Microorganisms
isolated
from
restricted
environments
may
have
a
differentiated
secondary
metabolic
potential,
producing
substances
in
the
culture
medium
that
be
bioactive
and
of
pharmacological
interest.The
objective
this
project
was
to
investigate
metabolite
palitantin,
originally
media
fungus
Penicillium
palitans.The
fungal
strain
Geomyces
sp.AMF1,
Antarctica,
produces
(-)-palitantine
medium.The
realization
fractional
experimental
design
allowed
establishing
growth
conditions
improved
palitantin
production,
making
it
possible
obtain
significant
amounts
for
its
derivatization.Two
stereoisomers
palitantol
were
obtained
by
reduction
with
sodium
borohydride
triacetoxyborohydride.Reduction
products
separated
HPLC
identified
spectroscopic
analysis.Palitantin
two
sent
evaluation
bioassays
cytotoxic,
antiplamodial,
antileshimania
antimicrobial
activity,
but
no
biological
activity
observed.The
second
part
research
aimed
at
isolating
identifying
metabolites
produced
Peroneutypa
sp,
M16,
sea
cucumber.The
defatted
organic
fraction
subjected
series
chromatographic
separations.Fractions
selected
investigated
M16As44,
M16As51
M16As54,
which
submitted
separations
purification
HPLC-UV,
leading
isolation
38-44.These
completely
analyzing
their
data,
40
43
are
unpublished
literature.
Information
generated
from
longitudinally-sampled
microbial
data
has
the
potential
to
illuminate
important
aspects
of
development
and
progression
for
many
human
conditions
diseases.
Identifying
biomarkers
their
time-varying
effects
can
not
only
advance
our
understanding
pathogenetic
mechanisms,
but
also
facilitate
early
diagnosis
guide
optimal
timing
interventions.
However,
longitudinal
predictive
modeling
highly
noisy
dynamic
(e.g.,
metagenomics)
poses
analytical
challenges.
To
overcome
these
challenges,
we
introduce
a
robust
interpretable
machine-learning-based
microbiome
analysis
framework,
LP-Micro,
that
encompasses:
(i)
feature
screening
via
polynomial
group
lasso,
(ii)
disease
outcome
prediction
implemented
machine
learning
methods
XGBoost,
deep
neural
networks),
(iii)
association
testing
between
time
points,
features,
outcomes
permutation
importance.
We
demonstrate
in
simulations
LP-Micro
identify
incident
disease-related
taxa
offers
improved
accuracy
compared
existing
approaches.
Applications
two
studies
with
clinical
childhood
dental
weight
loss
following
bariatric
surgery
yield
consistently
high
accuracy.
The
identified
critical
points
are
informative
aligned
expectations.
Animals,
Journal Year:
2023,
Volume and Issue:
13(19), P. 3058 - 3058
Published: Sept. 29, 2023
The
Chinese
mitten
crab
(Eriocheir
sinensis)
has
significant
economic
potential
in
both
the
domestic
and
global
markets.
hemolymph
microbiota
is
known
to
play
a
critical
role
regulating
physiological
biochemical
functions
crustaceans.
However,
study
of
E.
sinensis
response
infections
not
been
undertaken.
In
this
study,
changes
composition
function
infected
with
either
Staphylococcus
aureus
(Sa)
or
Aeromonas
hydrophila
(Ah)
were
investigated
using
16S
rRNA
sequencing,
phosphate
buffer
saline
(PBS)
injection
serving
as
control.
Results
showed
that
dominant
Proteobacteria,
Bacteroidota,
Firmicutes.
relative
abundance
phyla
Firmicutes,
Bdellovibrionota,
Myxococcota
was
significantly
reduced
Sa
Ah
groups
compared
PBS
group.
At
genus
level,
group,
increase
Flavobacterium
found
groups.
analysis
functional
profile
pathways
related
‘cell
growth
death’,
‘metabolism
terpenoids
polyketides’,
‘cancers’,
‘lipid
metabolism’,
‘neurodegenerative
diseases’,
other
amino
acids’,
‘xenobiotics
biodegradation
‘circulatory
system
endocrine
system’
predominant
Meanwhile,
genetic
information
progressing’,
such
‘translation’,
‘metabolic
‘cellular
processes
signaling’,
enriched
This
revealed
effects
pathogens
(S.
A.
hydrophila)
on
maintenance
sinensis.
It
shed
light
mechanisms
employed
by
under
pathogen
stimulation.
Environmental Microbiology Reports,
Journal Year:
2024,
Volume and Issue:
16(1)
Published: Feb. 1, 2024
Abstract
Ascidians
are
marine
invertebrates
known
to
occasionally
host
symbiotic
crustaceans.
Although
the
microbiomes
of
both
ascidians
and
free‐living
crustaceans
have
been
characterized,
there
is
no
documentation
microbial
communities
in
an
ascidian–crustacean
symbiosis.
Samples
solitary
ascidian
Ascidia
sydneiensis
ambient
seawater
were
collected
Belize.
Four
amphipod
retrieved
from
branchial
sac
animal,
their
compared
with
those
(tunic
compartments)
seawater.
Microbiome
richness
diversity
differed
significantly
between
sample
types,
exhibiting
lower
than
tunic
samples.
composition
also
types
among
all
pairwise
comparisons,
except
for
microbiomes.
Differential
operational
taxonomic
unit
(OTU)
analyses
revealed
that
only
3
out
2553
OTUs
had
different
relative
abundances
amphipods
sacs,
whereas
72
315
Thus,
body
compartments
A
.
hosted
distinct
microbiomes,
contained
resembling
region
they
inhabit
(i.e.,
sac),
suggesting
environmental
filtering
co‐evolutionary
processes
determinants
microbiome
within
symbioses.
PeerJ,
Journal Year:
2024,
Volume and Issue:
12, P. e18298 - e18298
Published: Nov. 28, 2024
Background
Understanding
the
dynamics
of
gut
microbiota
in
sea
urchins
is
crucial
for
comprehending
ecological
balance
marine
ecosystems.
The
plays
a
vital
role
nutrient
metabolism,
immune
system
modulation,
and
pathogen
protection.
microbial
composition
naturally
occurring
urchin
Tripneustes
ventricosus
have
yet
to
be
thoroughly
investigated.
We
hypothesized
T.
Caribbean,
varies
across
life
stages
seasons.
Methods
Thirty-six
large
individuals
six
small
(42
animals)
were
collected
from
shallow
waters
on
northeastern
coast
Puerto
Rico
February
August
2019.
fecal
pellet’s
was
characterized
by
sequencing
V4
region
16S
rRNA
gene.
Results
found
significant
differences
pellet
between
seasons
stages.
Phylum
Bacteroidota
had
greater
relative
abundance
August,
while
Firmicutes
more
dominant
February.
Propionigenium
Roseimarinus
Candidatus
Hepatoplasma,
Kistimonas
Differences
digest
not
urchins,
but
displayed
slightly
higher
diversity
dominance
Proteobacteria,
exhibited
Fusobacteria
Desulfobacterota.
However,
genera
Ferrominas
counts
significantly
lower
individuals.
Discussion
This
first
report
this
species
Caribbean
adds
our
comprehension
white
collection
periods
size
classes,
highlighting
dynamic
nature
microbiota.
