Incidence and Prognostic Significance of Hormonal Receptors and HER2 Status Conversion in Recurrent Breast Cancer: A Retrospective Study in a Single Institute

Medicina, Journal Year: 2025, Volume and Issue: 61(4), P. 563 - 563

Published: March 22, 2025

Background and Objectives: Changes in biomarker status are not rare usually occur an unfavorable direction. Evaluating changes is advantageous for assessing treatment options prognosticating patients. Currently, only a few studies have explored the association between conversion breast cancer relapse. In this study, we sought to determine incidence of receptor conversions patients diagnosed with recurrent comparison their corresponding primary tumors evaluate possible influencing factors. Moreover, aimed assess prognostic significance conversion, if any was detected, Materials Methods: A retrospective cohort study conducted among treated at King Khalid University Hospital, Riyadh, Saudi Arabia. Data were collected from about different parameters hormonal receptors human epidermal growth factor 2 (HER2) tumors. The calculation progression-free survival (PFS)/ relapse-free (RFS) overall (OS) value assessed conversion. Results: Progesterone (PR) had highest (29.9%), followed by HER2 (23%) estrogen (ER) (12.6%). Menopausal concurrent significant factors changes. However, no associations found other clinical factors, including tumor stage histological subtype. analysis revealed statistically differences OS or RFS without Conclusions: Receptor particularly PR HER2, common cancer, emphasizing importance re-biopsy recurrence ensure accurate decisions. While does significantly impact outcomes cohort, further large-scale prospective warranted validate these findings explore implications management.

Language: Английский

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Establishment and Morphological Characterization of Patient-Derived Organoids from Breast Cancer

Biological Procedures Online, Journal Year: 2019, Volume and Issue: 21(1)

Published: June 15, 2019

Patient-derived organoids (PDO) technology represents an emerging tool for the study of tumor biology and drug responsiveness, thus being useful to design personalized medicine approaches. Despite several studies clinical trials are ongoing using PDO from colorectal pancreatic cancer, only few research papers have been published exploiting breast cancer. Here, we developed a new protocol establish surgical biopsy samples. Furthermore, set up also methodologies adopted culture morphological evaluations.Surgical core specimens collected 33 patients with diagnosis cancer processed protocols here described obtaining cancerous healthy mammary tissue (when available) in quick easy way good yields. The more critical aspects influencing yield were characteristic origin (healthy vs tissue) amount material obtained after enzymatic digestion process. Success rate samples was about 20,83%, while this percentage higher (i.e. 87,5%). Also characterization by brightfield transmission electron microscopy has reported.Despite some or specimen is not difficult procedure, establishment stable organoid line able grow replicate, suitable long-term biobank storage, so obvious. A novel, simple procedure obtain proposed achieve high success .

Language: Английский

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Deciphering the role of interferon alpha signaling and microenvironment crosstalk in inflammatory breast cancer

Breast Cancer Research, Journal Year: 2019, Volume and Issue: 21(1)

Published: May 6, 2019

Inflammatory breast cancer (IBC) is the most rare and aggressive subtype of characterized by clusters tumor cells invading lymph vessels, high rates metastasis, resistance to systemic chemotherapy. While significant progress has been made in understanding IBC, survival among IBC patients still only one half that with non-IBC. A major limitation development more specific effective treatments for a lack identifiable molecular alterations are IBC. Emerging evidence suggests nature not but instead driven interplay between autonomous signaling context-dependent cytokine networks from surrounding microenvironment. Recently, type I interferon, specifically interferon alpha signature, identified as pathway upregulated few studies have addressed its role. Activation shown contribute apoptosis cellular senescence also attributed increased migration drug depending on interferon-stimulated genes transcribed. The mechanisms promoting increase expression role plays remain speculative. Current hypotheses suggest immune stromal local microenvironment cascade within cell this activation may further alter This review serves an overview Ideally, future experiments should investigate mechanistic interferons develop efficacious treatment strategies patients.

Language: Английский

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Hyaluronic acid-coated chitosan nanoparticles as targeted-carrier of tamoxifen against MCF7 and TMX-resistant MCF7 cells

Journal of Materials Science Materials in Medicine, Journal Year: 2022, Volume and Issue: 33(2)

Published: Feb. 1, 2022

Tamoxifen (TMX) is used to treat hormone-receptor-positive breast cancers at early stages. This research aimed assess the potential of NPs in targeted delivery TMX against MCF7 and TMX-resistant cancer cell lines. For this purpose, a system including chitosan coated with hyaluronic acid (HA-CS NPs) was created examined vitro. Chitosan were first fabricated loaded using ionic-gelation method prepare drug-delivery system. Then, TMX-loaded CS by crosslinking amino groups carboxylic group acid. The developed then optimized characterized for particle fabrication, drug release, targeting cells. HA-CS size 210 nm its zeta +25 mv. encapsulation efficiency 55%. released from acidic pH (5-6) higher than physiological (7.4). cytotoxic effect on cells significantly free drug. findings confirmed significant suppressive impact compared TMX. Graphical abstract.

Language: Английский

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Circulating Exosomal microRNAs as Predictive Biomarkers of Neoadjuvant Chemotherapy Response in Breast Cancer

Current Oncology, Journal Year: 2022, Volume and Issue: 29(2), P. 613 - 630

Published: Jan. 28, 2022

Neoadjuvant chemotherapy (NACT) is an increasingly used approach for treatment of breast cancer. The pathological complete response (pCR) considered a good predictor disease-specific survival. This study investigated whether circulating exosomal microRNAs could predict pCR in cancer patients treated with NACT.Plasma samples 20 NACT were collected prior to and after the first cycle. RNA sequencing was determine microRNA profiling. Cancer Genome Atlas (TCGA) explore expression patterns survivability candidate miRNAs, their potential targets based on levels copy number variation (CNV) data.Three miRNAs before that (miR-30b, miR-328 miR-423) predicted all analyzed samples. Upregulation miR-127 correlated triple-negative (TNBC). After dose, by exo-miR-141, while miR-34a, exo-miR182, exo-miR-183 non-pCR. A significant correlation between overall survival, subtype, metastasis cancer, suggesting role as predictive biomarkers pCR.If identified this are validated large cohort patients, they might serve non-invasive liquid biopsy monitoring

Language: Английский

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From Chaos to Opportunity: Decoding Cancer Heterogeneity for Enhanced Treatment Strategies

Biology, Journal Year: 2023, Volume and Issue: 12(9), P. 1183 - 1183

Published: Aug. 29, 2023

Cancer manifests as a multifaceted disease, characterized by aberrant cellular proliferation, survival, migration, and invasion. Tumors exhibit variances across diverse dimensions, encompassing genetic, epigenetic, transcriptional realms. This heterogeneity poses significant challenges in prognosis treatment, affording tumors advantages through an increased propensity to accumulate mutations linked immune system evasion drug resistance. In this review, we offer insights into tumor crucial characteristic of cancer, exploring the difficulties associated with measuring quantifying such from clinical biological perspectives. By emphasizing critical nature understanding heterogeneity, work contributes raising awareness about importance developing effective cancer therapies that target distinct elusive trait cancer.

Language: Английский

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Unraveling the Role of Scutellaria baicalensis for the Treatment of Breast Cancer Using Network Pharmacology, Molecular Docking, and Molecular Dynamics Simulation

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(4), P. 3594 - 3594

Published: Feb. 10, 2023

Scutellaria baicalensis is often used to treat breast cancer, but the molecular mechanism behind action unclear. In this study, network pharmacology, docking, and dynamics simulation are combined reveal most active compound in explore interaction between molecule target protein treatment of cancer. total, 25 compounds 91 targets were screened out, mainly enriched lipids atherosclerosis, AGE-RAGE signal pathway diabetes complications, human cytomegalovirus infection, Kaposi-sarcoma-associated herpesvirus IL-17 signaling pathway, small-cell lung measles, proteoglycans immunodeficiency virus 1 hepatitis B. Molecular docking shows that two compounds, i.e., stigmasterol coptisine, could bind well AKT1. According MD simulations, coptisine-AKT1 complex higher conformational stability lower energy than stigmasterol-AKT1 complex. On one hand, our study demonstrates has characteristics multicomponent multitarget synergistic effects other we suggest best effective coptisine targeting AKT1, which can provide a theoretical basis for further drug-like offer mechanisms their roles

Language: Английский

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Cuproptosis-related genes predict prognosis and trastuzumab therapeutic response in HER2-positive breast cancer

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Feb. 5, 2024

Abstract Breast cancer is the most common diagnosed cancer, HER2-positive subtype account for 15% of all breast cancer. HER2-targeted therapy mainstay treatment Cuproptosis a novel form programmed cell death, and caused by mitochondrial lipoylation destabilization iron-sulfur proteins triggered copper, which was considered as key player in various biological processes. However, roles cuproptosis-related genes remain largely unknown. In present study, we constructed prognostic prediction model patients using TCGA database. Dysregulated cells resistant to were analyzed GEO dataset. KEGG pathway, GO enrichment GSEA performed respectively. The immune landscape DLAT CIBERSORT algorithm TIDE algorithm. with high CRGs risk score showed shorter OS. downregulated correlated better survival (HR = 3.30, p 0.022). High expressed associated therapy. Knocking down siRNA increased sensitivity trastuzumab. pathway DEGs indicated that participates pathways organelle fission, chromosome segregation, nuclear division, hormone-mediated signaling regulation intracellular estrogen receptor condensed PPAR pathway. There negative correlation between expression (r − 0.292, < 0.001), means an indicator immunotherapy. conclusion, our study four signature identified independent factor patients.

Language: Английский

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Targeting notch-related lncRNAs in cancer: Insights into molecular regulation and therapeutic potential

Pathology - Research and Practice, Journal Year: 2024, Volume and Issue: 257, P. 155282 - 155282

Published: April 1, 2024

Language: Английский

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Clinicopathological and epidemiological significance of breast cancer subtype reclassification based on p53 immunohistochemical expression

npj Breast Cancer, Journal Year: 2019, Volume and Issue: 5(1)

Published: July 25, 2019

Abstract TP53 mutations are common in breast cancer and typically associated with more aggressive tumor characteristics, but little is known about the clinicopathological epidemiological relevance of p53 protein expression, a mutation surrogate, subtypes. In this study 7226 Chinese women invasive cancer, we defined subtypes using immunohistochemical (IHC) measures hormone receptors HER2 conjunction histologic grade. expression status was then used to further stratify into p53-positive p53-negative. Odds ratios (ORs) 95% confidence intervals (CIs) case-only logistic regression analyses were examine heterogeneity across different The frequency varied by subtype, being lowest luminal A-like highest triple-negative HER2-enriched ( P -value < 0.01). B-like/HER2-negative subtypes, positivity early-onset tumors, high grade, proliferative index, basal marker (CK5/6 EGFR) expression. Further, compared A-like/p53-negative patients, A-like/p53-positive patients likely be parous [adjusted OR vs. nulliparous = 2.67 (1.60, 4.51); 0.01] have breastfed ever never 1.38 (1.03, 1.85); 0.03]. not examined clinical risk factors other Overall, these findings suggest that which readily available many settings, can identify phenotypes distinct implications.

Language: Английский

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