Roles and mechanisms of gapA and gap -encoded proteins in cronobacter sakazakii adhesion to and invasion of intestinal cells and neonatal rats DOI Creative Commons
Chumin Zhao, Ping Li,

Dongdong Zhu

et al.

Virulence, Journal Year: 2024, Volume and Issue: 16(1)

Published: Dec. 30, 2024

Cronobacter sakazakii (C. sakazakii) is a foodborne pathogen whose initial process involves intestinal cell adhesion mediated by numerous virulence factors encoded in various genes. The key metabolic enzyme, glyceraldehyde-3-phosphate dehydrogenase (GAPDH, also known as GapA), gapA the glycolysis pathway and acts an adhesin some bacteria. In C. sakazakii, there enzyme gluconeogenesis pathway, Gap, with type I GAPDH erythrose-4-phosphate activities gap. This study aimed to investigate properties of GapA Gap invasion HCT-8 HIEC-6 cells damage colon brain neonatal rats, gene silencing. addition, role both recombinant proteins was investigated, their inducting inflammatory cytokine expression assessed ELISA Western blot. Silencing or gap decreased viability swimming motility bacterial reduced types cells. Both contributed cells, enhanced protein phosphorylation NF-κB, induced expression. Finally, silenced weakened rats. conclusion, we demonstrated for first time rats induction responses.

Language: Английский

Probiotics fortify intestinal barrier function: a systematic review and meta-analysis of randomized trials DOI Creative Commons

Yanfei Zheng,

Zengliang Zhang,

Ping Tang

et al.

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: April 24, 2023

Background Probiotics play a vital role in treating immune and inflammatory diseases by improving intestinal barrier function; however, comprehensive evaluation is missing. The present study aimed to explore the impact of probiotics on related function, inflammation, microbiota composition. A systematic review meta-analyses were conducted. Methods Four major databases (PubMed, Science Citation Index Expanded, CENTRAL, Embase) thoroughly searched. Weighted mean differences calculated for continuous outcomes with corresponding 95% confidence intervals (CIs), heterogeneity among studies was evaluated utilizing I2 statistic (Chi-Square test), data pooled using random effects meta-analyses. Results Meta-analysis from total 26 RCTs (n = 1891) indicated that significantly improved gut function measured levels TER (MD, 5.27, CI, 3.82 6.72, P < 0.00001), serum zonulin (SMD, -1.58, -2.49 -0.66, 0.0007), endotoxin -3.20, -5.41 -0.98, 0.005), LPS -0.47, -0.85 -0.09, 0.02). Furthermore, probiotic groups demonstrated better efficacy over control reducing factors, including CRP, TNF-α, IL-6. can also modulate structure boosting enrichment Bifidobacterium Lactobacillus. Conclusion work revealed could improve alleviate inflammation microbial dysbiosis. Further high-quality are warranted achieve more definitive conclusion. Clinical trial registration https://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=281822 , identifier CRD42021281822.

Language: Английский

Citations

71
Caco2/HT-29 In Vitro Cell Co-Culture: Barrier Integrity, Permeability, and Tight Junctions’ Composition During Progressive Passages of Parental Cells DOI Creative Commons
Elena Donetti, Paola Bendinelli, Margherita Correnti

et al.

Biology, Journal Year: 2025, Volume and Issue: 14(3), P. 267 - 267

Published: March 6, 2025

Epithelial linings are crucial for the maintenance of physiological barriers. The intestinal epithelial barrier (IEB) consists enterocytes through tight junctions and mucus-secreting cells can undergo modifications throughout life. To reproduce as closely possible IEB main features over time, in vitro co-cultures Caco2/HT-29 70/30 formed by parental Caco2 HT-29 sub-cultivated more than 40 passages were set up. measurements transepithelial electrical resistance (TEER) identified two populations: TEER (PC) with values > 50 Ωcm2 fewer passages, leaky (LC) < passages. In LC, paracellular permeability increased parallel. By immunofluorescence Western blot analysis, an increase claudin 2 was observed LC vs. PC, no differences occludin expression. MUC-2 immunoreactivity stronger PC LC. also showed enhanced vulnerability to TNFα+IFN-γ. These results morpho-functional reported human leaky/aged gut support usefulness our cell model studying molecular processes underlying these testing drug/nutraceutical treatments ameliorate aging.

Language: Английский

Citations

1
Natural flavones from edible and medicinal plants exhibit enormous potential to treat ulcerative colitis DOI Creative Commons
Qiang Lü,

Yuhong Xie,

Jingbin Luo

et al.

Frontiers in Pharmacology, Journal Year: 2023, Volume and Issue: 14

Published: June 1, 2023

Ulcerative colitis (UC) is a chronic aspecific gut inflammatory disorder that primarily involves the recta and colons. It mostly presents as long course of repeated attacks. This disease, characterized by intermittent diarrhoea, fecal blood, stomachache, tenesmus, severely decreases living quality sick persons. UC difficult to heal, has high recurrence rate, tightly related incidence colon cancer. Although there are number drugs available for suppression colitis, conventional therapy possesses certain limitations severe adverse reactions. Thus, it extremely required safe effective medicines naturally derived flavones exhibited huge prospects. study focused on advancement from edible pharmaceutical plants treating colitis. The underlying mechanisms natural-derived in were closely linked regulation enteric barrier function, immune-inflammatory responses, oxidative stress, microflora, SCFAs production. prominent effects safety make them promising candidate treatment.

Language: Английский

Citations

15
The Kynurenine Pathway in Gut Permeability and Inflammation DOI Creative Commons
Rowan Kearns

Inflammation, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 10, 2024

Language: Английский

Citations

5
Evaluating the health risk of probiotic supplements from the perspective of antimicrobial resistance DOI Creative Commons

Qiwen Tian,

Hailv Ye,

Xuan Zhou

et al.

Microbiology Spectrum, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 10, 2024

ABSTRACT Antimicrobial resistance remains a public health threat. Probiotics harboring antimicrobial resistant genes (ARGs) have, in recent years, been considered potential risk. Studies conducted on probiotics from increasingly popular supplements have raised the possibility of transmitting ARGs to commensals human gut, concomitantly establishing reservoir and risking acquisition by opportunistic pathogens. Building our previous study that reported multiple antibiotic supplements, this research, we attempted detect their may account for phenotypes. responsible tetracycline, macrolide, aminoglycoside, glycopeptide were prevalent probiotics. Through laboratory adaptive evolution studies, also show streptomycin-adapted gained erythromycin, doxycycline more effectively than non-adapted ones. When co-incubated with Enterococcus faecalis , Escherichia coli or Staphylococcus aureus Caco-2 and/or HCT-116 cells, streptomycin was transferred adapted generate transconjugants at frequencies comparable higher other studies through filter mating. Consistently, conferring ( aadA ) erythromycin [ erm(B)−1 ] detected E. S. transconjugants, respectively, after co-incubation cells. both transconjugant same Our data future comparative genomics metagenomics animal models healthy, immunocompromised, antibiotic-treated cohorts will contribute comprehensive understanding probiotic consumption, application, safety. IMPORTANCE are becoming popular, promising applications food medicine, but risk transferring disease-causing bacteria has concerns. drugs. Streptomycin-adapted antibiotics Importantly, showed could be intestinal resistance, which initially absent recipient bacteria, transconjugants. build foundation humans leveraging advanced approaches clarify long-term consumption.

Language: Английский

Citations

5
Oxyberberine alleviates lipopolysaccharide-induced intestinal barrier disruption and inflammation in human colonic Caco-2 cells in vitro DOI Creative Commons
Cailan Li, Jiahao Wang, Hongmei Yang

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 7, 2025

Oxyberberine (OBB) is a naturally occurring isoquinoline alkaloid that believed to possess various health-promoting properties, including anti-fungus, hepatoprotection, anti-inflammation, and anti-intestinal mucositis effects. Despite several studies reporting the health benefits of OBB in treating ulcerative colitis (UC), its specific mechanism action has yet be fully elucidated. This investigation designed explore potential protective efficacy latent using an vitro model UC-like inflammatory intestinal cells. Caco-2 cells were pretreated with subsequently exposed lipopolysaccharide (LPS). The transepithelial electrical resistance (TEER), paracellular permeability, distribution expression tight- adherent junction proteins determined assess barrier integrity. levels proinflammatory cytokines, reactive oxygen species (ROS), Nrf2, NF-κB signaling cascade analyzed via ELISA, qRT-PCR, immunofluorescence, or Western blotting. was found mitigate effects LPS on cell monolayers, as evidenced by improvement TEER decrease FITC-dextran flux. Moreover, ameliorated LPS-induced tight markers, ZO-1, occludin, E-cadherin. In addition, treatment effectively inhibited increases ROS, apoptosis, Keap1 decreases Nrf2 HO-1. elevations nuclear p65 p-IκBα suppressed OBB. ML385, antagonist abolished role pronounced beneficial effect damage enteral function, regulation Nrf2/NF-κB pathway important responsible for protection afforded

Language: Английский

Citations

0
Immunomodulatory Properties of Live and Thermally‐Inactivated Food‐Origin Lactic Acid Bacteria—In Vitro Studies DOI
Wioletta Mosiej, Ewa Długosz, Marcin Kruk

et al.

Molecular Nutrition & Food Research, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

ABSTRACT The study investigates the strain‐specific immunomodulatory properties of live and thermally‐inactivated (TI) lactic acid bacteria (LAB) derived from traditional Polish fermented foods, focusing on their potential as probiotics postbiotics. LAB strains, known for role in food fermentation, were assessed ability to influence cytokine production THP‐1 macrophages, maintain intestinal epithelial barrier integrity Caco‐2 monolayers, exhibit antioxidant activity, produce specific organic acids sugars. research demonstrated that strains significantly upregulated anti‐inflammatory IL‐10, particularly under inflammatory conditions, while TI exhibited notable properties. showed a greater protect function reduce pro‐inflammatory secretion than suggesting promising findings underscore demonstrating postbiotic derivatives can differently inflammation compared bacteria, highlighting immune‐enhancing agents, capable modulating immune responses offering therapeutic benefits against inflammation‐related disorders. However, limitations vitro models highlight need further vivo clinical studies validate these effects fully uncover health humans.

Language: Английский

Citations

0
The Potential Role of Probiotic and Postbiotic Mixture in Ameliorating Oxidative Stress, Effect on Kidney and Intestine of Male Wistar Rats Exposed to Copper DOI
Zahra Keshtmand,

Sepideh Sayadi,

Hossein Kashfi

et al.

Journal of Pharmaceutical Innovation, Journal Year: 2025, Volume and Issue: 20(2)

Published: April 1, 2025

Language: Английский

Citations

0
Small Intestine on a Chip Demonstrates Physiologic Mucus Secretion in the Presence of Lacticaseibacillus rhamnosus Biofilm DOI Creative Commons
Sanat Kumar Dash, Cláudia N. H. Marques, Gretchen J. Mahler

et al.

Biotechnology and Bioengineering, Journal Year: 2025, Volume and Issue: unknown

Published: April 8, 2025

ABSTRACT The small intestine is an area of the digestive system difficult to access using current medical procedures, which prevents studies on interactions between food, drugs, intestinal epithelium, and resident microbiota. Therefore, there a need develop novel microfluidic models that mimic biological mechanical environments. These can be used for drug discovery disease modeling have potential reduce reliance animal models. goal this study was chip with both enterocyte (Caco‐2) goblet (HT29‐MTX) cells cocultured Lacticaseibacillus rhamnosus biofilms, one several genera present in L. introduced following establishment epithelial barrier. shear stress within device kept lower physiological range (0.3 mPa) enable biofilm development over vitro epithelium. barrier differentiated after 5 days dynamic culture cell polarity permeability similar human intestine. presence biofilms did not alter barrier's conditions. Under fluid flow, complete model remained viable functional more than days, while static only 1 day. increased secretion acidic neutral mucins by Furthermore, also showed MUC2 production, dominant gel‐forming mucin This builds previous publications as it establishes stable environment closely mimics vivo conditions physiology, food‐intestinal interactions, development.

Language: Английский

Citations

0
Postbiotic Potential of Newly Isolated Riboflavin-Overproducing Lactiplantibacillus plantarum Strains DOI Creative Commons
Angela Scauro, Maria Teresa Rocchetti, Mario Soccio

et al.

Probiotics and Antimicrobial Proteins, Journal Year: 2025, Volume and Issue: unknown

Published: April 23, 2025

Language: Английский

Citations

0