Ecotoxicology and Environmental Safety,
Journal Year:
2024,
Volume and Issue:
271, P. 115940 - 115940
Published: Jan. 12, 2024
Coptis
chinensis
Franch
is
a
perennial
herb
from
the
Ranunculaceae
family
with
long
history
of
medicinal
use.
As
part,
rhizome
coptis
often
accumulates
excessive
cadmium
(Cd)
even
at
low
concentrations
in
soil,
which
not
only
compromises
its
safety
but
also
raises
concerns
about
adverse
effects
on
human
health.
Therefore,
effective
strategies
are
needed
to
mitigate
this
accumulation
and
ensure
safe
use
traditional
medicine.
This
study
utilized
transcriptome
profiling
physiological
analysis
explore
molecular
mechanisms
associated
ecological
significance
active
Cd
C.
chinensis.
The
response
was
assessed
through
RNA
sequencing,
determination
isoquinoline
alkaloid
measurement
using
roots,
stems,
leaves.
revealed,
total
2667,
2998,
or
2815
up-regulated
deferentially
expressed
genes
stems
leaves
exposure.
Furthermore,
we
identified
phenylpropanoid
biosynthesis
as
key
pathways
exposure,
suggests
that
may
improve
tolerance
regulating
pathway.
Under
plant-pathogen
interaction
pathway,
indicates
upregulation
involved
could
enhance
disease
resistance
WGCNA
WRKY8
(Cluster-55763.31419)
WRKY47
(Cluster-55763.221590)
potential
regulators
secondary
metabolic
synthesis
pathway
triggered
by
Cd.
berberine,
coptisine,
palmatine,
epiberberine
demonstrated
simulated
four
alkaloids
roots.
our
presented
expression
profiles
revealed
significant
metal
transport
detoxification
suggested
possible
mechanism
cope
accumulation.
knowledge
provides
new
insight
into
gene
manipulation
for
controlling
accumulation,
enhancing
promoting
metabolites
properties
other
plant
species.
The
emergence
of
MDR
in
plant
pathogens
is
a
threat
to
disease
management
and
leads
the
use
excessive
fungicides.
Botrytis
cinerea
particular
concern
because
its
has
widely
emerged
field.
Understanding
genesis
first
step
for
controlling
MDR.
In
this
study,
contribution
PSMs
been
examined.
Effective
pathogen
agroecosystems
relies
on
better
understanding
how
it
copes
with
phytochemicals
or
Chemical and Biological Technologies in Agriculture,
Journal Year:
2024,
Volume and Issue:
11(1)
Published: April 2, 2024
Abstract
Background
The
exploration
of
EOs
and
their
impact
on
primary
metabolites
in
agricultural
products,
such
as
grapes,
holds
significant
importance
the
post-harvest
preservation
enhancement
berry
quality.
Therefore,
our
research
aimed
to
dissect
effects
various
EO
treatments
biochemical
composition
specifically
examining
alterations
sugar,
organic
acid,
amino
acid
levels.
Results
Our
findings
highlighted
that
OF
treatment,
a
combination
Eugenol
Botrytis
cinerea
,
significantly
escalated
sugar
content,
with
remarkable
increases
sucrose,
glucose,
fructose
compared
control
group.
SF
treatment
led
highest
levels
certain
acids,
including
tartaric,
butyric,
malonic
whereas
TO
prominently
augmented
acids
tyrosine
phenylalanine.
Utilizing
PCA,
study
effectively
captured
variability
within
dataset,
revealing
distinct
clusters
correlations
specific
metabolites.
PCA
biplot
underscored
influence
metabolic
profile
grape
berries,
like
OSF,
TO,
TOSF
demonstrating
close
associations
suggesting
positive
correlations.
Conclusion
current
results
indicate
substantial
resulting
enhanced
fruit
quality
extended
shelf
life.
Variations
among
different
highlight
potential
for
improving
characteristics.
To
sum
up,
these
are
valuable
food
industries,
showcasing
utility
natural
preservatives
enhancers
cultivation
storage.
Graphical
Journal of Fungi,
Journal Year:
2024,
Volume and Issue:
10(4), P. 261 - 261
Published: March 29, 2024
The
necrotrophic
pathogen
Botrytis
cinerea
infects
a
broad
range
of
plant
hosts
and
causes
substantial
economic
losses
to
many
crops.
Although
resistance
procymidone
has
been
observed
in
the
field,
it
remains
uncertain
why
is
usually
involved
multidrug
(MDR)
together
with
other
fungicides.
Nine
mutants
derived
from
B.
strain
B05.10
through
domestication
exhibited
high
factors
(RFs)
against
both
fludioxonil.
However,
fitness
was
reduced
compared
their
parental
strain,
showing
non-sporulation
moderate
virulence.
Furthermore,
RFs
these
fungicides,
such
as
azoxystrobin,
fluazinam,
difenoconazole,
pyrimethanil,
ranged
10
151,
indicating
occurrence
MDR.
Transcriptive
expression
analysis
using
quantitative
polymerase
chain
reaction
(qPCR)
revealed
that
overexpressed
ABC
transporter
genes,
ranging
2
93.7-fold.
These
carried
single-point
mutations
W647X,
R96X,
Q751X
within
BcBos1
by
DNA
sequencing.
alterations
conferred
fungicides
mutants.
Molecular
docking
suggested
distinct
interactions
between
Bos1
standard
or
resistant
mutants,
suggesting
higher
affinity
former
towards
binding
fungicide.
This
study
provides
comprehensive
understanding
biological
characteristics
conducts
an
initial
investigation
into
its
fungicide
traits,
providing
reference
for
field.
Plants,
Journal Year:
2023,
Volume and Issue:
12(24), P. 4087 - 4087
Published: Dec. 6, 2023
Botrytis
cinerea
(B.
cinerea)
causes
gray
mold
disease
(GMD),
which
results
in
physiological
disorders
plants
that
decrease
the
longevity
and
economic
value
of
horticultural
crops.
To
prevent
spread
GMD
during
distribution,
a
rapid,
early
detection
technique
is
necessary.
Thermal
imaging
has
been
used
for
various
plants,
including
potted
roses;
however,
its
application
to
cut
roses,
have
high
global
demand,
not
established.
In
this
study,
we
investigated
utility
thermal
B.
infection
roses
by
monitoring
changes
petal
temperature
after
fungal
inoculation.
We
examined
effects
on
postharvest
quality
treated
with
different
concentrations
conidial
suspensions
chemicals.
decreased
flower
opening,
disrupted
water
balance,
vase
life
roses.
Additionally,
average
rose
petals
was
higher
infected
flowers
than
non-inoculated
flowers.
One
day
before
appearance
necrotic
symptoms
(day
1
period),
significantly
higher,
1.1
°C,
The
GMD-induced
increase
associated
mRNA
levels
genes
related
ethylene,
reactive
oxygen
species,
transport.
Furthermore,
caused
strongly
correlated
symptom
severity
biomass.
A
multiple
regression
analysis
revealed
incidence
positively
one
symptoms.
These
show
thermography
an
effective
evaluating
possible
method
industry.
Applied Sciences,
Journal Year:
2024,
Volume and Issue:
14(21), P. 10061 - 10061
Published: Nov. 4, 2024
Podosphaera
xanthii
is
the
main
causal
agent
of
powdery
mildew
(PM)
disease
for
Cucurbita
pepo.
Disease
control
attained
principally
by
applications
chemical
fungicides,
along
with
parallel
use
tolerant
crop
varieties
and
alternate
application
elicitors
to
development
resistance.
To
get
insight
into
C.
pepo
molecular
responses
P.
infection
elicitor
treatment
we
studied
proteomic
profile
differences
at
phyllosphere
a
zucchini
cultivar
susceptible
PM,
onset
(PX)
after
Reynoutria
sachalinensis
(RS)
plant
extract,
respectively,
using
nano-LC-HRMS/MS,
Q-Exactive-Orbitrap
approach.
Analysis
peptide
sequences
regarding
four
groups
(Control;
PX;
RS;
RSPX
(PX-infected
priorly
treated
RS))
resulted
in
2070
CuGenDB
annotations.
Three
comparisons
(treatments
vs.
Control)
encompassed
most
Differentially
Expressed
Proteins
(DEPs).
In
these
three
comparisons,
KEGG
Gene
Ontology
functional
analyses
highlighted
unique
differentially
enriched
pathways—some
which
included
highly
expressed
proteins—in
PX-related
(proteasome,
pentose
phosphate
pathway,
carbon
fixation),
RS-related
(biosynthesis
secondary
metabolites,
flavonoids,
starch
sucrose
metabolism),
RSPX-related
(pyruvate
metabolism
polycomb
repressive
complex)
suggesting
distinct
mechanisms
early
modulated
PX
RS.
Furthermore,
out
six
thiamine
pathway
was
found
be
enriched,
pivotal
role
PX-induced
responses.
