Bioimplant-on-a-Chip for Facile Investigation of Periodontal Ligament Formation on Biogenic Hydroxyapatite/Ti₆Al₄ V Implants

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: May 13, 2025

Highly osseointegrative dental implants surrounded by reconstructed periodontal tissues represent a promising strategy for functional tooth replacement, as they mimic the structural and physiological characteristics of natural teeth. However, there is currently lack in vitro platforms that can effectively evaluate integration engineered ligament (PDL) with bioimplants. In this study, we developed bioimplant-on-a-chip (BoC) platform designed to recapitulate native PDL-cementum interface assess early stage biological performance bioimplants vitro. The BoC consists implant, calcium phosphate cement (CPC) insert, nanopatterned polydimethylsiloxane (PDMS) substrate, PDL-like tissue derived from human pulp stem cells (DPSCs). To establish viable culture conditions within platform, surface coatings cell seeding densities were optimized support formation tissue. Nanogrooved substrates incorporated guide cellular alignment, which was assessed through orientation analysis. Collagen fiber organization matrix deposition further examined indicators ligamentous maturation. Cementogenic activity evaluated immunofluorescent staining cementum protein-1 (CEMP-1) response varying biogenic hydroxyapatite (bHA) contents results demonstrated successful reproduction material-dependent differences CEMP-1 expression. This provides modular reproducible tool comparative evaluation physiologically relevant setting may be useful advancing regenerative strategies implantology.

Language: Английский

Intestinal organoids in inflammatory bowel disease: advances, applications, and future directions

Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 13

Published: May 12, 2025

Inflammatory bowel disease (IBD), characterized by chronic gastrointestinal inflammation, is a significant global health challenge. Traditional models often fail to accurately reflect human pathophysiology, leading suboptimal treatments. This review provides an overview of recent advancements in intestinal organoid technology and its role IBD research. Organoids, derived from patient-specific or pluripotent stem cells, retain the genetic, epigenetic, structural characteristics native gut, allowing for precise modeling key aspects IBD. Innovations CRISPR editing, organoid-microbe co-cultures, organ-on-a-chip systems have enhanced physiological relevance these models, facilitating drug discovery personalized therapy screening. However, challenges such as vascularization deficits need standardized protocols remain. underscores interdisciplinary efforts bridge gap between complex reality Future directions include development scalable vascularized robust regulatory frameworks accelerate therapeutic translation. Organoids hold promise unraveling heterogeneity transforming management.

Language: Английский