Advances in Genetic Engineering in Improving Photosynthesis and Microalgal Productivity

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(3), P. 1898 - 1898

Published: Jan. 18, 2023

Even though sunlight energy far outweighs the required by human activities, its utilization is a key goal in field of renewable energies. Microalgae have emerged as promising new and sustainable feedstock for meeting rising food feed demand. Because traditional methods microalgal improvement are likely to reached their limits, genetic engineering expected allow further increases photosynthesis productivity microalgae. Understanding mechanisms that control will enable researchers identify targets and, end, increase biomass yield, offsetting costs cultivation systems downstream processing. This review describes molecular events happen during through discusses future strategies limitations productivity. We highlight major achievements manipulating fundamental production, well approaches making significant contributions upcoming microalgal-based biotechnology.

Language: Английский

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Advances in Chlorella Microalgae for Sustainable Wastewater Treatment and Bioproduction

Chemical Engineering Journal Advances, Journal Year: 2025, Volume and Issue: unknown, P. 100715 - 100715

Published: Feb. 1, 2025

Language: Английский

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Green strategies for enhanced microalgae processes: leveraging bio-derived adsorbents, green solvents, and synthetic biology

Journal of environmental chemical engineering, Journal Year: 2025, Volume and Issue: 13(2), P. 115986 - 115986

Published: Feb. 26, 2025

Language: Английский

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Context‐dependent antisense transcription from a neighboring gene interferes with the expression of mNeonGreen as a functional in vivo fluorescent reporter in the chloroplast of Chlamydomonas reinhardtii

The Plant Journal, Journal Year: 2024, Volume and Issue: 119(5), P. 2255 - 2272

Published: July 17, 2024

Advancing chloroplast genetic engineering in Chlamydomonas reinhardtii remains challenging, decades after its first successful transformation. This study introduces the development of a chloroplast-optimized mNeonGreen fluorescent reporter, enabling vivo observation through sixfold increase fluorescence via context-aware construct engineering. Our research highlights influence transcriptional readthrough and antisense mRNA pairing on post-transcriptional regulation, pointing to novel strategies for optimizing heterologous gene expression. We further demonstrate applicability these insights using an accessible experimentation system glass-bead transformation reestablishment photosynthesis psbH mutants, focusing mitigation effects. By characterizing expression regulatory elements such as PrrnS, 5'atpA, 3' rbcL sense-transcriptional context, we documented up twofold improvement levels. findings contribute new tools molecular biology evidence fundamental regulation processes that could enable more effective strategies. work not only paves way efficient chloroplasts but also deepens our understanding mechanisms at play.

Language: Английский

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Antisense transcription from a neighboring gene interferes with the expression of mNeonGreen as a functionalin vivofluorescent reporter in the chloroplast ofChlamydomonas reinhardtii

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 12, 2023

ABSTRACT Although over 30 years have passed since Chlamydomonas reinhardtii chloroplast transformation was first achieved, robust genetic engineering of the still remains a challenging task. The glass-bead method has enabled simple and accessible C. TN72 strain, allowing generation marker-free transplastomic strains for low-cost experimentation. However, lack functional in vivo fluorescent reporters limit research widespread development engineering. Here, we developed codon-optimised mNeonGreen reporter, which can be detected through fluorescence microscopy fluorometry, by context-aware construct engineering, leading up to ∼6-fold increase fluorescence. We found evidence post-transcriptional regulation gene expression derived from formation antisense pairing mRNAs due transcriptional readthrough convergent adjacent gene, validated detection double-stranded RNA. In addition, approaches were used modulate readthrough, better understanding context effects that are relevant heterologous expression. Finally, characterised suite regulatory parts transgene sense-transcriptional regarding selectable marker, achieving ∼2-fold levels control, with use P rrnS, 5’ atpA 3’ rbcL endogenous sequences . This work provides new tools studying basic aspects molecular biology , as well fundamental processes may enable developing rules more efficient

Language: Английский

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Advances in light system engineering across the phototrophic spectrum

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 12, 2024

Current work in photosynthetic engineering is progressing along the lines of cyanobacterial, microalgal, and plant research. These are interconnected through fundamental mechanisms photosynthesis advances one field can often be leveraged to improve another. It worthwhile for researchers specializing or more these systems aware being done across entire research space as parallel techniques experimental approaches applied This review focuses on published recent years related light reactions cyanobacteria, eukaryotic algae, plants. Highlighted attempts efficiency, subsequent biomass production. Also discussed studies cross-field heterologous expression, augmented novel capture systems. reviewed context translatability diverse organisms.

Language: Английский

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