Delivery mechanism can enhance probiotic activity against honey bee pathogens

The ISME Journal, Journal Year: 2023, Volume and Issue: 17(9), P. 1382 - 1395

Published: June 14, 2023

Managed honey bee (Apis mellifera) populations play a crucial role in supporting pollination of food crops but are facing unsustainable colony losses, largely due to rampant disease spread within agricultural environments. While mounting evidence suggests that select lactobacilli strains (some being natural symbionts bees) can protect against multiple infections, there has been limited validation at the field-level and few methods exist for applying viable microorganisms hive. Here, we compare how two different delivery systems-standard pollen patty infusion novel spray-based formulation-affect supplementation three-strain consortium (LX3). Hives pathogen-dense region California supplemented 4 weeks then monitored over 20-week period health outcomes. Results show both facilitate uptake LX3 adult bees, although do not colonize long-term. Despite this, treatments induce transcriptional immune responses leading sustained decreases many opportunistic bacterial fungal pathogens, as well selective enrichment core including Bombilactobacillus, Bifidobacterium, Lactobacillus, Bartonella spp. These changes ultimately associated with greater brood production growth relative vehicle controls, no apparent trade-offs ectoparasitic Varroa mite burdens. Furthermore, spray-LX3 exerts potent activities Ascosphaera apis (a deadly pathogen) likely stemming from in-hive dispersal differences, whereas patty-LX3 promotes synergistic development via unique nutritional benefits. findings provide foundational basis probiotic application apiculture collectively highlight importance considering method management strategies.

Language: Английский

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Prospects of probiotics in beekeeping: a review for sustainable approach to boost honeybee health

Archives of Microbiology, Journal Year: 2024, Volume and Issue: 206(5)

Published: April 4, 2024

Language: Английский

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Isolation and probiotic evaluation of Apilactobacillus kunkeei and Bombella sp. from Apis mellifera anatoliaca and Bombus terrestris

International Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 4, 2025

Language: Английский

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Gut microbiota influences onset of foraging-related behavior but not physiological hallmarks of division of labor in honeybees

mBio, Journal Year: 2024, Volume and Issue: 15(9)

Published: July 29, 2024

ABSTRACT Gut microbes can impact cognition and behavior, but whether they regulate the division of labor in animal societies is unknown. We addressed this question using honeybees since exhibit between nurses foragers because their gut microbiota be manipulated. Using automated behavioral tracking controlling for co-housing effects, we show that influence age at which bees start expressing foraging-like behaviors laboratory have no effects on time spent a foraging arena number trips. Moreover, did not hallmarks maturation such as body weight, cuticular hydrocarbon profile, hypopharyngeal gland size, gene expression, proportion maturing into foragers. Overall, study shows honeybee plays role onset foraging-related behavior without permanent consequences colony-level several physiological maturation. IMPORTANCE The emerging model system studying microbiota-host interactions. Previous studies reported multiple worker bee phenotypes, all change during maturation—the transition from nursing to foraging. tested documented may stem an effect only subtly affected maturation: it accelerated affecting overall or average output. also found host (CHC) expression maturation-related genes. These results are inconsistent with previous reporting weight CHC profile. Our experiments revealed co-housed tend converge physiology, suggesting spurious associations emerge when rearing environments replicated sufficiently accounted analytically.

Language: Английский

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Distinct Communities and Differing Dispersal Routes in Bacteria and Fungi of Honey Bees, Honey, and Flowers

Microbial Ecology, Journal Year: 2024, Volume and Issue: 87(1)

Published: July 30, 2024

Abstract Microbiota, the communities of microbes on and in organisms or organic matter, are essential for functioning ecosystems. How shared transmitted delineates formation a microbiota. As pollinators forage, they offer route to transfer among flowering plants, themselves, their nests. To assess how two components microbiota, bacteria fungi, pollination transferred, we focused honey bee Apis mellifera collected bee, (representing hive microbiota), flower samples three times during summer Finland. We identified fungi by DNA metabarcoding. determine impact bees’ choices also plant honey. The bacterial bees, honey, flowers all differ greatly from each other, while fungal bees very similar, yet different flowers. time sampling area influence these For flowers, identity impacts both communities’ composition most. dispersal pathways acquired directly indirectly through Overall, distinctiveness microbiota surrounding suggests sharing them occurs but plays minor role established

Language: Английский

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The neonicotinoid acetamiprid reduces larval and adult survival in honeybees (Apis mellifera) and interacts with a fungicide mixture

Environmental Pollution, Journal Year: 2024, Volume and Issue: 360, P. 124643 - 124643

Published: Aug. 2, 2024

Plant protection products (PPPs), which are frequently used in agriculture, can be major stressors for honeybees. They have been found abundantly the beehive, particularly pollen. Few studies analysed effects on honeybee larvae, and little is known about of insecticide-fungicide-mixtures, although this a highly realistic exposure scenario. We asked whether combination insecticide fungicides would affect developing bees. Honeybee larvae (Apis mellifera carnica) were reared vitro larval diets containing different PPPs at two concentrations, derived from residues neonicotinoid acetamiprid, combined boscalid/dimoxystrobin mixture all three substances. Mortality was assessed larval, pupal, adult stages, size weight newly emerged bees measured. The treatment higher concentrations significantly reduced survival. Interestingly, survival not affected by high concentrated insecticide-fungicides-mixture. However, negative synergistic caused low insecticide-fungicides-mixture, had no effect when applied alone. lower led to lighter bees, unaffected. Our results suggest that environmental relevant harmful To fully understand interaction PPPs, more combinations should studied social solitary with possibly sensitivities.

Language: Английский

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Investigating Aerobic Hive Microflora: Role of Surface Microbiome of Apis Mellifera

Biology, Journal Year: 2025, Volume and Issue: 14(1), P. 88 - 88

Published: Jan. 17, 2025

This study investigated the surface microbiome of honeybee (Apis mellifera), focusing on diversity and functional roles its associated microbial communities. While significance to insect health behavior is increasingly recognized, research invertebrate microbiota lags behind that vertebrates. A combined metagenomic cultivation-based approach was employed characterize bacterial communities inhabiting exoskeleton. Our findings reveal a complex diverse exhibiting significant spatial environmental heterogeneity. The identification antimicrobial compound producers, validated through both culture analyses, including potentially novel Actinobacteria species, underscores potential impact these health, behavior, hive dynamics. contributes more profound ecological understanding microbiome, particularly in winter configuration.

Language: Английский

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Bee Microbiomes: Unveiling Diversity and Social Dynamics in Managed and Wild Bees

Published: Jan. 1, 2025

Language: Английский

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Antimicrobial-Resistant Environmental Bacteria Isolated Using a Network of Honey Bee Colonies (Apis mellifera L. 1758)

Transboundary and Emerging Diseases, Journal Year: 2023, Volume and Issue: 2023, P. 1 - 10

Published: Nov. 27, 2023

The phenomenon of antibiotic resistance stands as a paramount health challenge in the contemporary era. Within One Health approach, it becomes crucial to effectively track dissemination resistance, not only within humans and animals but also environment. To investigate environment, honey bee (Apis mellifera) has emerged prominent environmental bioindicator due its social, behavioral, morphological features. objective this study was describe antimicrobial (AMR) patterns bacterial isolates from body surface gut bees sampled 33 colonies throughout Emilia-Romagna region (Italy). A total 608 strains were examined for 19 distinct compounds various classes, results showed that more than 50% eight out nine provinces characteristics nonsusceptibility toward amoxicillin penicillin, and, generally, 98.19% isolated considered AMR 74.67% exhibited multidrug (MDR) characteristics, frequent Gram-negative (87.74%) Gram-positive ones (60.34%). Additionally, significant correlation with lower prevalence MDR bacteria demonstrated one province (Ferrara, odds ratio (OR) = 3.33, (1.67; 6.64), $p=0.0006$ ). In conclusion, provides evidence utility A. mellifera bioindicators bacteria, enabling their characterization distribution at geographical level. Additional investigations are required further explore potential role antimicrobial-resistant particularly terms association characteristics.

Language: Английский

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The Bee Gut Microbiota: Bridging Infective Agents Potential in the One Health Context

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(7), P. 3739 - 3739

Published: March 27, 2024

The bee gut microbiota plays an important role in the services bees pay to environment, humans and animals. Alongside, gut-associated microorganisms are vehiculated between apparently remote habitats, promoting microbial heterogeneity of visited microcosms transfer genetic elements. To date, no metaproteomics studies dealing with functional available. Here, we employ a approach explore fraction bacterial, fungal, unicellular parasites inhabiting gut. bacterial community portrays dynamic composition, accounting for specimens human animal concern. Their features highlight vehiculation virulence antimicrobial resistance traits. fungal parasite fractions include environment- animal-related specimens, whose metabolic activities support spatial spreading features. Host proteome depicts major physiological activities, supporting strategy simultaneous study multiple their host-crosstalks. Altogether, present provides better definition structure function microbiota, highlighting its impact variety strategies aimed at improving/overcoming several current hot topic issues such as resistance, environmental pollution promotion health.

Language: Английский

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