Mechanism Studies on Α-Glucosidase Inhibition and Glycemic Regulation in Insulin Resistance Hepg2 Cells of Inotodiol from Mushroom Inonotus Obliquus

Published: Jan. 1, 2025

Inonotus obliquus is a medicinal fungus that has been favored for its anti-diabetic benefits in China thousands of years. Inotodiol characteristic triterpenoid isolated from obliquus. The inactivation effect and mechanism α-glucosidase induced by inotodiol were investigated using fluorescence, FTIR, CD spectroscopy, molecular dynamics simulation. could effectively inhibit activity (IC50 = 1.09 ± 0.08 mmol/L) reversible noncompetitive manner. interacted with via static quenching process binding constant 1.88 × 103 mol/L at 298 K. Molecular simulation revealed mainly hydrogen bonding the complex system was stable. also increase glucose consumption glycogen synthesis insulin-resistant HepG2 cells through IRS-1/PI3K/AKT-mediated GSK-3β signaling pathway. Overall, this study suggested potential to be an agent or functional food diabetics.

Language: Английский

Integrated network pharmacology and metabolomics analysis to reveal the potential mechanism of Ershen Wan in ameliorating ulcerative colitis

Journal of Ethnopharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 119690 - 119690

Published: March 1, 2025

Language: Английский

Molecular Mechanism of Yangshen Maidong Decoction in the Treatment of Chronic Heart Failure based on Network Pharmacology, Molecular Docking, and Molecular Dynamics Simulations

Cell Biochemistry and Biophysics, Journal Year: 2024, Volume and Issue: 82(2), P. 1433 - 1451

Published: May 16, 2024

Language: Английский