Toxicological Assessment of 2-Hydroxychalcone-Mediated Photodynamic Therapy: Comparative In Vitro and In Vivo Approaches DOI Creative Commons
Níura Madalena Bila, Carolina Orlando Vaso,

Jenyffie Araújo Belizário

et al.

Pharmaceutics, Journal Year: 2024, Volume and Issue: 16(12), P. 1523 - 1523

Published: Nov. 26, 2024

Background: Photodynamic therapy (PDT) is a treatment modality that uses light to activate photosensitizing agent, destroying target cells. The growing awareness of the necessity reduce or eliminate use mammals in research has prompted search for safer toxicity testing models aligned with new global guidelines and compliant relevant regulations. Objective: objective this study was assess impact PDT on alternative mammals, including vitro three-dimensional (3D) cultures vivo, invertebrate animals, utilizing potent photosensitizer, 2-hydroxychalcone. Methods: Cytotoxicity assessed two cellular models: monolayer (2D) 3D. For purpose, spheroids cell lines, primary dermal fibroblasts (HDFa) adult human epidermal keratinocytes (HaCat), were developed characterized following criteria viability, shape, diameter, number survival percentages Caenorhabditis elegans Galleria mellonella evaluated at 1 7 days, respectively. Results: findings indicated all platforms are appropriate investigating toxicity. Furthermore, 2-hydroxychalcone demonstrated low absence when mediated by across range (2D 3D cultures) vivo (invertebrate animal models, G. C. elegans) models. Conclusion: There strong correlation between tests, similar results, particularly elegans, where concentration 50% viability approximately 100 µg/mL.

Language: Английский

Immunophenotyping of hemocytes from infected Galleria mellonella larvae as an innovative tool for immune profiling, infection studies and drug screening DOI Creative Commons
M. Gallorini, Beatrice Marinacci, Benedetta Pellegrini

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Jan. 8, 2024

Abstract In recent years, there has been a considerable increasing interest in the use of greater wax moth Galleria mellonella as an animal model. vivo pharmacological tests, concerning efficacy and toxicity novel compounds are typically performed mammalian models. However, latter is costly, laborious requires ethical approval. this context, G. larvae can be considered valid option due to their ease absence rules. Furthermore, it demonstrated that immune system these invertebrates similarity with one mammals, thus guaranteeing reliability model, mainly microbiological field. To better develop full potential we present approach characterize hemocyte population from highlight immuno modulation upon infection treatments. Our based on detection isolated hemocytes hemolymph cell membrane markers expressed by human cells inflammation infection, for instance CD14, CD44, CD80, CD163 CD200. This method highlights analogies between humans. provide innovative tool perform pre-clinical evaluations antimicrobial further proceed clinical trials support drug discovery campaigns.

Language: Английский

Citations

9
In Vivo and in Silico Evaluation of the Effect of p-Acetamide and MPAEMA on the Model Organism Galleria Mellonella (Lepidoptera: Pyralidae) DOI Open Access
Nevin Çankaya, Serap Yalçın Azarkan, Fahriye Ercan

et al.

Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, Journal Year: 2025, Volume and Issue: 28(3), P. 625 - 635

Published: May 1, 2025

In this study, 2-chloro-N-(4-methoxyphenyl)acetamide (p-acetamide) and 2-(4-methoxyphenylamino)-2-oxoethyl methacrylate (MPAEMA) were resynthesized to evaluate their effect on the agricultural pest Galleria mellonella. The toxicities of p-acetamide MPAEMA against larval stage G. mellonella evaluated concurrently. results indicate that has a lethal insect larvae at lower doses. LC50 doses 873,572 687,355 uM, respectively. These values represent concentrations substances which 50% exposed them are expected die. molecular docking interactions with proteins superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST) analyzed. binding energy between was determined be -6.8 kcal/mol. This suggests may have an inhibitory could further investigated for developing pesticides target enzyme.

Language: Английский

Citations

0
Antibacterial activity and phytochemical screening of Rumex abyssinicus Jacq and Verbascum sinaiticum Benth collected from Debre Markos, northwest Ethiopia DOI Creative Commons

Agumas Lemlemu,

Solomon Yeshanew,

Asmamaw Habtamu

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 21, 2024

Abstract Background The increasing challenge of antibiotic resistance necessitates the need to explore alternative antimicrobial agents derived from natural sources. Rumex abyssinicus Jacq and Verbascum sinaiticum Benth are well-regarded in Ethiopian traditional medicine for their therapeutic potential. This study thus aimed assess antibacterial activity phytochemical screening extracts roots R. leaves V. collected Debre Markos, northwest Ethiopia. Methods Crude were prepared using a 1:10 w/v cold maceration technique with 80% ethanol chloroform as solvents. In vivo, toxicity was assessed Galleria mellonella larvae exposed at concentrations ranging 12.5 100 mg/ml. Antibacterial activities evaluated disc diffusion assays against four strains human pathogenic bacteria 25 200 Minimum Inhibitory Concentration (MIC) Bactericidal (MBC) also determined quantify potency extracts. Phytochemical analysis identified secondary metabolites standard qualitative tests, while chromatographic techniques: thin layer chromatography (TLC), high-performance liquid (HPLC) conducted fractionation, followed by nuclear magnetic resonance spectroscopic (NMR) characterization, mass spectrometry (MS) isolated compounds. Results Toxicity assessments plant on G. indicated dose-dependent toxic effects, mg/ml causing 40% mortality, suggesting moderate toxicity. Yet, mortality decreased lower concentrations. Both demonstrated significant (p < 0.05) all tested bacteria. extract exhibited highest Staphylococcus aureus (inhibition zone: 21.3 ± 1.21 mm) However, did not exhibit response 50 MIC values consistent 32 across majority bacteria, showed higher (64 mg/ml), indicating potency. revealed presence anthraquinones, saponins, tannins abyssinicus, flavonoids phenols sinaiticum. addition, separation yielded compounds such chrysophanol emodin luteolin aucubin Conclusion Ethanol potent pathogens, supported diverse profiles. These findings underscore potential sources agents, warranting further investigation into applications.

Language: Английский

Citations

1
Toxicological Assessment of 2-Hydroxychalcone-Mediated Photodynamic Therapy: Comparative In Vitro and In Vivo Approaches DOI Creative Commons
Níura Madalena Bila, Carolina Orlando Vaso,

Jenyffie Araújo Belizário

et al.

Pharmaceutics, Journal Year: 2024, Volume and Issue: 16(12), P. 1523 - 1523

Published: Nov. 26, 2024

Background: Photodynamic therapy (PDT) is a treatment modality that uses light to activate photosensitizing agent, destroying target cells. The growing awareness of the necessity reduce or eliminate use mammals in research has prompted search for safer toxicity testing models aligned with new global guidelines and compliant relevant regulations. Objective: objective this study was assess impact PDT on alternative mammals, including vitro three-dimensional (3D) cultures vivo, invertebrate animals, utilizing potent photosensitizer, 2-hydroxychalcone. Methods: Cytotoxicity assessed two cellular models: monolayer (2D) 3D. For purpose, spheroids cell lines, primary dermal fibroblasts (HDFa) adult human epidermal keratinocytes (HaCat), were developed characterized following criteria viability, shape, diameter, number survival percentages Caenorhabditis elegans Galleria mellonella evaluated at 1 7 days, respectively. Results: findings indicated all platforms are appropriate investigating toxicity. Furthermore, 2-hydroxychalcone demonstrated low absence when mediated by across range (2D 3D cultures) vivo (invertebrate animal models, G. C. elegans) models. Conclusion: There strong correlation between tests, similar results, particularly elegans, where concentration 50% viability approximately 100 µg/mL.

Language: Английский

Citations

0