Review on new approach methods to gain insight into the feto-maternal interface physiology DOI Creative Commons
Ramkumar Menon, Louis J. Muglia,

Lisa Hara Levin

et al.

Frontiers in Medicine, Journal Year: 2023, Volume and Issue: 10

Published: Nov. 30, 2023

Non-human animals represent a large and important feature in the history of biomedical research. The validity their use, terms reproducible outcomes translational confidence to human situation, as well ethical concerns surrounding that have been remain controversial topics. Over last 10 years, communities developing microphysiological systems (MPS) produced new approach method (NAMs) such organoids organs-on-a-chip. These alternative methodologies shown indications greater reliability translatability than animal use some areas, more humane substitutions for these settings, - with continued scientific effort may change conduct basic research, clinical studies, safety testing, drug development. Here, we present an introduction human-relevant suggest how suite pregnancy associated feto-maternal interface system-oriented NAMs be integrated reliable partial-/full replacements investigators, significantly aid animal-/environmental welfare, improve healthcare outcomes.

Language: Английский

3D cancer models: One step closer to in vitro human studies DOI Creative Commons

Nicoletta Manduca,

Ester Maccafeo,

Ruggero De Maria

et al.

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: April 11, 2023

Cancer immunotherapy is the great breakthrough in cancer treatment as it displayed prolonged progression-free survival over conventional therapies, yet, to date, only a minority of patients. In order broad clinical applicability some roadblocks need be overcome, first among all lack preclinical models that faithfully depict local tumor microenvironment (TME), which known dramatically affect disease onset, progression and response therapy. this review, we provide reader with detailed overview current 3D developed mimick complexity dynamics TME, focus on understanding why TME major target anticancer We highlight advantages translational potentials spheroids, organoids immune Tumor-on-a-Chip modeling therapeutic response, while outlining pending challenges limitations. Thinking forward, possibility integrate know-hows micro-engineers, immunologists, pharmaceutical researchers bioinformaticians meet needs clinicians interested using these platforms high fidelity for patient-tailored drug discovery.

Language: Английский

Citations

43
Modeling the neuroimmune system in Alzheimer’s and Parkinson’s diseases DOI Creative Commons
Wendy Balestri, Ruchi Sharma, Victor Allisson da Silva

et al.

Journal of Neuroinflammation, Journal Year: 2024, Volume and Issue: 21(1)

Published: Jan. 23, 2024

Abstract Parkinson’s disease (PD) and Alzheimer’s (AD) are neurodegenerative disorders caused by the interaction of genetic, environmental, familial factors. These diseases have distinct pathologies symptoms that linked to specific cell populations in brain. Notably, immune system has been implicated both diseases, with a particular focus on dysfunction microglia, brain’s resident cells, contributing neuronal loss exacerbating symptoms. Researchers use models neuroimmune gain deeper understanding physiological biological aspects these how they progress. Several vitro vivo models, including 2D cultures animal utilized. Recently, advancements made optimizing existing developing 3D organ-on-a-chip systems, holding tremendous promise accurately mimicking intricate intracellular environment. As result, represent crucial breakthrough transformation current treatments for PD AD offering potential conducting long-term disease-based modeling therapeutic testing, reducing reliance significantly improving viability compared conventional models. The application research marks prosperous step forward, providing more realistic representation complex interactions within system. Ultimately, refined aim aid quest combat mitigate impact debilitating patients their families.

Language: Английский

Citations

17
Actuation for flexible and stretchable microdevices DOI Open Access

Uditha Roshan,

Amith Mudugamuwa, Haotian Cha

et al.

Lab on a Chip, Journal Year: 2024, Volume and Issue: 24(8), P. 2146 - 2175

Published: Jan. 1, 2024

This review paper provides a comprehensive overview of the state-of-the-art actuation mechanisms for flexible and stretchable microdevices.

Language: Английский

Citations

13
Emerging microfluidics for the modeling and treatment of arthritis DOI Creative Commons

Nengjie Yang,

Chi‐Kuang Sun, Chen Dong

et al.

Engineered Regeneration, Journal Year: 2024, Volume and Issue: 5(2), P. 153 - 169

Published: March 7, 2024

Microfluidic is a technology that allows the precise control of fluid in micro-channel. With its advantages high throughput and low cost, microfluidic has achieved good performance various fields recent years. Arthritis general term for variety joint diseases, which can be clinically manifested as pain swelling, seriously affecting people's physical mental health. At present, causes arthritis disease are still unknown, existing models treatment methods limited, so more treatments need to developed. organ chip cutting-edge build bionic human model, reflect structure function characteristics organs by simulating physiological environment tissues cells vitro. This paper reviews application modeling arthritis, hoping open up new vision study arthritis.

Language: Английский

Citations

9
Cancer-on-chip: a 3D model for the study of the tumor microenvironment DOI Creative Commons

Elisa Cauli,

Michela Anna Polidoro, Simona Marzorati

et al.

Journal of Biological Engineering, Journal Year: 2023, Volume and Issue: 17(1)

Published: Aug. 17, 2023

Abstract The approval of anticancer therapeutic strategies is still slowed down by the lack models able to faithfully reproduce in vivo cancer physiology. On one hand, conventional vitro fail recapitulate organ and tissue structures, fluid flows, mechanical stimuli characterizing human body compartments. other animal cannot typical tumor microenvironment, essential study behavior progression. This reviews cancer-on-chips as most promising tools model investigate microenvironment metastasis. We also described how cancer-on-chip devices have been developed implemented common primary cancers their metastatic sites. Pros cons this technology are then discussed highlighting future challenges close gap between pre-clinical clinical studies accelerate new therapies humans.

Language: Английский

Citations

22
In Vitro Models for Peripheral Nerve Regeneration DOI

Jarin Tusnim,

Peter Kutuzov,

Jonathan M. Grasman

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 26, 2024

Abstract Peripheral nerve injury (PNI) resulting in lesions is highly prevalent clinically, but current therapeutic approaches fail to provide satisfactory outcomes many patients. While peripheral nerves have intrinsic regenerative capacity, the capabilities of are often insufficient restore full functionality. This highlights an unmet need for developing more effective strategies repair damaged and improve success. Consequently, researchers actively exploring a variety strategies, encompassing local delivery trophic factors or bioactive molecules, design advanced biomaterials that interact with regenerating axons, augmentation guidance conduits complex prostheses. However, clinical translation these technologies remains limited, emphasizing continued research on regeneration modalities can enhance functional restoration. Experimental models accurately recapitulate key aspects biology accelerate development by enabling systematic testing new techniques. Advancing therapies PNI requires bridging gap between basic science discoveries application. review discusses different vitro repair, including their advantages, limitations, potential applications.

Language: Английский

Citations

7
Step-by-step fabrication of heart-on-chip systems as models for cardiac disease modeling and drug screening DOI

Sara Deir,

Yasaman Mozhdehbakhsh Mofrad, Shohreh Mashayekhan

et al.

Talanta, Journal Year: 2023, Volume and Issue: 266, P. 124901 - 124901

Published: July 15, 2023

Language: Английский

Citations

12
Numerical evaluation and experimental validation of fluid flow behavior within an organ-on-a-chip model DOI Creative Commons
Violeta Carvalho, Inês M. Gonçalves, Nélson Rodrigues

et al.

Computer Methods and Programs in Biomedicine, Journal Year: 2023, Volume and Issue: 243, P. 107883 - 107883

Published: Oct. 28, 2023

By combining biomaterials, cell culture, and microfluidic technology, organ-on-a-chip (OoC) platforms have the ability to reproduce physiological microenvironment of human organs. For this reason, these advanced devices been used resemble various diseases investigate novel treatments. In addition experimental assessment, numerical studies biodevices performed aiming at their improvement optimization. Despite considerable progress in modeling biodevices, validation computational models through comparison with assays remains a significant gap current literature. This step is critical ensure accuracy reliability models, consequently enhance confidence predictive results. The aim present work develop model capable reproducing fluid flow behavior within an OoC, for future investigations, encompassing geometry optimization.In study, OoC device was undertaken. comprised both quantitative qualitative assessments trace microparticles flowing physical model. High-speed microscopy images flow, using blood analog fluid, were analyzed compared simulations run Ansys Fluent software. analysis, particles' paths inlet bifurcations observed whereas, particle velocities measured. Furthermore, oxygen transport simulated evaluated different Reynolds numbers.In analyses, results predicted by ones outputted good agreement. These findings underscore capability potential developed examination vertical positions organoid has revealed that lower positions, predominantly occurs diffusion, leading symmetric distribution oxygen. Contrastingly, convection phenomenon becomes more evident upper region organoid.The successful against data shows its simulating which can expedite design process reducing need prototypes' fabrication costly laboratory experiments.

Language: Английский

Citations

12
Biomimetic microfluidic chips for toxicity assessment of environmental pollutants DOI

Xin-yue Du,

Jin‐yan Yang

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 919, P. 170745 - 170745

Published: Feb. 8, 2024

Language: Английский

Citations

5
A Human Ovarian Tumor & Liver Organ-on-Chip for Simultaneous and More Predictive Toxo-Efficacy Assays DOI Creative Commons
Arianna Fedi, Chiara Vitale, Marco Fato

et al.

Bioengineering, Journal Year: 2023, Volume and Issue: 10(2), P. 270 - 270

Published: Feb. 18, 2023

In oncology, the poor success rate of clinical trials is becoming increasingly evident due to weak predictability preclinical assays, which either do not recapitulate complexity human tissues (i.e., in vitro tests) or reveal species-specific outcomes animal testing). Therefore, development novel approaches fundamental for better evaluating anti-cancer treatments. Here, a multicompartmental organ-on-chip (OOC) platform was adopted fluidically connect 3D ovarian cancer hepatic cellular models and resemble systemic cisplatin administration contemporarily investigating drug efficacy hepatotoxic effects physiological context. Computational fluid dynamics performed impose capillary-like blood flows predict diffusion. After concentration screening using 2D/3D tissue models, cytotoxicity assays were conducted OOC compared with static co-cultures dynamic single-organ models. A linear decay SKOV-3 HepG2 liver cell viability observed increasing concentration. Furthermore, showed higher resistance than 2D model conditions. Most importantly, when therapy, experimental approach combining culture, fluid-dynamic conditions, multi-organ connection displayed most predictive toxicity results, demonstrating that OOC-based are reliable 3Rs alternatives preclinic.

Language: Английский

Citations

11