The Influence of Lactobacillus spp. Secondary Metabolites Isolated from Immature Egyptian Honey on Human Pathogens, Transcription of Virulence Genes and Lung Cancer

Indian Journal of Microbiology, Journal Year: 2024, Volume and Issue: 64(2), P. 671 - 682

Published: March 4, 2024

Abstract This work aimed to isolate, and identify Lactic Acid Bacteria LAB from Egyptian immature citrus honey, characterize their secondary metabolites, as well determine the antibacterial activities transcription of virulence genes (stx1, stx2, eae) influenced by these bacterial metabolites. From twenty hives, bee honey samples were taken. Traditional cultural biochemical testing used, followed molecular confirmation. Further, isolates' cytotoxic properties investigated. 16S rRNA gene sequencing assessed and, two lactic acid isolates identified Lactobacillus acidophilus Ch 2 Levilactobacillus brevis 1 . Both have good antagonistic action against clinical pathogens, with exhibiting best activity all indicator pathogens examined. When compared untreated cancer cells, demonstrated significant activity. cell viability percentages 39.5% 18.76%, respectively. Furthermore, when exposed Shiga-producing Escherichia coli (STEC) expression was suppressed. To a high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF) approach developed. Twenty-seven metabolites diverse chemical classes discovered in crude extracts anticancer characteristics. is first thorough investigation on metabolic profile isolated findings suggested that or could be used food sector medicinal alternatives biocontrol agent.

Language: Английский

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Evaluation of Antimicrobial Activity by Marine Nocardiopsis dassonvillei against Foodborne Listeria monocytogenes and Shiga Toxin-Producing Escherichia coli

Microorganisms, Journal Year: 2023, Volume and Issue: 11(10), P. 2539 - 2539

Published: Oct. 12, 2023

The emergence of multidrug-resistant pathogens creates public health challenges, prompting a continuous search for effective novel antimicrobials. This study aimed to isolate marine actinomycetes from South Africa, evaluate their in vitro antimicrobial activity against Listeria monocytogenes and Shiga toxin-producing Escherichia coli, characterize mechanisms action. Marine were isolated identified by 16S rRNA sequencing. Gas chromatography-mass spectrometry (GC-MS) was used identify the chemical constituents bioactive actinomycetes' secondary metabolites. Antibacterial metabolites assessed broth microdilution method, mode actions predicted using computational docking. While five strains showed antibacterial during primary screening, only Nocardiopsis dassonvillei strain SOD(B)ST2SA2 exhibited screening activity. GC-MS major compounds: 1-octadecene, diethyl phthalate, pentadecanoic acid, 6-octadecenoic trifluoroacetoxy hexadecane. SOD(B)ST2SA2's extract demonstrated minimum inhibitory concentration bactericidal concentration, ranging 0.78-25 mg/mL 3.13 > 25 mg/mL, respectively. Diethyl phthalate displayed lowest bacterial protein-binding energies (kcal/mol): -7.2, dihydrofolate reductase; -6.0, DNA gyrase B; -5.8, D-alanine:D-alanine ligase. Thus, N. is potentially good source compounds that can be control STEC monocytogenes.

Language: Английский

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Phylogenetic Analysis and Bioactivity of Soil-Derived Nocardiopsis Species: Antibacterial and Anticancer Potentials against MCF7 and HCT16 Cell

Journal of Bioscience and Applied Research/Journal of Bioscience and Applied Research , Journal Year: 2023, Volume and Issue: 0(0), P. 102 - 114

Published: Aug. 27, 2023

Soil microorganisms, especially actinomycetes from the Actinobacteria species, are a valuable source of therapeutic compounds.They known for producing abundant bioactive metabolites.Given rise drugresistant pathogens, exploring new sources novel antibacterial agents is crucial.In this study, bacterial strain, Nocardiopsis dassonvillei DSM 43111, was isolated sandy soil in Giza, Egypt.This study represents first investigation into biological effects which tested against five species to assess its bactericidal properties.The explored cytotoxic on normal BHK cell line and anticancer activity MCF7 HCT16 lines.Nocardiopsis 43111 demonstrated noteworthy lines, suggesting promise cancer therapy.However, slight cytotoxicity observed cells, indicating need further compounds actinomycete metabolites.Additionally, disc diffusion tests confirmed strain's strong potential toward studied infectious pathogens.In light these results, microbes have tremendous as medicinal molecules.Moreover, current introduces promising candidate, warranting exploration drug discovery endeavors.

Language: Английский

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