Myco-synthesized copper oxide nanoparticles using harnessing metabolites of endophytic fungal strain Aspergillus terreus: an insight into antibacterial, anti-Candida, biocompatibility, anticancer, and antioxidant activities

BMC Complementary Medicine and Therapies, Journal Year: 2023, Volume and Issue: 23(1)

Published: July 22, 2023

The overuse of antibiotics leads to the emergence antibiotic-resistant microbes which causes high mortality worldwide. Therefore, synthesis new active compounds has multifunctional activities are main challenge. Nanotechnology provides a solution for this issue.The endophytic fungal strain Aspergillus terreus BR.1 was isolated from healthy root Allium sativum and identified using internal transcribed spacer (ITS) sequence analysis. copper oxide nanoparticles (CuO-NPs) were synthesized by harnessing metabolites strain. UV-Visble spectroscopy, Fourier-transform infrared spectroscopy (FT-IR), Transmission electron micrscopy (TEM), Energy dispersive X-ray (EDX), diffraction (XRD), Dynamic light scattering (DLS), zeta potential (ζ) used characterization CuO-NPs. activity against different pathogenic bacteria Candida species investigated agar well-diffusion method. biocombatibility anticancer assessed MTT assay scavenging DPPH investigate antioxidant CuO-NPs.Data showed successful formation crystalline nature spherical shape CuO-NPs with sizes in ranges 15-55 nm. EDX reveals that as-formed sample contains ions C, O, Cl, Cu weight percentages 18.7, 23.82, 11.31, 46.17%, respectively. DLS ζ-potential homogeneity stability polydispersity index (PDI) 0.362 ζ-value - 26.6 mV. exhibited promising antibacterial anti-Candida (concentration-dependent) minimum inhibitory concentration (MIC) values 25-50 µg mL-1. Moreover, mediated-CuO-NPs targeted cancer cells MCF7 PC3 at low IC50 concentrations 159.2 ± 4.5 116.2 3.6 mL-1, respectively as compared normal (Vero Wi38 value 220.6 3.7 229.5 2.1 respectively). biosynthesized detected method 80.5 1.2% 1000 mL-1 decreased 20.4 4.2% 1.9 ascorbic acid (control) 97.3 0.2 37.5 1.3% same concentrations, respectively.The can be integrated into various biomedical theraputic applications.

Language: Английский

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Unveiling the biodeterioration activity of microbial communities to the historical manuscript: Biocontrol using biosynthesized gold and zinc oxide nanoparticles

Journal of Cultural Heritage, Journal Year: 2025, Volume and Issue: 71, P. 440 - 452

Published: Jan. 1, 2025

Language: Английский

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An Eco-Friendly Approach Utilizing Green Synthesized Titanium Dioxide Nanoparticles for Leather Conservation against a Fungal Strain, Penicillium expansum AL1, Involved in the Biodeterioration of a Historical Manuscript

Biology, Journal Year: 2023, Volume and Issue: 12(7), P. 1025 - 1025

Published: July 20, 2023

The main hypothesis of the present research is investigating efficacy titanium oxide nanoparticles (TiO2-NPs) to prevent growth fungal strains when applied on leather under an experimental study. Therefore, fifteen were isolated from a deteriorated historical manuscript (papers and leathers) identified by traditional methods ITS sequence analysis, including Aspergillus chevalieri (one isolate), A. nidulans (two strains), flavus (four cristatus strain), niger Paecilomyces fulvus Penicillium expansum P. citrinum strains). enzymes cellulase, amylase, pectinase, gelatinase, which play crucial role in biodegradation, highly active these strains. TiO2-NPs formed using cell-free filtrate probiotic bacterial strain, Lactobacillus plantarum, characterized. Data showed that successfully with spherical shape anatase phase sizes 2-8 nm. Moreover, EDX analysis revealed Ti O ions occupied component weight percentages 41.66 31.76%, respectively. vitro cytotoxicity toward two normal cell lines, WI38 HFB4, low toxicity effect against cells (IC50 = 114.1 ± 8.1µg mL-1 for Wi38, 237.5 3.5µg HFB4). concentrations 100 μg used load prepared samples before inoculation strain AL1. study loaded have inhibit 73.2 2.5%, 84.2 1.8%, 88.8 0.6% after 7, 14, 21 days, Also, analyses SEM, FTIR-ART, color change, mechanical properties inoculated AL1 absence NPs high damage aspects compared those presence TiO2-NPs.

Language: Английский

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Gut Mycobiome: Latest Findings and Current Knowledge Regarding Its Significance in Human Health and Disease

Journal of Fungi, Journal Year: 2025, Volume and Issue: 11(5), P. 333 - 333

Published: April 22, 2025

The gut mycobiome, the fungal component of microbiota, plays a crucial role in health and disease. Although fungi represent small fraction ecosystem, they influence immune responses, homeostasis, disease progression. mycobiome’s composition varies with age, diet, host factors, its imbalance has been linked to conditions such as inflammatory bowel (IBD) metabolic disorders. Advances sequencing have expanded our understanding fungi, but challenges remain due methodological limitations high variability between individuals. Emerging therapeutic strategies, including antifungals, probiotics, fecal microbiota transplantation, dietary interventions, show promise require further study. This review highlights recent discoveries on interactions bacteria, disease, potential clinical applications. A deeper contributions will help develop targeted microbiome-based therapies.

Language: Английский

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An Insight into Antimicrobial, Antioxidant, Anticancer, and Antidiabetic Activities of Trimetallic Se/ZnO/CuO Nanoalloys Fabricated by Aqueous Extract of Nitraria retusa

Journal of Cluster Science, Journal Year: 2024, Volume and Issue: 36(1)

Published: Dec. 3, 2024

Language: Английский

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Evaluating the efficacy of probiotic bacterial strain Lactobacillus plantarum for inhibition of fungal strains associated with historical manuscript deterioration: an experimental study

Fungal Biology, Journal Year: 2024, Volume and Issue: 128(6), P. 1992 - 2006

Published: July 18, 2024

Language: Английский

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A comprehensive overview of the performance of polyamide 6 in the consolidation of vegetable-tanned leathers

Journal of Cultural Heritage, Journal Year: 2023, Volume and Issue: 64, P. 207 - 215

Published: Nov. 1, 2023

Language: Английский

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Synthesis, Optimization, and Characterization of Cellulase Enzyme Obtained from Thermotolerant Bacillus subtilis F3: An Insight into Cotton Fabric Polishing Activity

Journal of Microbiology and Biotechnology, Journal Year: 2023, Volume and Issue: 34(1), P. 207 - 223

Published: Oct. 18, 2023

Amr Fouda, Khalid S. Alshallash, Hossam M. Atta, Mamdouh El Gamal, Mohamed Bakry, Abdullah Alawam, and Salem Salem. J. Microbiol. Biotechnol. 2024;34:207-23. https://doi.org/10.4014/jmb.2309.09023

Language: Английский

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The Restoration of Mummies in Ancient Egypt

Egyptian Journal of Chemistry, Journal Year: 2023, Volume and Issue: 0(0), P. 0 - 0

Published: July 31, 2023

The human deterioration factor plays a major role in the mummies' deterioration, and it is no less important than other factors, whether burial environment or exposure museums stores.Looting considered one of most serious which led to destruction some mummies during different periods ancient Egypt.This study aims explain impact on mummies, describe its aspects present Egyptian restoration conservation techniques materials used through periods.The Egyptians methods restoring especially reassembling process separated broken parts.Examples were wooden splints such as palm wood splints, bark, reed sticks, fibres from trees, pegs.The resin technique linen with resins also used.The oils mastic, myrrh, beeswax.Different plant fibers wood, linen, fibres, reed, etc.The metal "iron" was this process.This showed that are pioneers world, they preceded others field.It found resistant microorganisms insects, improve mechanical properties mummies.

Language: Английский

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Biopolymer applications for different treatments of the historical paper manuscripts and leather bindings: A Review

Egyptian Journal of Chemistry, Journal Year: 2023, Volume and Issue: 0(0), P. 0 - 0

Published: Sept. 4, 2023

Biopolymers materials exhibit outstanding potential in various conservation applications of historical paper manuscripts and bookbinding due to their flexible functionalization characteristics.This study aims make an integrated for the different application purposes natural synthetic biopolymers that can be used leather bindings.This discussed sources such as plants, animals, microorganism sources.The consolidation, adhesive, retanning, cleaning, gap filling, lubrication, antifungal were explained.The chemical composition, advantages, disadvantages, techniques also discussed.This showed there are many field treatment binding.Still, most common was polysaccharides-based materials, which bio-consolidation paper, this compatibility characteristics with paper.Essential plant oils (tea tree, lavender, thyme oils) Chitosan antimicrobials.Japanese papers bacterial cellulose appropriate consolidation completion missing fragile parts leathers.Polyurethanes, a biomaterial, coating protect from gas pollution.Beeswax protects pollution sulfur dioxide.This is considered guide all conservators working on binding.

Language: Английский

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