Diversity and Ecological Relevance of Fumarate‐Adding Enzymes in Oil Reservoir Microbial Communities

Environmental Microbiology, Journal Year: 2025, Volume and Issue: 27(3)

Published: March 1, 2025

ABSTRACT Oil reservoirs are important hydrocarbon‐rich environments, where the addition of hydrocarbons to fumarate mediated by fumarate‐adding enzymes (FAE) is one dominant mechanisms for anaerobic degradation hydrocarbons. However, currently available information about FaeA, catalytic subunit FAE, in situ petroleum limited. Here, we investigated diversity FaeA and FaeA‐harbouring microbes oil compared them with marine sediments. We obtained 67 clusters 46 MAGs from reservoirs. Most sequences all FaeA‐containing were endemic unique. In reservoirs, affiliated Archaeoglobus 13 bacterial phyla. Fermentative metabolism was a common lifestyle amongst these organisms. Genomes assigned Desulfobacterota, Caldatribacteriota Firmicutes_B most diverse prevalent, while Desulfobacterota Chloroflexota marine. Microbial community at phylum level strongly related but not This suggested that ability hydrocarbon biodegradation may shape structure Together, this study provided systematic comprehensive regarding high degraders underlined difference between environments

Language: Английский

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Development of Microbial Consortium and Its Influencing Factors for Enhanced Oil Recovery after Polymer Flooding: A Review

Processes, Journal Year: 2023, Volume and Issue: 11(10), P. 2853 - 2853

Published: Sept. 27, 2023

After polymer flooding, substantial oil and residual polymers remain in reservoirs, leading to plugging reduced recovery. MEOR (Microbial Enhanced Oil Recovery) aims release trapped by utilizing microorganisms their byproducts. The can use HPAM (hydrolyzed polyacrylamide) as an energy source for degradation, addressing reservoir issues improving However, are sensitive environmental conditions. This paper presents a detailed update of MEOR, including microbial products, mechanisms, merits demerits. effect the displacement fluid conditions on is thoroughly demonstrated elucidate influencing mechanism. Among these factors, crosslinkers, which have significant biological toxicity, affect efficiency MEOR. Limited research exists chemicals microorganisms’ properties, metabolism, mechanisms. development consortium, metabolic interaction, microprocesses also discussed. In addition, prior studies lack insights into interaction mechanisms using chemicals. Finally, field trials exist examine consortium’s introduce new technologies. review mainly explores factors microorganisms, confirms credibility after providing scientific basis theory

Language: Английский

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Mikroorganizmalar ve Bakteri Çeşitliliği

Özgür Yayınları eBooks, Journal Year: 2023, Volume and Issue: unknown

Published: March 24, 2023

Türk Dil Kurumu Veteriner Hekimliği Terimleri Sözlüğüne göre mikroorganizma; bakteri, mantar, protozoa ve mikroskobik algleri içeren canlılardır. Mikroorganizmaların bazı etkileri kullanım alanları konusunda asırlardır çeşitli bilgiler olmasına rağmen, ilk olarak 1675 yılında Leewenhoek’un mikroskobu keşfiyle mikroorganizmalar görülebilmiş üzerlerinde çalışmaya başlanmıştır. Ortalama 200 sene içerisinde Louis Pasteur fermantasyon aşaması ile ilgili çalışmalarında şarap yapımlarında istenilir olmayan tatların ortaya çıkışında mikroorganizmaların işlevlerini ispatlamış ısıl işlem uygulandığında istenmeyen ölümünde etki bıraktığı sonucuna ulaşmıştır. Pastörizasyon isimlendirilen bu uygulama günümüzde halen daha kabul görmektedir. O zamanlarda Robert Koch’un çalışmalarının da mikrobiyoloji alanına önemli katkıları olmuş alandaki çalışmaları günümüze kadar hızlı bir gelişme devam etmiştir. Mikroorganizmalar bakterilerin çeşitlilik özelliklerinin anlaşılması bakımından temel bilgileri içermektedir.

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Metagenomic and Culture-Based Analyses of Microbial Communities from Petroleum Reservoirs with High-Salinity Formation Water, and Their Biotechnological Potential

Biology, Journal Year: 2023, Volume and Issue: 12(10), P. 1300 - 1300

Published: Oct. 2, 2023

The reserves of light conditional oil in reservoirs with low-salinity formation water are decreasing worldwide, necessitating the extraction heavy from petroleum high-salinity water. As first stage defining microbial-enhanced recovery (MEOR) strategies for depleted reservoirs, microbial community composition was studied located Tatarstan (Russia) using metagenomic and culture-based approaches. Bacteria phyla Desulfobacterota, Halanaerobiaeota, Sinergistota, Pseudomonadota, Bacillota were revealed 16S rRNA-based high-throughput sequencing halophilic communities. Sulfidogenic bacteria predominated fields. 75 metagenome-assembled genomes (MAGs) prokaryotes reconstructed samples assigned to 16 bacterial phyla, including Bacillota, Thermotogota, Actinobacteriota, Spirochaetota, Patescibacteria, archaea phylum Halobacteriota (genus Methanohalophilus). Results analyses supported by isolation 20 pure cultures genera Desulfoplanes, Halanaerobium, Geotoga, Sphaerochaeta, Tangfeifania, Bacillus. isolated fermentative produced oil-displacing metabolites (lower fatty acids, alcohols, gases) sugar-containing proteinaceous substrates, which testify their potential MEOR. However, organic substrates stimulated growth sulfidogenic bacteria, addition fermenters. Methods enhanced should therefore be developed, combining production compounds suppression sulfidogenesis.

Language: Английский

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Effects of nutrient injection on the Xinjiang oil field microbial community studied in a long core flooding simulation device

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Oct. 12, 2023

Microbial Enhanced Oil Recovery (MEOR) is an option for recovering oil from depleted reservoirs. Numerous field trials of MEOR have confirmed distinct microbial community structure in diverse production wells within the same block. The variance reservoir communities, however, remains ambiguously documented. In this study, 8 m long core flooding simulation device was built on a laboratory scale to study dynamic changes indigenous Qizhong Block, Xinjiang field. During MEOR, there approximate 34% upswing extraction. Based 16S rRNA gene high-throughput sequencing, our results indicated that nutrition one factors affecting communities After introduction nutrients, hydrocarbon oxidizing bacteria became active, followed by sequential activation facultative anaerobes and anaerobic fermenting bacteria. This consistent with hypothesized succession ecological "food chain" reservoir, which preliminarily supported two-step theory microbes transitioning aerobic states. Furthermore, metagenomic microorganisms had potential functions degradation, gas surfactant production. Understanding improving recovery are both aided work.

Language: Английский

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Effects of waterway regulation structures on the planktonic community in the upper Yangtze River

Ecological Indicators, Journal Year: 2023, Volume and Issue: 155, P. 111049 - 111049

Published: Oct. 7, 2023

To investigate the effects of waterway regulation structures on hydraulic characteristics, water physicochemical factors, and planktonic community in upper Yangtze River, this study was conducted August September 2022 two ecological dikes Chongqing section River: longitudinal dike spur dike. High-throughput sequencing technology used to explore differences upstream downstream during dry season, as well their main influencing factors. The results showed that dissolved oxygen increased significantly, turbidity flow rate decreased significantly dikes. exhibited more pronounced changes quality hydrodynamic factors compared diversity index phytoplankton, zooplankton, fungi were higher than those upstream, bacterial eukaryotes at each sampling site. impact biodiversity greater dike, it alters velocity, turbidity, oxygen. major phyla bacteria, including Cryptophyta (70.37–80.57 %), Mollusca (7.56–37.50 Proteobacteria (48.53–55.92 had a relative abundance In contrast, phylum fungi, Ascomycota (37.15–56.85 an opposite trend both dike-to-dike comparison upstream-to-downstream above three plankton. analysis neutral model indicated predominance stochastic assembly processes demonstrated rates migration speciation Flow rate, oxygen, significance structure aquatic environment around Differences dike-related environmental result interactions among prokaryotes while complex network relationships These findings can provide theoretical basis for protection construction waterways engineering area.

Language: Английский

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Construction and application of petroleum-degrading bacterial agents: Community composition, lyophilization technology, and degradation mechanism

Journal of environmental chemical engineering, Journal Year: 2024, Volume and Issue: 12(6), P. 114904 - 114904

Published: Nov. 22, 2024

Language: Английский

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Kimya Eğitimine Teknolojinin Entegrasyonu

Özgür Yayınları eBooks, Journal Year: 2023, Volume and Issue: unknown

Published: March 24, 2023

Kimya bilimi soyut ve somut kavramları bir arada bulunduran, yaparak yaşayarak öğrenme ilkesine dayanarak öğrencilerin laboratuvar ortamında deney kalıcı öğrenmenin sağlandığı bilim dalıdır. Günümüzde birçok okulda olmaması, öğretmenlerin müfredatı yetiştirme endişesi ile deneylere yer verememesi veya kimyasalların pahalı olması gibi nedenden dolayı öğrenciler yapamamakta, uygulamalı anlatım gereken bazı konular öğretmen merkezli düz yöntemi işlenmektedir. Teknolojinin eğitim dünyasında yerini alması birlikte amaçlı hazırlanan web 2.0 araçları, animasyonlar, etkileşimli sanal laboratuvarlar yapay zekâ uygulamaları kimya kavramlarının somutlaştırılması konusunda kullandığımız ders materyallerinde biri haline gelmiştir. Farklı kullanım amacına yönelik araçları konusunun teorisi görsel materyallerle zenginleştirilerek öğrenci paylaşılabileceği aynı aracı konu içeriğine uygun oyun hazırlanarak sağlanması amaçlanabilir, zeka kullanan araçlar vasıtası ortamına taşınamayan deneyler ortamda olarak öğrenciye sunulabilir bu sayede dikkat çekme, keşfetme, açıklama, derinleştirme değerlendirme olmak üzere konunun anlatılmasında verdiğimiz tüm aşamalarda teknolojiden yararlanabiliriz. Bu doğrultuda araçlarını tanımak, ihtiyaca göre anlatımına entegre edebilecek yöntemleri bilmek ancak eğitime teknolojinin entegrasyonu mümkün olmaktadır. bölümde araçlarının eğitiminde kullanılmasının yanı sıra teknoloji entegrasyon yöntemlerinden konularının öğretiminde kullanılabilecek tabanlı harmanlanmış türlerinden ters yüz sınıf modeli anlatılmıştır.

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Nutrient Availability Contributes to Structural and Functional Diversity of Microbiome in Xinjiang Oilfield

Published: Jan. 1, 2024

Download This Paper Open PDF in Browser Add to My Library Share: Permalink Using these links will ensure access this page indefinitely Copy URL DOI

Language: Английский

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Horizontal and Vertical Comparison of Microbial Community Structures in a Low Permeability Reservoir at the Local Scale

Microorganisms, Journal Year: 2023, Volume and Issue: 11(12), P. 2862 - 2862

Published: Nov. 26, 2023

Petroleum microorganisms play a crucial role in the application of microbial-enhanced oil recovery, and community structures petroleum have been widely studied. Due to variations reservoir geological conditions, microbial communities exhibit unique characteristics. However, previous studies primarily focused on changes within single well, block, before after water flooding, thus, cross-horizon cross-regional comparative situ are lacking. In this study, 16S rRNA full-length sequencing method was adopted study bacterial crude samples taken from two wells at same depths (depths 2425 m 2412 m) but approximately 20 km apart Hujianshan oilfield, located Ordos Basin. At time, results were combined with another layer research data article (from depth 2140 m). The aim compare differences between horizontal scale vertical scale. revealed that there minimal influenced by distances small range (<20 km), while observed larger spatial dominant bacteria (Proteobacteria Bacteroidetes) different oilfields similar. Vertical (>300 had significant impacts communities, mainly controlled temperature. greater depth, higher formation temperature, leading an increase thermophilic anaerobic community.

Language: Английский

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