoresistance, reduced apoptosis. Additionally, upregulated expression epithelial-mesenchymal transition (EMT) markers (Snail, Twist1, Zeb1, Zeb2, vimentin) stem cell biomarkers (Oct4, Sox2, Nanog, KLF4, ALDH1A1), increased matrix metallopeptidases (MMP-2 MMP-9), genes (MDR1 MRP1), Notch1. Low also correlated prognosis OC. Overall, this provides crucial evidence that inhibits proliferation aggressiveness suppressing ERK/MAPK signaling. Therefore, may serve as valuable prognostic biomarker potential target for developing novel therapies.

Language: Английский

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