Non-Targeted Metabolomic Analysis of Arabidopsis thaliana (L.) Heynh: Metabolic Adaptive Responses to Stress Caused by N Starvation DOI Creative Commons
Jorge David Cadena-Zamudio, Juan L. Monribot–Villanueva, Claudia-Anahí Pérez-Torres

et al.

Metabolites, Journal Year: 2023, Volume and Issue: 13(9), P. 1021 - 1021

Published: Sept. 18, 2023

As sessile organisms, plants develop the ability to respond and survive in changing environments. Such adaptive responses maximize phenotypic metabolic fitness, allowing adjust their growth development. In this study, we analyzed plasticity of Arabidopsis thaliana response nitrate deprivation by untargeted metabolomic analysis using wild-type (WT) genotypes loss-of-function nia1/nia2 double mutant. Secondary metabolites were identified seedlings grown on a hydroponic system supplemented with optimal or limiting concentrations N (4 0.2 mM, respectively) harvested at 15 30 days age. Then, spectral libraries generated from shoots roots both ionization modes (ESI +/-) compared. Totals 3407 4521 signals (m/z_rt) obtained ESI+ ESI- modes, respectively. Of these, approximately 50 65% as differentially synthetized/accumulated. This led presumptive identification 735 KEGG codes (metabolites) belonging 79 pathways. The WT 4 mM favor synthesis/accumulation strongly related growth. contrast, for mutant (similar genotype N), synthetized/accumulated help cope stress, regulating oxidative stress preventing programmed cell death, meaning that under starvation compromise prioritize defensive response.

Language: Английский

Metabolomics-centered mining of plant metabolic diversity and function: Past decade and future perspectives DOI Creative Commons
Shuangqian Shen, Chuansong Zhan, Chenkun Yang

et al.

Molecular Plant, Journal Year: 2022, Volume and Issue: 16(1), P. 43 - 63

Published: Sept. 16, 2022

Language: Английский

Citations

151
Applications of Metabolomics for the Elucidation of Abiotic Stress Tolerance in Plants: A Special Focus on Osmotic Stress and Heavy Metal Toxicity DOI Creative Commons
Manamele D. Mashabela,

Priscilla Masamba,

Abidemi Paul Kappo

et al.

Plants, Journal Year: 2023, Volume and Issue: 12(2), P. 269 - 269

Published: Jan. 6, 2023

Plants undergo metabolic perturbations under various abiotic stress conditions; due to their sessile nature, the network of plants requires continuous reconfigurations in response environmental stimuli maintain homeostasis and combat stress. The comprehensive analysis these features will thus give an overview plant responses strategies applied mitigate deleterious effects conditions at a biochemical level. In recent years, adoption metabolomics studies has gained significant attention growing technological advances analytical biochemistry (plant metabolomics). complexity landscape sophisticated, advanced methods. As such, advancements field have been realized, aided much by development refinement separatory techniques, including liquid gas chromatography (LC GC), often hyphenated state-of-the-art detection instruments such as mass spectrometry (MS) or nuclear resonance magnetic (NMR) spectroscopy. Significant developments techniques are briefly highlighted this review. enormous progress made far also comes with dawn Internet Things (IoT) technology housed machine learning (ML)-based computational tools for data acquisition, mining, 4IR era allowing broader coverage biological interpretation cellular status varying conditions. Thus, scientists can paint holistic roadmap predictive models metabolite-guided crop improvement. current review outlines application related elucidating stress, mainly focusing on heavy metal toxicity subsequent osmotic tolerance.

Language: Английский

Citations

32
Ethiopian coffee: Production systems, geographical origin traceability, and European Union Deforestation Regulation directive compliance DOI Creative Commons
Markos Makiso Urugo,

Mohammed Worku,

Yetenayet B. Tola

et al.

Journal of Agriculture and Food Research, Journal Year: 2025, Volume and Issue: unknown, P. 101695 - 101695

Published: Jan. 1, 2025

Language: Английский

Citations

1
Exploring the intricacies of plant growth promoting rhizobacteria interactions: an omics review DOI Creative Commons

Kamogelo Mmotla,

Nompumelelo R. Sibanyoni,

Farhahna Allie

et al.

Annals of Microbiology, Journal Year: 2025, Volume and Issue: 75(1)

Published: Feb. 4, 2025

Language: Английский

Citations

1
Multi-Omics Approaches Against Abiotic and Biotic Stress—A Review DOI Creative Commons

Venkatramanan Varadharajan,

R. Radhika,

Pandiyan Muthuramalingam

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(6), P. 865 - 865

Published: March 10, 2025

Plants face an array of environmental stresses, including both abiotic and biotic stresses. These stresses significantly impact plant lifespan reduce agricultural crop productivity. Abiotic such as ultraviolet (UV) radiation, high low temperatures, salinity, drought, floods, heavy metal toxicity, etc., contribute to widespread losses globally. On the other hand, those caused by insects, fungi, weeds, further exacerbate these challenges. stressors can hinder systems at various levels, molecular, cellular, development processes. To overcome challenges, multi-omics computational approaches offer a significant tool for characterizing plant’s biomolecular pool, which is crucial maintaining homeostasis signaling response changes. Integrating multiple layers omics data, proteomics, metabolomics, ionomics, interactomics, phenomics, simplifies study resistance mechanisms. This comprehensive approach enables regulatory networks pathway maps, identifying potential targets improving through genetic engineering or breeding strategies. review highlights valuable insights from integrating unravel stress responses factors. By decoding gene regulation transcriptional networks, techniques reveal critical mechanisms underlying tolerance. Furthermore, role secondary metabolites in bio-based products enhancing mitigation discussed. Genome editing tools promising strategies resilience, evidenced successful case studies combating stressors. whole, this extensively discusses advanced that aids understanding molecular basis developing novel improve crops’ organisms’ resilience

Language: Английский

Citations

1
Metabolic and Physiological Changes in the Roots of Two Oat Cultivars in Response to Complex Saline-Alkali Stress DOI Creative Commons
Yugang Gao,

Yongling Jin,

Wei Dong Guo

et al.

Frontiers in Plant Science, Journal Year: 2022, Volume and Issue: 13

Published: March 29, 2022

Saline-alkali stress is a major abiotic factor in agricultural productivity. Oat (Avena sativa L.) saline-alkali tolerant crop species. However, molecular mechanisms of tolerance oats remain unclear. To understand the physiological and underlying seedling oats, phenotypic metabolic responses two oat cultivars, Baiyan7 (BY, cultivar) Yizhangyan4 (YZY, sensitive cultivar), were characterized under conditions. Compared with YZY, BY showed better adaptability to stress. A total 151 96 differential metabolites induced by identified roots respectively. More detailed analyses indicated that enhancements energy metabolism accumulations organic acids active strategies roots, response complex The utilized sugars via sugar consumption more effectively, while amino strengthened upregulated lignin might be positive stress, which led higher osmotic adjustment solute concentrations cell growth. YZY mainly used soluble flavonoids combined form glycosides, as regulatory substances or antioxidant substances, cope different cultivars provided an important theoretical basis for understanding increased our knowledge plant regulation Meanwhile, some related metabolites, such proline, betaine, p-coumaryl alcohol, can also candidates screening cultivars.

Language: Английский

Citations

30
Computational Metabolomics Tools Reveal Metabolic Reconfigurations Underlying the Effects of Biostimulant Seaweed Extracts on Maize Plants under Drought Stress Conditions DOI Creative Commons
Morena M. Tinte, Keabetswe Masike, Paul A. Steenkamp

et al.

Metabolites, Journal Year: 2022, Volume and Issue: 12(6), P. 487 - 487

Published: May 27, 2022

Drought is one of the major abiotic stresses causing severe damage and losses in economically important crops worldwide. decreases plant water status, leading to a disruptive metabolic reprogramming that negatively affects growth yield. Seaweed extract-based biostimulants show potential as sustainable strategy for improved crop health stress resilience. However, cellular, biochemical, molecular mechanisms governing agronomically observed benefits seaweed extracts on plants are still poorly understood. In this study, liquid chromatography-mass spectrometry-based untargeted metabolomics approach combined with computational strategies was applied unravel 'stamps' define effects greenhouse-grown maize (Zea mays) under drought conditions. We mass spectral networking, substructure discovery, chemometrics, pathway analyses mine interpret generated data. The results showed application induced alterations different pathways primary secondary metabolism, such phenylpropanoid, flavonoid biosynthesis, fatty acid amino acids pathways. These changes involved increasing levels phenylalanine, tryptophan, coumaroylquinic acid, linolenic metabolites. known some various biochemical physiological events lead enhanced resistance traits. latter include root growth, alleviation oxidative stress, water, nutrient uptake. Moreover, study demonstrates use networking annotating metabolome. Furthermore, reveal remodeling subsequently readjusting towards alleviation, example, by height diameter through foliar application. Such insights add ongoing efforts elucidating modes action biostimulants, extracts. Altogether, our contributes fundamental scientific knowledge necessary development industry aiming food security.

Language: Английский

Citations

29
Phytoremediation Strategies for Mitigating Environmental Toxicants DOI Creative Commons
Mahendra Aryal

Heliyon, Journal Year: 2024, Volume and Issue: 10(19), P. e38683 - e38683

Published: Oct. 1, 2024

Language: Английский

Citations

6
Tools and Techniques Used at Global Scale Through Genomics, Transcriptomics, Proteomics, and Metabolomics to Investigate Plant Stress Responses at the Molecular Level DOI
Rizky Dwi Satrio, Miftahul Huda Fendiyanto,

Miftahudin Miftahudin

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

6
Deciphering nutrient stress in plants: integrative insight from metabolomics and proteomics DOI
Abiodun Yusuff Moshood, Mukhtar Iderawumi Abdulraheem, Linze Li

et al.

Functional & Integrative Genomics, Journal Year: 2025, Volume and Issue: 25(1)

Published: Feb. 15, 2025

Language: Английский

Citations

0