Chlamydiae as symbionts of photosynthetic dinoflagellates DOI Creative Commons
Justin Maire, Astrid Collingro, Kshitij Tandon

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 18, 2023

Abstract Chlamydiae are ubiquitous intracellular bacteria and infect a wide diversity of eukaryotes, including mammals. However, chlamydiae have never been reported to photosynthetic organisms. Here, we describe novel chlamydial genus species, Candidatus Algichlamydia australiensis ( A. thereafter), capable infecting the dinoflagellate Cladocopium sp. (originally isolated from scleractinian coral). was confirmed be by fluorescence in situ hybridization confocal laser scanning microscopy, temporally stable at population level monitoring its relative abundance across four weeks host growth. Using combination short- long-read sequencing, recovered high-quality (completeness 91.73% contamination 0.27%) metagenome-assembled genome . Phylogenetic analyses show that this taxon represents new species within Simkaniaceae family. possesses all hallmark genes for chlamydiae-host interactions, complete type III secretion system. In addition, IV system is encoded on plasmid has previously observed only three other species. Twenty orthologous groups unique , one which structurally similar protein known Cyanobacteria Archaeplastida involved thylakoid biogenesis maintenance, hinting potential interactions with chloroplasts cells. Despite being itself symbiont cnidarians, meta-analysis 12,009 cnidarian 16S rRNA gene metabarcoding samples returned five sequences, suggesting does not associate cnidarians. Our study shows symbionts first organism harbor chlamydiae, thereby expanding breadth hosts providing contribution discussion around role establishment primary plastid.

Language: Английский

Coral Skeleton Dwelling Endolithic Algae: Ostreobium and Its Biology DOI
Kshitij Tandon,

Juntong Hu,

Marisa M. Pasella

et al.

Coral reefs of the world, Journal Year: 2025, Volume and Issue: unknown, P. 41 - 46

Published: Jan. 1, 2025

Language: Английский

Citations

1
Microbial Communities in Changing Aquatic Environments DOI Creative Commons
Damir Kapetanović, Mohammad Katouli, Darija Vukić Lušić

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(4), P. 726 - 726

Published: April 3, 2024

The quality of aquatic ecosystems is an important public health concern [...]

Language: Английский

Citations

3
Chlamydiae in corals: shared functional potential despite broad taxonomic diversity DOI Creative Commons
Justin Maire, Astrid Collingro, Matthias Horn

et al.

ISME Communications, Journal Year: 2024, Volume and Issue: 4(1)

Published: Jan. 1, 2024

Abstract Cnidarians, such as corals and sea anemones, associate with a wide range of bacteria that have essential functions, including nutrient cycling the production antimicrobial compounds. Within cnidarians, can colonize all microhabitats tissues. Among them are obligate intracellular phylum Chlamydiota (chlamydiae) whose impact on cnidarian hosts holobionts, especially corals, remain unknown. Here, we conducted meta-analysis previously published 16S rRNA gene metabarcoding data from cnidarians (e.g. coral, jellyfish, anemones), eight metagenome-assembled genomes (MAGs) coral-associated chlamydiae, one MAG jellyfish-associated chlamydiae to decipher their diversity functional potential. While dataset showed an enormous cnidarian-associated six out nine MAGs were affiliated Simkaniaceae family. The other three assigned Parasimkaniaceae, Rhabdochlamydiaceae, Anoxychlamydiaceae, respectively. All lacked genes necessary for independent existence, lacking any nucleotide or vitamin most amino acid biosynthesis pathways. Hallmark chlamydial genes, type III secretion system, transporters, host interaction, encoded in MAGs. Together these observations suggest lifestyle chlamydiae. No unique found suggesting lack specificity. Additional studies needed understand how interact coral host, microbes holobionts. This first study potential improves our understanding both microbiome range.

Language: Английский

Citations

2
Chlamydiae as symbionts of photosynthetic dinoflagellates DOI Creative Commons
Justin Maire, Astrid Collingro, Kshitij Tandon

et al.

The ISME Journal, Journal Year: 2024, Volume and Issue: 18(1)

Published: Jan. 1, 2024

Chlamydiae are ubiquitous intracellular bacteria and infect a wide diversity of eukaryotes, including mammals. However, chlamydiae have never been reported to photosynthetic organisms. Here, we describe novel chlamydial genus species, Candidatus Algichlamydia australiensis, capable infecting the dinoflagellate Cladocopium sp. (originally isolated from scleractinian coral). australiensis was confirmed be by fluorescence in situ hybridization confocal laser scanning microscopy temporally stable at population level monitoring its relative abundance across four weeks host growth. Using combination short- long-read sequencing, recovered high-quality (completeness 91.73% contamination 0.27%) metagenome-assembled genome A. australiensis. Phylogenetic analyses show that this taxon represents new species within Simkaniaceae family. possesses all hallmark genes for chlamydiae-host interactions, complete type III secretion system. In addition, IV system is encoded on plasmid has previously observed only three other species. Twenty orthologous groups unique one which structurally similar protein known Cyanobacteria Archaeplastida involved thylakoid biogenesis maintenance, hinting potential interactions with chloroplasts cells. Our study shows symbionts cnidarians, first organism harbor chlamydiae, thereby expanding breadth hosts providing contribution discussion around role establishment primary plastid.

Language: Английский

Citations

1
Chlamydiae in cnidarians: Shared functional potential despite broad taxonomic diversity DOI Creative Commons
Justin Maire, Astrid Collingro, Matthias Horn

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 20, 2023

Abstract Cnidarians, such as corals and sea anemones, associate with a wide range of bacteria that have essential functions, including nutrient cycling the production antimicrobial compounds. Within cnidarians, can colonize all microhabitats tissues. Among them are obligate intracellular phylum Chlamydiota (chlamydiae) whose impact on cnidarian hosts holobionts remain unknown. Here, we conducted meta-analysis previously published 16S rRNA gene metabarcoding data eight metagenome-assembled genomes (MAGs) cnidarian-associated chlamydiae to decipher their diversity functional potential. While dataset showed an enormous chlamydiae, five out MAGs were affiliated Simkaniaceae family. The other three assigned Parasimkaniaceae, Rhabdochlamydiaceae, Anoxychlamydiaceae, respectively. All associated potential insufficient for independent existence, lacking any nucleotide or vitamin most amino acid biosynthesis pathways. Hallmark chlamydial genes, type III secretion system, transporters, genes host interaction, encoded in MAGs. Together these observations suggest lifestyle chlamydiae. Cnidarian-associated lacked unique suggesting core genetic arsenal may be flexible enough infect many eukaryotic hosts, cnidarians. Additional studies needed understand how interact host, microbes holobionts. This first study improves our understanding both microbiome range.

Language: Английский

Citations

1
Chlamydiae as symbionts of photosynthetic dinoflagellates DOI Creative Commons
Justin Maire, Astrid Collingro, Kshitij Tandon

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 18, 2023

Abstract Chlamydiae are ubiquitous intracellular bacteria and infect a wide diversity of eukaryotes, including mammals. However, chlamydiae have never been reported to photosynthetic organisms. Here, we describe novel chlamydial genus species, Candidatus Algichlamydia australiensis ( A. thereafter), capable infecting the dinoflagellate Cladocopium sp. (originally isolated from scleractinian coral). was confirmed be by fluorescence in situ hybridization confocal laser scanning microscopy, temporally stable at population level monitoring its relative abundance across four weeks host growth. Using combination short- long-read sequencing, recovered high-quality (completeness 91.73% contamination 0.27%) metagenome-assembled genome . Phylogenetic analyses show that this taxon represents new species within Simkaniaceae family. possesses all hallmark genes for chlamydiae-host interactions, complete type III secretion system. In addition, IV system is encoded on plasmid has previously observed only three other species. Twenty orthologous groups unique , one which structurally similar protein known Cyanobacteria Archaeplastida involved thylakoid biogenesis maintenance, hinting potential interactions with chloroplasts cells. Despite being itself symbiont cnidarians, meta-analysis 12,009 cnidarian 16S rRNA gene metabarcoding samples returned five sequences, suggesting does not associate cnidarians. Our study shows symbionts first organism harbor chlamydiae, thereby expanding breadth hosts providing contribution discussion around role establishment primary plastid.

Language: Английский

Citations

0