Bio-Refinery of Organics into Value-Added Biopolymers: Exploring the Effects of Hydraulic Retention Time and Organic Loading Rate on Biopolymer Harvesting from a Biofilm-Based Process

Toxics, Journal Year: 2025, Volume and Issue: 13(3), P. 183 - 183

Published: Feb. 28, 2025

This study aimed to examine the impacts of hydraulic retention time (HRT) and organic loading rate (OLR) on alginate-like exopolymers’ (ALEs) recovery potential from a biofilm-based process. A lab-scale moving bed biofilm reactor (MBBR) was operated under different HRT (12.0, 6.0, 2.0 h) OLR (1.0, 2.0, 6.0 kg COD/m3/d) conditions. The results demonstrated that reduction in increase had remarkable effects enhancing ALE production improving its properties, which resulted yield increasing 177.8 221.5 mg/g VSS, with protein content rising 399.3 494.3 enhanced alginate purity by 39.8%, corresponding TOC concentration 108.3 157.0 ALE. Meanwhile, illustrate potentials, microbial community compositions MBBR at various operational conditions were also assessed. showed higher relative abundance EPS producers (29.86%) observed an h than 12.0 h, revealing potential. yields crucial terms resource for wastewater reclamation providing effective approach directionally cultivating ALEs.

Language: Английский

Modification of Cotton with Chitosan: Deposition of Copper(II) Sulfate by Complexation Copper Ions

Processes, Journal Year: 2024, Volume and Issue: 12(12), P. 2772 - 2772

Published: Dec. 5, 2024

This study introduces a novel approach for enhancing the functional properties of cotton fibers through complexation copper sulfate, and subsequent combination with chitosan (COT-CuSO4-CTS). Our preliminary investigations focused on development composites as candidate materials coatings antimicrobial properties. The were thoroughly characterized via scanning electron microscopy (SEM) optical microscopy, providing insights into their structural features composition. findings show that modified exhibit potent activity. Specifically, COT-CuSO4 COT-CuSO4-CTS samples demonstrated zones inhibition against both Gram-positive Staphylococcus aureus Gram-negative Escherichia coli, confirming ability to reduce microbial growth significantly. incorporation layer significantly enhanced Ultraviolet Protection Factor (UPF) fabric from 3.37 over 50, indicating exceptional UV shielding capabilities, while copper(II) oxide treatment provided moderate UPF value 14.56. Blood compatibility studies further revealed fabrics influence coagulation parameters, marked prolongation in activated partial thromboplastin time (aPTT) prothrombin (PT) compared untreated cotton. anticoagulant effect is primarily linked presence copper, although addition modulates this response, slightly reducing clotting times alone. Cytotoxicity genotoxicity assessments using Peripheral Mononuclear (PBM) cells indicated was non-toxic non-genotoxic. However, displayed reduction cell viability induced DNA damage, highlighting potential cytotoxic genotoxic effects. Notably, showed lower cytotoxicity than COT-CuSO4-CTS, suggesting reduces overall composite. Furthermore, plasmid relaxation assays interact DNA, exhibiting stronger interaction consistent PBM cells.

Language: Английский