Interdisciplinary cancer research, Journal Year: 2024, Volume and Issue: unknown
Published: Jan. 1, 2024
Language: Английский
E3S Web of Conferences, Journal Year: 2025, Volume and Issue: 602, P. 02003 - 02003
Published: Jan. 1, 2025
The purpose of the research is to compare short peptides from different hydrolysates fish entrails that can inhibit activity angiotensin-converting enzyme (ACE). Fish derived Catfish, Tilapia, and Mackerel were digested by pepsin passed through 3kDa cutoff column. fraction containing shorter than equal catfish hydrolysate has great ability ACE in converting substrate (Furanacroloy-Phe-Gly-Gly, FAPGG) producing FAP GG as products reaction. filtrate had like Captopril, a drug for treating hypertension. entrail was purified using OFFGEL electrophoresis then C18 separated into two fractions these determined inhibitory activity. result showed hydrophilic others hydrophobic possessed against ACE. Those analyzed with LCMS/MS sequencing. results revealed synthesized peptides; ASNLHGV, LFKDLR, PGYALQR, LETAKSR, anti-ACE its substrate.
Language: Английский
Citations
0
E3S Web of Conferences, Journal Year: 2025, Volume and Issue: 602, P. 02005 - 02005
Published: Jan. 1, 2025
In recent decades, Angiotensin-converting enzyme (ACE) inhibitory peptides derived from various proteins have become crucial sources of health-enhancing components for clinical use. Abundant in fish waste entrails can be used to produce ACE peptides. Catfish, Tilapia, and Mackerel were digested by pepsin passed through the 3kDa cutoff column. The protein hydrolysate column C18 analyzed an activities sequenced using LC-MS/MS. Five candidate De novo sequencing was chemically synthesized tested activity. activity result revealed that PGYALQR peptide contains as captopril did. This study aims predict conformation orientation into binding site ACE. Molecular docking analysis AutoDock Vina performed elucidate mechanisms underlying ACE-inhibitory peptide. Computational binds active with −11.2 kcal/mol, forming hydrogen bonds Glu162, Gln281, His353, Ala354, Lys511, His513, Tyr523. comparision, interacted Tyr520, Tyr523 a energy −5.9 kcal/mol. Additionally, interacts Zn (II) ion site, coordinating residues Glu411, His383, His387, which is enhancing its It may contort tetrahedral coordination (II), resulting loss
Language: Английский
Citations
0
Marine Drugs, Journal Year: 2025, Volume and Issue: 23(2), P. 81 - 81
Published: Feb. 13, 2025
Hypertension has been identified as a significant risk factor for cardiovascular disease. Given the prevalence of adverse effects angiotensin-converting enzyme-inhibitory (ACEI) drugs, natural and effective alternatives to these medications need be identified. An investigative study was conducted assess ACEI capacity structural characteristics enzymatic hydrolysates with varying molecular weights derived from squid skin. The amino acid sequences digests were analyzed via Nano LC-MS/MS screened peptides activity using an in silico analysis. Furthermore, docking employed investigate interaction between potential ACE. TPSH-V (MW < 1 kDa) exhibited highest rate ACEI, property attributable its substantial hydrophobic content. Additionally, high temperature pH stability, indicative regular ordering secondary structure. binding modes four novel ACE predicted FHGLPAK, IIAPPERKY, RGLPAYE, VPSDVEF, all which can bind active site hydrogen bonding, VPSDVEF being able coordinate Zn2+. Squid skin constitutes viable resource production peptides.
Language: Английский
Citations
0
Food Research International, Journal Year: 2025, Volume and Issue: 207, P. 116113 - 116113
Published: Feb. 27, 2025
Language: Английский
Citations
0
Interdisciplinary cancer research, Journal Year: 2024, Volume and Issue: unknown
Published: Jan. 1, 2024
Language: Английский
Citations
0