The spread of microbiota in the air of an underground hard coal mine – A case study DOI Creative Commons
Piotr Cheluszka, Iwona Paśmionka, J. Gospodarek

et al.

Building and Environment, Journal Year: 2023, Volume and Issue: 242, P. 110495 - 110495

Published: June 5, 2023

The environment of underground hard coal mines, due to high humidity and temperature, combined with the presence organic substances very dust content, provides optimal conditions for growth development microorganisms, including pathogens. presented research was aimed at determining quantitative qualitative diversity air in mining excavations. Microbiological tests samples were carried out according standard microbiological techniques. study microbial communities made it possible determine unit concentrations identify potential pathogens, analyze manner their spread an mine. A large number variety microorganisms observed bioaerosol, pathogenic ones (e.g., Staphylococcus aureus, Aspergillus flavus) that may pose a threat humans by causing lung skin diseases. Due insufficient state global knowledge this area obtained results are helpful modernization existing designing new excavation ventilation systems, as well refreshing particularly hazardous places.

Language: Английский

Optimisation of Potato Dextrose Agar Culture Medium for Actinobacteria Growth DOI Creative Commons

Elian Chaves Ribeiro,

Emanuelle Ketthlen Nunes Araújo,

Margareth Santos Costa Penha

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(3), P. 654 - 654

Published: March 13, 2025

The objective of this study was to optimise the potato dextrose agar (PDA) culture medium in terms its potential for use growth actinobacteria. strain used a species actinobacteria previously identified as Streptosporangium sp. (P1C3), characterised by slow (20 days incubation), low aerial mycelium production, and no pigment production. To determine optimal formulation, (P1C3) tested incubation time across 27 formulations based on PDA medium. A central composite rotational design (CCRD) experimental methodology employed, where glucose concentration (g/L), yeast extract pH, temperature were tested. Among formulations, 01, 05, 09, 13 showed reduction complete growth, which linearly influenced four variables. Response surface analysis indicated that values promoting shortest 10 g/L concentration, from 1 3 pH levels between 5.7 7.2, temperatures 24 °C 32 °C. optimisation proved effective improving isolation enhancing production metabolites with antimicrobial activity.

Language: Английский

Citations

0
The Role of Actinomycetes in a Cleaner Environment DOI

Veilumuth Pattapulavar,

J. Godwin Christopher

Environmental science and engineering, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 33

Published: Jan. 1, 2025

Language: Английский

Citations

0
Exploring the diversity and antimicrobial potential of actinomycetes isolated from different environments in Saudi Arabia: a systematic review DOI Creative Commons

Noof Refat Helmi

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: March 26, 2025

The increasing prevalence of antimicrobial resistance (AMR) presents a significant global health challenge, underscoring the urgent need for novel agents. Actinomycetes, particularly Streptomyces species, are well known synthesizing bioactive compounds with antibacterial, antifungal, and antiviral properties. This review explores diversity potential actinomycetes from Saudi Arabia’s unique ecosystems, including terrestrial (soil, rhizosphere), aquatic (marine, freshwater), extreme (deserts, caves, hot springs, mountains, mangroves), other environments. adaptation these microorganisms to harsh environmental conditions has driven evolution strains enhanced biosynthetic capacities. Several studies have demonstrated their efficacy against multidrug-resistant pathogens, methicillin-resistant Staphylococcus aureus (MRSA), extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae, Pseudomonas aeruginosa , Candida albicans . However, challenges in actinomycete research persist, difficulties culturing rare strains, limited genomic characterization, high production costs. Recent advancements, such as genome mining, metagenomics, AI-driven bioinformatics, CRISPR-based gene activation, offer promising avenues unlocking compounds. Additionally, synthetic biology, advanced fermentation technologies, nanotechnology-based drug delivery systems enhancing industrial scalability actinomycete-derived antibiotics. Beyond antimicrobials, show applications oncology, immunotherapy, agriculture. Alternative therapeutic strategies, quorum sensing inhibitors, phage therapy, combination therapies, being explored combat AMR. Cutting-edge analytical techniques, mass spectrometry, liquid chromatography, nuclear magnetic resonance spectroscopy (NMR), essential structural elucidation mechanism characterization new To harness microbial biodiversity effectively, interdisciplinary collaborations between microbiologists, biotechnologists, pharmaceutical industries crucial. Sustainable bioprospecting bioprocessing strategies will facilitate translation into clinically viable therapeutics. Expanding efforts underexplored ecosystems can lead groundbreaking discoveries antibiotic development beyond.

Language: Английский

Citations

0
Uncovering Microbial Diversity and Community Structure of Black Spots Residing in Tomb Mural Painting DOI Creative Commons
Qiang Li, He Zhang, Zeng Wang

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(4), P. 755 - 755

Published: March 26, 2025

Microbes colonizing cultural artifacts are a ubiquitous phenomenon which may occur during burial, post-excavation, and storage periods, thereby seriously affecting sustainable heritage conservation. In this study, high-throughput sequencing technology was applied to analyze the microbial community structure in ancient mural paintings surrounding air, as well identify most characteristic taxa causing black spot contamination. The results showed that members of genera Gliomastix Ochroconis were highly abundant black-spots-contaminated areas rarely detected air uncontaminated paintings. Air samples two tombs no significant difference Chao1 Shannon indices, whereas statistically differences observed compared those collected from spots. taxonomic diversity soil-covered exhibited similar structures at genus level. Moreover, when other tombs, spots differed not only composition but also assembly processes co-occurrence patterns, such much less network complexity area. Functional predictions uncover presence functional profiles involved nitrogen cycling, organic matter degradation, animal human pathogens, representing potential threat relics public health. These advance our understanding impacts archeological excavations on variation tomb

Language: Английский

Citations

0
Harnessing the Potential of Sulphur Oxidizing Bacillus with Agrochemicals for Improving Soil Health and Nutrient Acquisition in Brassica Napus L DOI

Vishnu,

Poonam Sharma, Jupinder Kaur

et al.

Journal of soil science and plant nutrition, Journal Year: 2025, Volume and Issue: unknown

Published: April 2, 2025

Language: Английский

Citations

0
Diversity and bioactive potential of Actinomycetia from the rhizosphere soil of Juniperus excelsa DOI Open Access
Stepan Tistechok, Ivan Roman, Victor Fedorenko

et al.

Folia Microbiologica, Journal Year: 2023, Volume and Issue: 68(4), P. 645 - 653

Published: March 22, 2023

Language: Английский

Citations

8
Outdoor climate drives diversity patterns of dominant microbial taxa in caves worldwide DOI Creative Commons
Federico Biagioli, Claudia Coleine, Manuel Delgado‐Baquerizo

et al.

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 906, P. 167674 - 167674

Published: Oct. 7, 2023

The cave microbiota is assumed to be shaped by indoor microclimate, biotic and abiotic factors, which are largely dependent from outside environmental conditions; however, this knowledge available at local or regional scales only. To address gap, we reanalyzed over 1050 bacterial fungal communities of caves worldwide, found that outdoor temperature rainfall play a critical role in explaining differences microbial diversity patterns global caves, selecting specific dominant taxa across gradients growing aridity conditions with arid climate leading reduction total diversity. Moreover, (from 186 1908 taxa) 467 1619 increased under temperate-tropical temperate-continental climatic regions, respectively, highlighting an opposite preference for the two compartments. We hypothesized geographical, variables lithology epistatic drivers assembling their taxa, whose ecological responses could useful predict fate these subterranean environments context change. Our work elucidates intimate connection between surface ecosystems sensitivity changes degradation. This also provides natural benchmark biogeographic information globally protection strategies aiming conservation underground environments.

Language: Английский

Citations

8
The Natural Products Discovery Center: Release of the First 8490 Sequenced Strains for Exploring Actinobacteria Biosynthetic Diversity DOI Creative Commons
Edward Kalkreuter, Satria A. Kautsar, Dong Yang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 15, 2023

Actinobacteria, the bacterial phylum most renowned for natural product discovery, has been established as a valuable source drug discovery and biotechnology but is underrepresented within accessible genome strain collections. Herein, we introduce Natural Products Discovery Center (NPDC), featuring 122,449 strains assembled over eight decades, genomes of first 8490 NPDC (7142 Actinobacteria), online Portal making both publicly available. A comparative survey RefSeq Actinobacteria highlights taxonomic biosynthetic diversity collection, including three new genera, hundreds species, ~7000 gene cluster families. Selected examples demonstrate how Portal's metadata, genomes, clusters can be leveraged using mining approaches. Our findings underscore ongoing significance in serves an unparalleled resource genomes.

Language: Английский

Citations

7
Microbial communities in carbonate precipitates from drip waters in Nerja Cave, Spain DOI Creative Commons
Valme Jurado, Yolanda del Rosal Padial, C. Jiménez de Cisneros

et al.

PeerJ, Journal Year: 2022, Volume and Issue: 10, P. e13399 - e13399

Published: May 3, 2022

Research on cave microorganisms has mainly focused the microbial communities thriving speleothems, rocks and sediments; however, drip water bacteria calcite precipitation received less attention. In this study, of carbonate precipitates from waters in Nerja, a show close to sea southeastern Spain, were investigated. We observed pronounced difference bacterial composition precipitates, depending galleries halls. The most abundant phylum halls entrance was Proteobacteria , due low depth sector, direct influence garden top soil infiltration into cave, as well abundance members order Hyphomicrobiales dispersing plant roots, other Betaproteobacteria Gammaproteobacteria common inhabitants. marine aerosols explained presence Marinobacter, Idiomarina, Thalassobaculum, Altererythrobacter short distance sea. Nineteen out forty six genera identified have been reported precipitate likely role mineral deposition.

Language: Английский

Citations

11
Catechol-type siderophore producer Streptomyces spp. with quorum-quenching activity isolated from wild medicinal plants rhizosphere DOI Creative Commons

Fatemeh Alinejad,

Gholam Khodakaramian, Darshan Chandramowli

et al.

The Microbe, Journal Year: 2024, Volume and Issue: 2, P. 100033 - 100033

Published: Jan. 2, 2024

Traditional medicinal plants represent a unique source for the isolation of Streptomycetes and antimicrobial compounds. Antimicrobial activity Streptomyces isolates collected from rhizosphere different native in Iran, was investigated against Gram-positive Gram-negative bacteria. Multi-omics analysis performed to identify active compounds corresponding observed bioactivities. Since cell-to-cell communication mediated by N-acyl homoserine lactones (AHLs) is important virulence pathogenic bacteria, quorum quenching their ability degrade C6-HSL C8-HSL were tested. Two highly related derived Helichrysum rubicundum Rumex acetosa rhizosphere, respectively, exhibited bioactivity indicator Metabolite extracts obtained liquid cultures both revealed production catechol-peptide siderophores streptobactin tribenarthin. Proteomic confirmed presence proteins encoded lidamycin biosynthetic gene clusters. Quorum strains AHL-degrading enzyme using GFP producing biosensor LC-MS analysis. This study demonstrates catecholate siderophore QQ potential two Mishan Plain, Hamedan Province, Iran metabolomics proteomics Production terrestrial sp. first time.

Language: Английский

Citations

2