In Vitro Digestion and Gut Microbiota Fermentation of the Anticancer Marine Drug BG136: Stability and Biotransformation Investigation

Marine Drugs, Journal Year: 2025, Volume and Issue: 23(4), P. 156 - 156

Published: April 3, 2025

BG136, a β-1,3/1,6-glucan derived from Durvillaea antarctica, is an injectable anticancer drug and has entered Phase II clinical trials. Rational oral formulation design pivotal focus for our future development research; therefore, elucidating the gastrointestinal fate of BG136 becomes imperative. This study investigated stability biotransformation via in vitro digestion gut microbiota fermentation. The results confirmed BG136’s structural integrity, resistance to degradation highly acid environment by tract enzymes. In contrast, was degraded intestinal bacteria into mid-size fragments along with smaller oligosaccharides. Additionally, process notably elevated total short-chain fatty acids (SCFAs) 38.37 ± 3.29 mM, representing 59.4% increase versus controls (24.08 2.29 mM), propionic exhibiting most substantial increase. Meanwhile, accompanied significant microbial regulation, including beneficial genera (Lactobacillus, Enterococcus) reduction Lachnoclostridium populations. Overall, these findings systematically map bioavailability challenges prebiotic potential highlighting its microbiota-modulating capacity through species-specific ecological providing insights BG136.

Language: Английский

Exploring the In Vivo Fate of β-1, 3/1, 6-Glucan Using Quantitative Tandem Mass Spectrometry Based on a Structure-Specific Fragment

Marine Drugs, Journal Year: 2025, Volume and Issue: 23(4), P. 177 - 177

Published: April 20, 2025

β-glucan, a promising drug candidate for immuno-antitumor therapy, holds tremendous potential clinical applications. However, the absence of highly sensitive quantitative methods polysaccharides, attributed to their complicated chemical structures and susceptibility endogenous interference, has posed significant challenges development. Here, we report reliable analytical strategy quantifying β-1, 3/1, 6-glucan derived from Durvillaea antarctica (BG136) in various biological matrices. This approach integrates targeted depolymerization derivatization, followed by oligosaccharide isomer fingerprinting using ultra-high-performance liquid chromatography-triple quadrupole tandem mass spectrometry (UHPLC-MS/MS). The absolute quantification BG136 relied on abundance structure-specific trisaccharide (Glc-β1, 6-Glc-β1, 3-Glc) generated. methodology not only facilitates prototype-based administration but also exhibits remarkable sensitivity. Following method optimization validation, successfully explored vivo fate across multiple models, including cellular uptake release kinetics, as well preclinical pharmacokinetics. These achievements provide insight into “black box” elimination vivo. work marks first practical application this complex matrices, offering methodological support successful execution Phase I trial.

Language: Английский

Aloe Vera Polysaccharides as Therapeutic Agents: Benefits Versus Side Effects in Biomedical Applications

Polysaccharides, Journal Year: 2025, Volume and Issue: 6(2), P. 36 - 36

Published: May 4, 2025

Aloe Vera (Aloe barbadensis Miller), a historically revered medicinal plant, has garnered great scientific attention due to its polysaccharide-rich bioactive compounds with significant therapeutic potential. This review examines the role of polysaccharides as agents in biomedical applications, highlighting their benefits well risks. Traditionally recognized for anti-inflammatory and antimicrobial effects, which are very important wound healing, relies on polysaccharides, confer immunomodulatory, antioxidant, tissue-regenerative properties. These have shown promise various including skin repair, tissue engineering scaffolds, antiviral therapies, delivery being facilitated via gels, thin films, or oral formulations. explores also mechanisms action applications modern medicine, development topical dietary supplements, innovative systems such films scaffolds. Despite promising benefits, addresses possible side effects too, allergic reactions, gastrointestinal disorders, drug interactions, emphasizing importance understanding these risks safe clinical use. Assessing both advantages challenges polysaccharide medical use, this contributes ongoing dialog regarding integration natural products into practices, ultimately supporting informed decisions application.

Language: Английский