Food Control, Journal Year: 2024, Volume and Issue: unknown, P. 111127 - 111127
Published: Dec. 1, 2024
Language: Английский
EFSA Journal, Journal Year: 2025, Volume and Issue: 23(4)
Published: April 1, 2025
Abstract Carbapenemase‐producing Enterobacterales (CPE) have been reported in the food chain 14 out of 30 EU/EFTA countries. Commonly genes are blaVIM‐1, blaOXA‐48 and blaOXA‐181, followed by blaNDM‐5 blaIMI‐1. Escherichia coli, target most studies, Enterobacter cloacae complex, Klebsiella pneumoniae complex Salmonella Infantis frequent CPE. E. coli isolates show a high clonal diversity. IncHI2 (blaVIM‐1 blaOXA‐162), IncC blaNDM‐1), IncX3 (blaNDM‐5 blaOXA‐181), IncI IncL (blaOXA‐48) plasmids frequently reported. Most reports from terrestrial food‐producing animals their environments – mainly pigs, bovines poultry with occasional meat thereof (targets EU monitoring follow up trace back investigations). Few studies investigated foods aquatic animal origin non‐animal origin, finding great CPE A notable increase number detections has observed, predominantly surge certain countries 2021 (blaOXA‐181, Italy) 2023 (blaOXA‐48, Spain; blaOXA‐48, blaOXA‐244 blaNDM‐5, Portugal). Very few data points to circumstantial evidence transmission, and/or horizontal gene spread within from/to humans. Various methods used detect characterise chain. Improvement sensitivity should be investigated. Ten specific contingency plans for control, being epidemiological investigations (e.g. trace‐back) common action included those plans. Overall, remain scarce bacterial species sources beyond systematically monitored. Recommendations fill gaps on other sources, dissemination pathways optimisation detection given. One Health approach address drivers is needed.
Language: Английский
Citations
1
Annals of Clinical Microbiology and Antimicrobials, Journal Year: 2024, Volume and Issue: 23(1)
Published: June 17, 2024
Abstract Background The global dissemination of critical-priority carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKp) via food sources represents a significant public health concern. Epidemiological data on CR-hvKp in oysters Egypt is limited. This study aimed to investigate the potential role sold as source for K. (CRKP), (hvKp), and assess associated zoonotic risks. Methods A sample 330 fresh was randomly purchased from various retail fish markets divided into 33 pools. Bacteriological examination identification were performed. Carbapenem resistance isolates determined by phenotypic molecular methods. Additionally, presence identified based virulence gene markers ( peg-344 , rmpA rmpA2 iucA iroB ), followed string test. clustering strains carried out using R with pheatmap package. Results overall prevalence 48.5% (16 33), 13 displaying carbapenem resistance, one intermediate two sensitive. Both carbapenem-intermediate-resistant exhibited carbapenemase production, predominantly linked bla VIM (68.8%). HvKp at rate 62.5% (10/16); notably, most prevalent gene. Significantly, 10 CRKP possessed hypervirulence genes, contributing emergence CR-hvKp. Moreover, cluster analysis revealed same market. Conclusion provides first insight among Egypt. It underscores disseminating within aquatic ecosystems, presenting possible threat health.
Language: Английский
Citations
4
Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)
Published: Nov. 6, 2024
Abstract The emergence of critical-priority E. coli , carrying a wide array resistance and virulence factors through food sources, poses significant challenge to public health. This study aimed investigate the potential role oysters sold in Egypt as source for identify their virulence-associated gene profiles, assess associated zoonotic risks. A total 33 pooled fresh oyster samples were obtained from various retail fish markets examined bacteriologically presence . Antimicrobial was performed by disk-diffusion method, multiple antibiotic index (MAR) calculated. All isolates screened extended-spectrum beta-lactamase (ESBL) ( bla TEM SHV CTX−M, OXA−1 ), plasmid-mediated AmpC CMY−2 carbapenemases KPC NDM VIM OXA−48 ) genes Polymerase chain reaction. Moreover, virulence-encoding investigated. virulent MDR strains clustered using R with pheatmap package. prevalence 72.7% (24 out 33), 66.7% classified multi-drug resistant, 75% exhibited MAR values exceeding 0.2 threshold. Different antimicrobial sensitivity phenotypes genotype profiles identified isolates. most prevalent detected among all (22/24, 91.7%). Notably, non-ESBL producers positive CMY2 Carbapenem-resistant carbapenem-intermediate carbapenemase producers, predominance (11/24, 45.8%). Remarkably, twelve sixteen identified, papC (21/24, 87.5%) sfa (16/24, 66.7%) being prevalent. Most carry primarily extra-intestinal pathogenic (ExPEC) (87.5%) enteropathogenic (EPEC) (70.8%) pathotypes. Four exhibit cluster patterns. provides first insight into Egypt. It underscores disseminating these within aquatic ecosystems, presenting possible threat
Language: Английский
Citations
4
Microorganisms, Journal Year: 2023, Volume and Issue: 11(9), P. 2218 - 2218
Published: Aug. 31, 2023
Bivalve molluscan shellfish have been consumed for centuries. Being filter feeders, they may bioaccumulate some microorganisms present in coastal water, either naturally or through the discharge of human animal sewage. Despite regulations set up to avoid microbiological contamination shellfish, outbreaks still occur. After providing an overview showing their implication disease, this review aims highlight diversity bacteria enteric viruses detected species, including emerging pathogens. a critical discussion available methods and limitations, we address interest technological developments using genomics anticipate emergence In coming years, further research needs be performed need developed order design future surveillance help risk assessment studies, with ultimate objective protecting consumers enhancing microbial safety bivalve as healthy food.
Language: Английский
Citations
10
BMC Infectious Diseases, Journal Year: 2025, Volume and Issue: 25(1)
Published: Jan. 20, 2025
Language: Английский
Citations
0
Foods, Journal Year: 2025, Volume and Issue: 14(9), P. 1461 - 1461
Published: April 23, 2025
Seafood plays a vital role in human diets worldwide, serving as an important source of high-quality protein, omega-3 fatty acids, and essential vitamins minerals that promote health prevent various chronic conditions. The benefits seafood consumption are well documented, including reduced risk cardiovascular diseases, improved cognitive function, anti-inflammatory effects. However, the safety is compromised by multiple hazards can pose significant risks. Pathogenic microorganisms, bacteria, viruses, parasites, addition to microbial metabolites, prominent causes foodborne diseases linked consumption, necessitating reliable detection monitoring systems. Molecular biology digital techniques have emerged tools for rapid accurate identification these pathogens, enhancing protocols. Additionally, presence chemical contaminants such heavy metals (e.g., mercury lead), microplastics, per- polyfluoroalkyl substances (PFASs) increasing concern due their potential accumulate food chain adversely affect health. biogenic amines formed during degradation proteins allergens present certain species also contribute challenges. This review aims address nutritional value health-promoting effects while exploring multifaceted risks associated with contamination, pollutants, naturally occurring substances. Emphasis placed on enhanced surveillance, traceability, sustainable aquaculture practices, regulatory harmonization effective strategies controlling thereby contributing safer supply chain.
Language: Английский
Citations
0
International Journal of Food Microbiology, Journal Year: 2023, Volume and Issue: 407, P. 110422 - 110422
Published: Sept. 30, 2023
This work is the first of its kind to report a whole-year and coastal-wide surveillance antimicrobial resistance (AMR) Escherichia coli with samples from EU imposed Norwegian programme for marine bivalves. In total, 390 bivalve collected January December in 2016 at 59 different harvest locations, were examined. The occurrence resistant E. relation concentration was also analysed. From each sample (n = 261), one isolate susceptibility tested against panel 14 antimicrobials ten classes. least 8.4 %. Resistance tetracycline most commonly detected (5.7 %), followed by ampicillin (4.6 %) sulfamethoxazole (3.1 %). extended spectrum cephalosporin (ESC)-resistant coli, quinolone-resistant (QREC) carbapenem-resistant Enterobacteriaceae (CRE) through selective screening 3.3 %, 12.8 % none samples, respectively. Among ESC-resistant blaCTX-M-15 gene nine isolates, where two isolates carried blaCMY-42 gene, blaCTX-M-3 blaCTX-M-1 one. One ESC due n.-42C>T mutation AmpC gene. Only presence QREC clustered significantly (p < 0.013) space including locations. An increased risk (OR 9.4) detecting or found concentrations above threshold Class A direct distribution market (i.e. 230 coli/100 g). However, five 26 positive had levels below threshold, thus areas cleared sale. 17 subjected whole genome sequencing, originated great scallops flat oysters, which are often consumed raw lightly processed. these belonged high-risk clone sequence type 131 plasmid born senB encoding Shigella enterotoxin 2 (ShET2) attributed cause watery diarrhoea infections caused Enteroinvasive (EIEC). Thus, our study shows that there potential transmission pathogenic consumers products.
Language: Английский
Citations
5
Microbial Genomics, Journal Year: 2023, Volume and Issue: 9(12)
Published: Dec. 12, 2023
Enterococci, especially Enterococcus faecium , are one of today’s leading causes multidrug-resistant infections in hospital settings. The marine environment may harbour enterococci, but its role as an evolutionary niche and a vector for the spread enterococci is sparsely investigated. Hence, by applying bivalves sentinel tool, this study aimed to describe prevalence enterocooci along Norwegian coast addition phylogeny E. particular. Enterococci batch samples bivalves, harvested from 86 different locations, were quantitatively examined culture-dependent most probable number (MPN) method. Isolates identified MALDI-TOF-MS prior antimicrobial susceptibility testing broth microdilution. In-detail analyses representative selection isolates ( n =148) done Illumina whole-genome sequencing, assembled genomes compared closed public databases commensal clinical Norway. Diversity among within same sample was also explored. detected 287 471 bivalve samples, low concentrations with median value <18 MPN /100 g. From positive 479 belonging ten species, where =247), hirae =114) faecalis =66) frequently found. Resistance towards or more agents observed 197 (41 %), none showed acquired resistance vancomycin linezolid. Phylogenetic revealed high diversity that dominated strains non-clinical setting clade A2 =85) B lactis ) =60). Only three belonged hospital-associated A1 (ST80 ST117). Two these clustered isolate hospitalized patient non-hospitalized person. This demonstrated prevalence, levels resistance. population very few have arisen human healthcare system. A systematic surveillance target micro-organisms methods examining multiple provides important data assess enterococcal phylogeny, level faecal pollution environment.
Language: Английский
Citations
2
Naturen, Journal Year: 2024, Volume and Issue: 148(2), P. 76 - 84
Published: April 30, 2024
Citations
0
Food Control, Journal Year: 2024, Volume and Issue: unknown, P. 111127 - 111127
Published: Dec. 1, 2024
Language: Английский
Citations
0