Label-Free Extended Gate Field-Effect Transistor for Sensing Microcystin-LR in Freshwater Samples DOI Creative Commons
Sondavid Nandanwar, Songyi Lee, Myeongkee Park

et al.

Sensors, Journal Year: 2025, Volume and Issue: 25(5), P. 1587 - 1587

Published: March 5, 2025

In this study, we developed a label-free biosensor based on aptamer-modified multi-walled carbon nanotube extended gate field-effect transistor (MWCNT-EG-FET) for easy and selective detection of microcystin-LR (MC-LR), prominent cyanotoxin associated with liver damage, bleeding, necrosis. EG-FET had two parts: MOSFET an extended-gate Au/SiO2 electrode, which serves as the sensitive membrane. A custom-designed DNA oligonucleotide (5-NH2-C6-AN6) was used MC-LR-targeting aptamer (MCTA). MWCNTs were functionalized MCTA then stably fixed The MC-LR in freshwater effectively achieved within 5 min by assessing variations electrical resistance that occur due to interactions between MCTA. limit analytical sensitivity found be 0.134 ng/mL 0.024 ng/mL, respectively. membrane could readily discarded if damaged, eliminating need replace main transducer MOSFET. sensor exhibits features such straightforward preparation, swift response, potential miniaturization, ease use, making it attractive candidate future integrated lab-on-chip systems environments.

Language: Английский

Label-Free Extended Gate Field-Effect Transistor for Sensing Microcystin-LR in Freshwater Samples DOI Creative Commons
Sondavid Nandanwar, Songyi Lee, Myeongkee Park

et al.

Sensors, Journal Year: 2025, Volume and Issue: 25(5), P. 1587 - 1587

Published: March 5, 2025

In this study, we developed a label-free biosensor based on aptamer-modified multi-walled carbon nanotube extended gate field-effect transistor (MWCNT-EG-FET) for easy and selective detection of microcystin-LR (MC-LR), prominent cyanotoxin associated with liver damage, bleeding, necrosis. EG-FET had two parts: MOSFET an extended-gate Au/SiO2 electrode, which serves as the sensitive membrane. A custom-designed DNA oligonucleotide (5-NH2-C6-AN6) was used MC-LR-targeting aptamer (MCTA). MWCNTs were functionalized MCTA then stably fixed The MC-LR in freshwater effectively achieved within 5 min by assessing variations electrical resistance that occur due to interactions between MCTA. limit analytical sensitivity found be 0.134 ng/mL 0.024 ng/mL, respectively. membrane could readily discarded if damaged, eliminating need replace main transducer MOSFET. sensor exhibits features such straightforward preparation, swift response, potential miniaturization, ease use, making it attractive candidate future integrated lab-on-chip systems environments.

Language: Английский

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