Sustainability, Journal Year: 2024, Volume and Issue: 16(23), P. 10650 - 10650

Published: Dec. 5, 2024

Due to environmental pollution and the depletion of fossil fuels, there is growing interest in development use biofuels as environmentally friendly alternatives. One most promising biohydrogen, hydrogen produced through sustainable processes using microorganisms such bacteria algae. interesting for production Ralstonia eutropha H16, known its ability produce oxygen-tolerant hydrogenases. These enzymes play a crucial role biohydrogen metabolism production. The aim this work was determine optimal conditions (reactor type synthetic medium composition) cultivation R. H16. culture media contained different concentrations fructose glycerol (mono- or double-substrate cultivation) experiments were carried out batch reactor. initial with 4 g/L at pH 7, T = 30 °C, 120 rpm. mathematical model, consisting growth kinetics (described by Monod’s model) corresponding mass balances, proposed. developed model validated two independent substrate concentrations: 2 one 1 second. In order propose procedure future research, simulations performed reactor types (batch, fed-batch, continuous stirred tank reactors) concentrations. successful experiment glycerol, where γX 0.485 ± 0.001 biomass achieved. Further calculations showed that would be higher (at γG 6.358 g/L, 1.311 should achieved after 200 h when fed-batch (γX 0.944 cultivation).

Language: Английский