Meta-analysis of RNA-Seq datasets allows a better understanding of P. tricornutum cellular biology, a requirement to improve the production of Biologics
Scientific Reports,
Journal Year:
2025,
Volume and Issue:
15(1)
Published: Jan. 29, 2025
Abstract
The
marine
diatom
Phaeodactylum
tricornutum
is
currently
used
for
various
industrial
applications,
including
the
pharmaceutical
industry
as
a
cost-effective
cell
biofactory
to
produce
Biologics.
Recent
studies
demonstrated
that
P.
can
functional
monoclonal
antibodies,
such
application
limited
by
production
yield
hinders
industrialization.
Therefore,
it
necessary
understand
and
control
biology
of
improve
Biologics
yield.
Transcriptomic
analyses
have
recently
been
in
mammalian
cells,
especially
titer
productivity.
Hence,
present
work,
we
performed
meta-analysis
seven
publicly
available
RNA-Seq
datasets
from
different
strains
tricornutum,
which
culture
conditions
were
chosen
similar
possible.
We
analyzed
differential
expression
genes
are
involved
biological
processes
well
known
potentially
impact
bioproduction
critical
quality
attributes
N
-glycan
biosynthesis,
protein
export
secretion,
proteasome,
those
encoding
proteases
compared.
results
pave
way
towards
optimizing
highlight
Pt4,
Pt3
Ov
Pt8
seem
be
most
promising
strains.
Language: Английский
Characterization of the VOC Promoter That Is Active Under Low-Salinity Conditions in the Diatom Phaeodactylum tricornutum
Marine Drugs,
Journal Year:
2025,
Volume and Issue:
23(5), P. 185 - 185
Published: April 26, 2025
Microalgae
such
as
Phaeodactylum
tricornutum
are
promising
cell
biofactories
for
the
production
of
high-value
molecules,
including
monoclonal
antibodies
(mAbs).
However,
to
date,
mAbs
in
P.
using
inducible
nitrate
reductase
(NR)
promoter
has
yielded
only
a
limited
amount
mAbs.
Therefore,
identification
robust
that
produces
high
yields
is
crucial
development
cost-effective
expression
system.
To
few
endogenous
promoters
have
been
characterized
tricornutum.
In
this
study,
we
identified
thirty-three
potential
“strong”
based
on
our
previously
published
transcriptomic
data
from
Pt3
strain.
These
putative
sequences
were
cloned
into
an
episomal
vector
and
fused
gene
encoding
enhanced
green
fluorescent
protein
(eGFP).
Their
strength
was
assessed
by
measuring
eGFP
fluorescence,
which
reflects
level
expression.
Of
promoters,
thirteen
able
successfully
drive
Among
them,
best
results
obtained
with
VOC
promoter,
allowed
significant
increase
compared
induced
NR
promoter.
contribute
new
genetic
tools
can
be
used
future
studies
yield
recombinant
proteins
at
industrial
scale.
Language: Английский