TrAC Trends in Analytical Chemistry, Journal Year: 2023, Volume and Issue: 171, P. 117485 - 117485
Published: Dec. 16, 2023
Language: Английский
Biosensors, Journal Year: 2024, Volume and Issue: 14(5), P. 210 - 210
Published: April 23, 2024
The rapid and sensitive detection of pathogenic bacteria is becoming increasingly important for the timely prevention contamination treatment infections. Biosensors based on nucleic acid aptamers, integrated with optical, electrochemical, mass-sensitive analytical techniques, have garnered intense interest because their versatility, cost-efficiency, ability to exhibit high affinity specificity in binding bacterial biomarkers, toxins, whole cells. This review highlights development structural characterization, chemical modifications enabling optimized recognition properties enhanced stability complex biological matrices. Furthermore, recent examples aptasensors cells, toxins are discussed. Finally, we explore barriers discuss perspectives application aptamer-based detection.
Language: Английский
Citations
14
ACS Omega, Journal Year: 2024, Volume and Issue: 9(2), P. 2841 - 2849
Published: Jan. 4, 2024
In order to combat the complex and diverse infections caused by bacteria, it is essential develop efficient diagnostic tools. Current techniques for bacterial detection rely on laborious multistep procedures, with high costs extended time of analysis. To overcome these limitations, we propose here a novel portable electrochemical biosensor rapid identification Gram-positive bacteria that leverages recognition capabilities vancomycin aptamers. A vancomycin-modified screen-printed carbon electrode was used selectively capture susceptible this antibiotic. Electrochemical impedance spectroscopy scanning electron microscopy demonstrated achieved in 10 min, limit only 2 CFU/mL. We then tested device's potential aptamer-based using Staphylococcus aureus Bacillus cereus as test strains. Specifically, electrodes captured were exposed species-specific aptamers, resulting changes current intensity analyzed differential pulse voltammetry. When directly untreated milk or serum, system able successfully identify small amount S. B. (100 CFU/mL) less than 45 min. This has serve an invaluable tool could be used, even inexperienced staff, broad range settings including clinical diagnostics, food safety analysis, environmental monitoring, security applications.
Language: Английский
Citations
12
Talanta, Journal Year: 2024, Volume and Issue: 282, P. 127002 - 127002
Published: Oct. 9, 2024
Language: Английский
Citations
9
Foods, Journal Year: 2025, Volume and Issue: 14(3), P. 351 - 351
Published: Jan. 22, 2025
According to the scientific information reviewed, cheese is highly susceptible contamination by mycotoxin-producing fungi, primarily species from genera Aspergillus (A. niger, A. flavus) and Penicillium (P. commune, P. solitum, palitans, crustosum). Studies on various types of made cow’s milk report an average concentration Aflatoxin M1 (AFM1) at 13,000 ng kg−1, which alarming since regulatory limits for AFM1 in range 250 500 kg−1. For instance, set Codex Alimentarius, European Commission (EC), Turkey, Iran are In Netherlands, limit 200 Italy, it 450 However, mycotoxins frequently exceeds these limits, including critical such as ochratoxin A, citrinin, cyclopiazonic acid, pose significant global health concerns. Therefore, this study aims review mycobiota responsible producing key assess influence physicochemical factors fungal growth mycotoxin production. By incorporating control strategies hygiene practices, pasteurization, use preservatives, seeks improve methodologies production chain mitigate fungi mycotoxins.
Language: Английский
Citations
1
Heliyon, Journal Year: 2024, Volume and Issue: 10(5), P. e26988 - e26988
Published: Feb. 28, 2024
is a strain of
Language: Английский
Citations
6
The Microbe, Journal Year: 2024, Volume and Issue: 4, P. 100131 - 100131
Published: July 20, 2024
We present the review and meta-analysis of prevalence Staphylococcus aureus isolates from various food sources. PubMed, Google Scholar, Scopus, Science Direct were searched for articles 2012 to 2022. S. is a pathogenic bacterium in natural skin flora, that can cause variety diseases with different degrees severity. Although its habitat are humans animals, be found water, soil contaminated surfaces. Consequently, multiple routes involved contamination by aureus. The was most prevalent ready-to-eat (35.1 %), meat (21.7 %) dairy products (18.5 %). Among products, represented 59.51 % distributed as such: 44 beef meat, 28 pork 22 chicken 6 turkey meat. Antibiotic resistance studies showed penicillins common (61 while resistant quinolones, amphenicols rifamycins low (<10 Pooled antimicrobial isolated strains revealed 68 all carried at least one antibiotic clinical use. Future needed assess resistance, food-associated stress biofilm formation foodborne pathogen Furthermore, improved diagnostic tools implementation surveillance programs could lead reduction burden caused
Language: Английский
Citations
5
Journal of Pharmaceutical and Biomedical Analysis, Journal Year: 2025, Volume and Issue: 255, P. 116661 - 116661
Published: Jan. 5, 2025
Language: Английский
Citations
0
Food Frontiers, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 26, 2025
ABSTRACT The prevalence of foodborne outbreaks due to the consumption uncooked and ready‐to‐eat fruits vegetables has seen a noticeable increase, particularly in environments lacking sanitation. This article extensively explores recent advancements detection pathogens vegetables, alongside potential prevention strategies. Predominantly, like Listeria monocytogenes , Escherichia coli Salmonella enterica are main culprits linked these food items globally. Notably, contamination is more prevalent fresh leafy greens than fruit products. Various methods such as culturing, microscopy, immunological assays, polymerase chain reaction (PCR), biosensors, hyperspectral imaging have proven effective identifying foods. Nonetheless, come with challenges, including time consumption, accuracy concerns, high costs. Research ongoing refine techniques, efforts combining methodologies PCR–enzyme‐linked immunosorbent assay integrating culturing PCR. Additionally, several interventions, cold plasma treatment, ultraviolet irradiation, application edible coatings, shown promise mitigating risks, thereby enhancing safety produce items.
Language: Английский
Citations
0
Critical Reviews in Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 15
Published: March 18, 2025
Nowadays, aptamer-based biosensors and electrochemical measurements represent one of the efficient tools for detection drugs in both medical veterinary. Precise trace values analysis chemicals, especially drugs, plays a crucial role food therapeutic safety evaluations that are often time-consuming costly. Ultimately, accurate determination medications like antibiotics food, environmental resources, biological matrices is very important protecting public health drug monitoring (TDM) effective treatment. This review highlights recent advancements aptasensors as an innovative approach offering high sensitivity, specificity, rapid clinical veterinary at lower costs. We provide comprehensive overview discuss challenges prospects aptasensing residue across various samples.
Language: Английский
Citations
0
Applied Sciences, Journal Year: 2025, Volume and Issue: 15(7), P. 3745 - 3745
Published: March 28, 2025
The cleaning process kinetics of metallic surfaces has been studied by means digital video monitoring. Two theoretical models based on experimental observations are proposed for two possible mechanisms and practical cases that can be adapted to these analyzed. As a kinetic parameter, the duration surface is defined from time evolution color intensities their variance. These times allow us characterize optimize procedure. Apparent activation energies estimated characteristic at different temperatures.
Language: Английский
Citations
0