The Potential Role of Probiotic and Postbiotic Mixture in Ameliorating Oxidative Stress, Effect on Kidney and Intestine of Male Wistar Rats Exposed to Copper

Journal of Pharmaceutical Innovation, Journal Year: 2025, Volume and Issue: 20(2)

Published: April 1, 2025

Language: Английский

---

Immune-Stimulating Potential of Lacticaseibacillus rhamnosus LM1019 in RAW 264.7 Cells and Immunosuppressed Mice Induced by Cyclophosphamide

Microorganisms, Journal Year: 2023, Volume and Issue: 11(9), P. 2312 - 2312

Published: Sept. 13, 2023

Probiotics, including Lacticaseibacillus rhamnosus (L. rhamnosus), have gained recognition for their potential health benefits, such as enhancing immune function, maintaining gut health, and improving nutrient absorption. This study investigated the effectiveness of L. LM1019 (LM1019) in function. In RAW 264.7 cells, demonstrated dose-dependent stimulation by increasing nitric oxide production, gene expression proinflammatory cytokines, inducible synthase (iNOS) cyclooxygenase-2 (COX-2). These effects were mediated through activation mitogen-activated protein kinases (MAPKs) nuclear factor-kappa B (NF-κB) translocation without inducing cytotoxicity. Furthermore, orally administered was evaluated immunosuppressed mice induced cyclophosphamide (CTX). High-dose administration significantly increased subpopulations lymphocytes, specifically helper T cells (CD4+), well two subtypes natural killer (NK) namely, IFN-γ+ granzyme B+ NK cells. Additionally, at a high dose led to elevated levels IFN-γ IL-12, compared CTX-treated mice. findings highlight system. The contributes growing body research on beneficial probiotics

Language: Английский

---

Short communication: A novel multispecies bacteria-based direct-fed microbial supports in vitro gut barrier integrity challenged with a pathogen or pro-inflammatory cytokines

Journal of Animal Science, Journal Year: 2024, Volume and Issue: 102

Published: Jan. 1, 2024

Abstract We conducted two experiments to evaluate the effects of a novel bacterial-based direct-fed microbial (DFM) on intestinal barrier integrity using in vitro transepithelial electrical resistance (TEER) assay. In experiment 1, human-derived Caco-2 cells received or not (CON) DFM containing Ligilactobacillus (formerly Lactobacillus) animalis 506, Propionibacterium freudenreichii 507, Bacillus paralicheniformis 809, and B. subtilis 597 (BDP; BOVAMINE DEFEND® Plus) at rate 1 × 108 CFU/transwell. Concurrently with treatment application (CON BDP), pathogenic challenge Clostridium perfringens type A was added alone (PAT) BDP (PAT + BDP) 2.8 107 CFU/transwell 2 factorial arrangement. 2, were also assigned design CON then, h post-treatment administration mixture tumor necrosis factor-alpha (TNF-α) interferon-gamma (IFN-γ) (CYT) (CYT 10:1 ratio, respectively. both experiments, TEER measured for 18 h. pathogen hour interaction observed (P < 0.0001). Adding PAT initially tended increase vs. from 1.1 2.2 ≤ 0.09), increased 3.2 0.01), but reduced 5.4 end experimental period 18.4 0.01). On other hand, adding DFM, without challenge, yielded greater CON-CON CON-PAT most 0.04). similar detected reported The CYT mean compared all treatments remainder study 0.03). BDP-CYT able maintain epithelial when throughout 0.03), exception being = 0.20). Moreover, BDP-CON 0.04) h, comparison 4.3 post-DFM into cells. summary, C. pro-inflammatory cytokine cocktail compromised cell monolayers vitro, whereas multispecies bacteria-based counteracted these damaging effects.

Language: Английский

---

Streptococcus salivarius ameliorates the destructive effect on the epithelial barrier by inhibiting the growth of Prevotella melaninogenica via metabolic acid production

Molecular Oral Microbiology, Journal Year: 2024, Volume and Issue: 39(5), P. 407 - 416

Published: April 30, 2024

Oral lichen planus (OLP) is one of the most common oral mucosal diseases, exhibiting a higher prevalence in women than men, but its pathogenesis still unclear. Current research suggests that microbial dysbiosis may play an important role OLP. Our previous has found increase Prevotella melaninogenica and decrease Streptococcus salivarius have been identified as potential pathogenic factor Consequently, objective this study to examine whether S. can counteract detrimental effects P. on integrity epithelial barrier function.

Language: Английский

---

The Role of Microbiome and Probiotics in Chemo‐Radiotherapy‐Induced Diarrhea: A Narrative Review of the Current Evidence

Cancer Reports, Journal Year: 2024, Volume and Issue: 7(10)

Published: Oct. 1, 2024

ABSTRACT Background In this article, we review the most recent research on probiotics effects diarrhea in both human and animal models of condition along with therapeutic potential these compounds based their findings. Recent Findings Nearly 50%–80% cancer patients experience chemotherapy‐induced (CID), serious gastrointestinal toxicity chemotherapeutic radiation regimens that leads to prolonged hospitalizations, cardiovascular problems, electrolyte imbalances, disruptions treatment, poor prognosis, death. CID is typically categorized as osmotic diarrhea. The depletion colonic crypts villi by radiotherapy chemotherapy agents interferes absorptive function intestine, thereby decreasing absorption chloride releasing water into intestinal lumen. Probiotic supplements have been found be able reverse damage caused chemo‐radiation therapy promoting growth crypt reducing inflammatory pathways. addition, they support modulation immunological angiogenesis responses gut well metabolism certain digestive enzymes altering microbiota. Conclusion Beyond benefits probiotics, additional clinical required clarify effective strain combinations dosages for preventing

Language: Английский

---

A probiotic multi-strain mixture combined with hydroxyectoine improves intestinal barrier function by alleviating inflammation in lipopolysaccharide stimulated differentiated Caco-2 cells

Food & Function, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Freeze-dried formula containing three probiotic strains and hydroxyectoine reduces LPS-induced inflammation in Caco-2 cells, assessed via RT-PCR, western blot, ELISA, immunofluorescence for tight junction integrity.

Language: Английский

---

Evaluation of the Effect of Probiotic Supplementation on Intestinal Barrier Integrity and Epithelial Damage in Colitis Disease: A Systematic Review

Nutrition Reviews, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 26, 2024

Abstract Context Previous reviews have focused on the effects of probiotics colitis, but there is a need to understand their impact barrier integrity and tight junction protein improvement in colitis. Objective This study aimed systematically examine probiotic use colitis disease. was conducted according Preferred Reporting Items for Systematic Reviews Meta-Analyses (PRISMA) guidelines. Data Sources A systematic search PubMed, Web Science, Scopus, Cochrane databases identified 2537 articles. Extraction As result search, articles were accessed. Study results summarized descriptively through discussions by intervention conditions, population, measurement methods, key findings. The included studies independently reviewed all authors reached consensus quality major findings from Forty-six that met inclusion criteria analyzed within scope review. Results Although primarily utilized Lactobacillaceae family (notably, L casei, reuteri, rhamnosus, plantarum, pentosus) Bifidobacteriaceae B breve, animalis, dentium), other also demonstrated positive proteins. These are attributed production bioactive metabolic compounds, as well short-chain fatty acids, which combat pathogens reduce anti-inflammatory agents. However, it observed these proteins varied depending strain dose. Conclusion beneficial remission inflammatory bowel disease documented. Studies show generally improve intestinal function, factors such dose, duration, bacterial species combinations further clarification. Additionally, comprehensive needed how improved function affects absorption individuals. Review Registration PROSPERO registration no. CRD42023452774.

Language: Английский

---

Anti-Salmonella Defence and Intestinal Homeostatic Maintenance In Vitro of a Consortium Containing Limosilactobacillus fermentum 3872 and Ligilactobacillus salivarius 7247 Strains in Human, Porcine, and Chicken Enterocytes

Antibiotics, Journal Year: 2023, Volume and Issue: 13(1), P. 30 - 30

Published: Dec. 28, 2023

strain 3872 (LF3872) was originally isolated from the breast milk of a healthy woman during lactation and breastfeeding child.

Language: Английский

---

Understanding the Immunomodulatory Effects of Bovine Colostrum: Insights into IL-6/IL-10 Axis-Mediated Inflammatory Control

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: July 28, 2023

Abstract Bovine colostrum (COL), the first milk secreted by lactating cows postpartum, is a rich source of bioactive compounds that exert significant role on survival, growth, and immune development neonatal calves [9,10]. This study investigated immunomodulatory effects COL cytokine production in vitro using Caco-2/THP-1 macrophage co-culture model stimulated with Phorbol 12-myristate 13-acetate (PMA). pretreatment significantly reduced IL-6 induced PMA, while increasing IL-10 production. Further investigations revealed suppressive effect was heat-sensitive associated components higher molecular mass (100 kDa). Moreover, primarily influenced THP-1 macrophages rather than Caco-2 epithelial cells. The were NF-κB activation macrophages. In calf-FMT transplanted C57BL/6 murine model, decreased intestinal permeability, cell infiltration, suppressed during S. typhimurium infection. These results highlight activity bovine its potential therapeutic applications inflammatory disorders. studies are needed to elucidate underlying mechanisms validate findings models. Simple Summary explores properties initial produced cows, novel model. researchers utilized (PMA) investigate indicate enhancing Colostrum demonstrated prospective research necessary corroborate

Language: Английский

---

CELL SURFACE GLYCANS AS AN ADHESION FACTOR FOR EXOSOMAL VESICLES OF TRITRICHOMONAS SPP. TYPE PARASITES

Published: Dec. 9, 2024

Protozoal infection is a serious factor in the decline of reproductive health humans and animals. The example T. vaginalis shows how parasite-host interaction mediated. It known that signaling between cells parasites carried out with help specific connections. These bonds can be represented by glycans lectins. In this work, we examined glycan profile lectin exosomal vesicles intestinal trichomonad Tritrichomonas sp. an epithelial model. cytotoxicity active trophozoites on CaCo-2 tumor cell line was shown, absence cytotoxic effect also demonstrated. We investigated treatment biotinylated lectins found high percentage binding to RCA120, AAL, UEA-1. bind galactose fucose-containing glycans. When determined chips, bound alpha-Gal beta-Gal GalNAc, as well Fuca1. hypothesize interact host through these glycan-lectin chains. By selecting blockers interactions, parasite adhesion inhibited. mechanisms may form basis new antiprotozoal drugs.

Language: Английский

---