Characterization of novel cold-active chitin deacetylase for green production of bioactive chitosan

AMB Express, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 4, 2025

Abstract A Novel cold-active chitin deacetylase from Shewanella psychrophila WP2 (SpsCDA) was overexpressed in Escherichia coli BL21 and employed for deacetylation of to chitosan. The produced chitosan characterized, its antifungal activity investigated against Fusarium oxysporum . purified recombinant SpsCDA appeared as a single band on SDS-PAGE at approximately 60 kDa, specific 92 U/mg. optimum temperature pH were 15 °C 8.0, respectively, the enzyme significantly enhanced presence NaCl. bioconversion by accomplished 72 h, yield 69.2%. solubility estimated be 73.4%, degree 78.1%. molecular weight 224.7 ± 8.4 kDa with crystallinity index (CrI) value 18.75. Moreover, FTIR XRD spectra revealed characteristic peaks enzymatically compared standard chitosan, indicating their structural similarity. inhibited spore germination F. minimum inhibitory concentration (MIC) 1.56 mg/mL. potential effect is attributed inhibition accompanied ultrastructural damage membranes leakage cellular components, evidenced transmission electron microscopy (TEM), accumulation reactive oxygen species (ROS) that confirmed fluorescence microscopy. This study shed light S. provides candidate green preparation

Language: Английский

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Biogenic Silver Nanoparticles Produced by Soil Rare Actinomycetes and Their Significant Effect on Aspergillus-derived mycotoxins

Microorganisms, Journal Year: 2023, Volume and Issue: 11(4), P. 1006 - 1006

Published: April 12, 2023

The current investigation addressed the green synthesis of silver nanoparticles (AgNPs) using newly isolated silver-resistant rare actinomycetes, Glutamicibacter nicotianae SNPRA1 and Leucobacter aridicollis SNPRA2, investigated their impact on mycotoxigenic fungi Aspergillus flavus ATCC 11498 ochraceus 60532. formation AgNPs was evidenced by reaction's color change to brownish appearance characteristic surface plasmon resonance. transmission electron microscopy biogenic produced G. L. SNPRA2 (designated Gn-AgNPs La-AgNPs, respectively) revealed generation monodispersed spherical with average sizes 8.48 ± 1.72 nm 9.67 2.64 nm, respectively. Furthermore, XRD patterns reflected crystallinity FTIR spectra demonstrated presence proteins as capping agents. Both bioinspired exhibited a remarkable inhibitory effect conidial germination fungi. caused an increase in DNA protein leakage, suggesting disruption membrane permeability integrity. Interestingly, completely inhibited production total aflatoxins ochratoxin A at concentrations less than 8 μg/mL. At same time, cytotoxicity investigations low toxicity against human skin fibroblast (HSF) cell line. feasible biocompatibility HSF cells up 10 μg/mL IC50 values were 31.78 25.83 for present work sheds light antifungal prospect actinomycetes promising candidates combat mycotoxin food chains nontoxic doses.

Language: Английский

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Tellurium and Nano-Tellurium: Medicine or Poison?

Nanomaterials, Journal Year: 2024, Volume and Issue: 14(8), P. 670 - 670

Published: April 12, 2024

Tellurium (Te) is the heaviest stable chalcogen and a rare element in Earth’s crust (one to five ppb). It was discovered gold ore from mines Kleinschlatten near present-day city of Zlatna, Romania. Industrial other applications Te focus on its inorganic forms. can be toxic animals humans at low doses. Chronic tellurium poisoning endangers kidney, liver, nervous system. However, effective against bacteria able destroy cancer cells. also used develop redox modulators enzyme inhibitors. Soluble salts that contain had role as therapeutic antimicrobial agents before advent antibiotics. The pharmaceutical use not widespread due narrow margin between beneficial doses, but there are differences measure toxicity based form. Nano-tellurium (Te-NPs) has several applications: it act an adsorptive agent remove pollutants, antibacterial coating, photo-catalysis for degradation dyes, conductive electronic materials. Nano-sized particles most promising produced both chemical biological ways. Safety assessments essential determine potential risks benefits using compounds various applications. Future challenges directions developing nano-materials, nano-alloys, nano-structures still open debate.

Language: Английский

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Production and Biological Characterization of Nanoparticles from Mangrove-Associated Microorganisms

Published: Jan. 1, 2025

Language: Английский

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Metal Nanomaterials and Hydrolytic Enzyme-Based Formulations for Improved Antifungal Activity

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(14), P. 11359 - 11359

Published: July 12, 2023

Active research of metal-containing compounds and enzymes as effective antifungal agents is currently being conducted due to the growing resistance problem. Metals are attracting special attention wide variety ligands that can be used for them, including chemically synthesized naturally obtained variants a result so-called "green synthesis". The main mechanism action metals triggering generation accumulation reactive oxygen species (ROS). Further ROS on various biomolecules nonspecific. Various hydrolytic (glucanases proteases), in turn, exhibit properties by affecting structural elements fungal cells (cell walls, membranes), quorum sensing molecules, own protective (mycotoxins antibiotics), proteins responsible adhesion formation stable, highly concentrated populations form biofilms. A substrate range allows use mechanisms their actions. In this review, we discuss prospects combining two different types (metals enzymes) against mycelial fungi yeast cells. Special paid possible influence activity effects compounds.

Language: Английский

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Mangrove synthesized bio-nanomaterial and its applications: A review

Environmental Nanotechnology Monitoring & Management, Journal Year: 2023, Volume and Issue: 20, P. 100866 - 100866

Published: Aug. 16, 2023

Mangroves are a transitional ecosystem between terrestrial and marine environments, hosting rich diversity of flora fauna that adapt to both environments considered biodiversity hotspots. These groups salt-tolerant found on protected shorelines. minimize environmental disasters, including cyclones tsunamis, from damaging the coastline. Between shorelines intertidal zone, mangroves act as CO2 sinks. threatened by various anthropogenic pressures pollution due industrial effluents discharged near coastal ecosystems, affecting microorganisms' taxonomic functional mangrove stability. Mangrove microorganisms plant enzymatic activities have potential for biotechnological applications. resources promising synthesizing bio-nanomaterials using bioactive compounds can be helpful biomedical Bio-nanomaterials synthesized highly efficient in applications decontamination. several biomolecules derived plants precursors nanoparticle synthesis. Due high surface area, biocompatibility, low toxicity, advantages sustainable utilization mangroves. The present review paper highlights application natural resource towards greener approach

Language: Английский

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Novel Biosynthesis and Evaluation Cytotoxic Effect of Tellurium Nano-Particle on Breast Cancer Using Co-Culture of Spirulina and Lentinula Edodes

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: June 18, 2024

Introduction Bio-based nanoparticle production has been an alternative method for preparing nanoparticles pharmaceutical and biomedical applications. This study aims to evaluate the of tellurium (TeNPs) using co-culture algae fungi finally investigate cytotoxicity on cancer cell lines MDA-MB-468 healthy line HFF. Methods The TeNPs was done fungi. Using Tyndall effect, FTIR, FESEM, were evaluated. Finally, MTT assay, toxicity produced HFF investigated. Results Data showed that it is possible produce co-cultivation Also, had a size between 20 30 nm. Examining effects created this inhibitory effect cells, while does not affect cells. Conclusion In study, biosynthesis performed first time. data use can inhibit growth human breast

Language: Английский

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Carbon Nanodot–Microbe–Plant Nexus in Agroecosystem and Antimicrobial Applications

Nanomaterials, Journal Year: 2024, Volume and Issue: 14(15), P. 1249 - 1249

Published: July 25, 2024

The intensive applications of nanomaterials in the agroecosystem led to creation several environmental problems. More efforts are needed discover new insights nanomaterial-microbe-plant nexus. This relationship has dimensions, which may include transport different plant organs, nanotoxicity soil microbes and plants, possible regulations. review focuses on challenges prospects nexus under conditions. previous nano-forms were selected this study because rare, published articles such nanomaterials. Under study's nexus, more carbon nanodot-microbe-plant discussed along with role frontier nano-tellurium-microbe Transport organs applications, translocation these nanoparticles besides their expected will be also reported current study. Nanotoxicity plants was investigated by taking account morpho-physiological, molecular, biochemical concerns. highlights regulations a focus risk at ecological level risks human health, scientific organizational levels. opens many windows studies near future.

Language: Английский

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Bacterial Nanofactories: An In-Depth Insight to Potential Green Technology

International Journal of Precision Engineering and Manufacturing-Green Technology, Journal Year: 2024, Volume and Issue: 12(2), P. 691 - 717

Published: Dec. 6, 2024

Language: Английский

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Characterization of a Metallic-Ions-Independent L-Arabinose Isomerase from Endophytic Bacillus amyloliquefaciens for Production of D-Tagatose as a Functional Sweetener

Fermentation, Journal Year: 2023, Volume and Issue: 9(8), P. 749 - 749

Published: Aug. 12, 2023

D-Tagatose is a low-calorie sugar substitute that has gained increased attention as functional sweetener owing to its nutraceutical and prebiotic properties. Traditionally, D-tagatose produced via the enzymatic conversion of L-galactose by L-arabinose isomerase (L-AI). Nonetheless, most reported L-AI enzymes are ion-dependent requiring Mn2+ and/or Co2+ cofactors for their reactions, which limits application due safety health concerns. Herein, we addressed facile bioconversion using novel recombinant metallic-ions-independent derived from endophytic Bacillus amyloliquefaciens CAAI isolated cantaloupe fruits. The ORF (1500 bp) gene (araA) was cloned over-expressed in Escherichia coli. enzyme (BAAI) purified homogeneity Ni-NTA affinity chromatography, yielding single distinct band with an apparent molecular mass approximately 59 kDa deduced SDS-PAGE analysis. showed optimum activity at pH temperature 7.5 45 °C, respectively, obvious presence ethylenediaminetetraacetic acid (EDTA), indicating metallic-ions independence BAAI. Km values BAAI D-galactose were 251.6 mM 92.8 mM, respectively. catalytic efficiency (kcat/Km) found be 2.34 46.85 mM–1 min–1, results revealed production 47.2 g/L (100 g/L) 47.2% metallic-ions-free reaction system could implemented safe-production food-grade sweetener, D-tagatose.

Language: Английский

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