Influence of gut microbiota on Campylobacter colonization and antimicrobial resistance development in the chicken host DOI Open Access
Jinji Pang

Published: Jan. 1, 2023

Campylobacter, predominantly Campylobacter jejuni, is a global foodborne and zoonotic pathogen responsible for significant portion of the gastroenteritis diarrheal illness in humans worldwide. Antibiotic-resistant also recognized as serious threat to public health because it limits treatment options increases severity infections. Contaminated poultry meat has been identified most common source infection many countries, including United States. Therefore, effective control at farm level would greatly reduce spread through food supply chain. In this project, we deciphered association between colonization gut microbiota composition commercial broilers. We evaluated efficacy using fecal transplantation (FMT) potential method reducing Additionally, assessed impact treating broilers with enrofloxacin (ENRO) on metabolic processes. The key findings project can be summarized follows. 1) Significant differences microbial diversity were observed cecal structure Campylobacter-negative Campylobacter-positive broiler flocks over multiple production cycles. 2) FMT reduced significantly when challenge was given individual birds via oral gavage, but not done horizontally by use seeder birds. 3) ENRO initially quickly selected fluoroquinolone (FQ)-resistant mutants treated 4) Both treatments altered significantly; additionally, had substantial metabolism. conclusion, these results showed that differed based their status under settings could substantially influence development FQ-resistance chicken intestine laboratory conditions. These provide valuable insights developing microbiota-based approaches may used mitigate broilers, thereby enhancing safety transmission from humans.

Language: Английский

Chicken caecal enterotypes in indigenous Kadaknath and commercial Cobb chicken lines are associated with Campylobacter abundance and influenced by farming practices DOI Creative Commons
Melanie Hay, Ankit Hinsu, Prakash Koringa

et al.

Frontiers in Microbiomes, Journal Year: 2023, Volume and Issue: 2

Published: Dec. 4, 2023

Identifying farming practices that decrease susceptibility to infectious diseases and optimise food conversion efficiency is valuable for chicken welfare productivity, the environment, public health. Enterotypes can be used define microbial community phenotypes have differential, potentially significant impacts on gut In this study, we delineated enterotypes by analysing microbiomes of 300 indigenous Kadaknath commercial Cobb400 broiler chickens raised across 60 farms in western India. Using a compositional data approach, identified three distinct enterotypes: PA1 (n=290), PA2 (n=142) PA3 (n=67). clustered more closely with each other than PA3, however, had significantly lower alpha diversity PA1. high Firmicutes: Bacteroides ratio, was dominated Faecalibacterium higher abundance Prevotellamassilia enterotypes. characterised its low diversity, common taxa Phascolarctobacterium A Phocaeicola dorei Campylobacter highest Bacteroidota defined prevalence such as CAG-831 Mucispirillum schaedleri . Network analysis showed all different proportions competing Firmicutes-dominant Bacteroidota-dominant guilds. Random Forest Modelling using farm characteristics predictive enterotype. Factors affecting enterotype include whether were open, enclosed or caged, location farms, visitors allowed inside, number people contact chickens, line, presence dogs flock thinning took place. This study suggests are influenced practices, hence modification could reduce burden zoonotic pathogens

Language: Английский

Citations

7
Animal and In Vitro Models as Powerful Tools to Decipher the Effects of Enteric Pathogens on the Human Gut Microbiota DOI Creative Commons
Marco Calvigioni, Diletta Mazzantini, Francesco Celandroni

et al.

Microorganisms, Journal Year: 2023, Volume and Issue: 12(1), P. 67 - 67

Published: Dec. 29, 2023

Examining the interplay between intestinal pathogens and gut microbiota is crucial to fully comprehend pathogenic role of enteropathogens their broader impact on human health. Valid alternatives studies have been introduced in laboratory practice evaluate effects infectious agents microbiota, thereby exploring translational implications functionality overall Different animal species are currently used as valuable models for infections. In addition, considering recent advances bioengineering, futuristic vitro resembling environment also available this purpose. review, main (i.e., Clostridioides difficile, Campylobacter jejuni, diarrheagenic Escherichia coli, non-typhoidal Salmonella enterica, Shigella flexneri sonnei, Vibrio cholerae, Bacillus cereus) microbial communities summarized, with specific emphasis results derived from investigations employing models.

Language: Английский

Citations

4
Campylobacteriosis: A rising threat in foodborne illnesses DOI Creative Commons
Aswin Rafif Khairullah, Sheila Marty Yanestria, Mustofa Helmi Effendi

et al.

Open Veterinary Journal, Journal Year: 2024, Volume and Issue: 14(8), P. 1733 - 1733

Published: Jan. 1, 2024

Campylobacteriosis is a foodborne illness that contracted by eating contaminated food, particularly animal products like meat from diseased animals or corpses tainted with harmful germs. The epidemiology of campylobacteriosis varies significantly between low-, middle-, and high-income countries. Campylobacter has complicated poorly known survival strategy for getting past host barriers causing sickness in humans. adaptability to unfavorable environments the host’s immune system seems be one most crucial elements intestinal colonization. A infection may result fever, nausea, vomiting, mild severe bloody diarrhea Effective rapid diagnosis species infections hosts essential both individual treatment disease management at farm level. According recent meta-analysis research, main risk factor travel, which followed undercooked chicken, being exposed environment, coming into close contact livestock. jejuni, occasionally coli, are primary causes gastroenteritis, significant humans public health. best antibiotic medications eradicating decreasing feces erythromycin, clarithromycin, azithromycin. reduce number human caused restrict amount contamination poultry flock its products, even if majority through handling ingestion chicken.

Language: Английский

Citations

1
The Interactive Effects of Nutrient Density and Breed on Growth Performance and Gut Microbiota in Broilers DOI Creative Commons

Meiting Jia,

Jiaqi Lei, Yuanyang Dong

et al.

Animals, Journal Year: 2024, Volume and Issue: 14(23), P. 3528 - 3528

Published: Dec. 6, 2024

This study investigated whether variations in growth response to low nutrient density across breeds are linked microbiota regulation. Arbor Acres (

Language: Английский

Citations

1
Fecal Microbiota Transplantation Reduces Campylobacter jejuni Colonization in Young Broiler Chickens Challenged by Oral Gavage but Not by Seeder Birds DOI Creative Commons
Jinji Pang, Ashenafi Feyisa Beyi, Torey Looft

et al.

Antibiotics, Journal Year: 2023, Volume and Issue: 12(10), P. 1503 - 1503

Published: Oct. 2, 2023

Campylobacter spp., particularly C. jejuni and coli, are major food safety concerns, transmitted to humans mainly via contaminated poultry meat. In a previous study, we found that some commercial broiler farms consistently produced Campylobacter-free flocks while others reared Campylobacter-colonized flocks, significant differences in the gut microbiota compositions between two types of farm categories were revealed. Therefore, hypothesized influences colonization from may confer resistance chicken intestine. this fecal transplantation (FMT) trials performed test hypothesis. Newly hatched chicks given FMT oral gavage cecal content adult chickens (treatment groups) or PBS (control before feed consumption. Approximately weeks after FMT, birds challenged with either by (trial 1) co-mingling seeder 2) evaluate potential protective effect FMT. Cecal contents collected (3 times, 5 days apart) determine levels culture 16S rRNA gene sequencing. reduced significantly (log10 1.2–2.54 CFU/g) trial 1 but not 2, although impacted diversity both trials. Several genera, such as Butyricimonas, Parabacteroides, Parasutterella, Bilophila, Fournierella, Phascolarctobacterium, Helicobacter, had increased abundance FMT-treated groups Furthermore, was be negatively correlated Escherichia Ruminococcus_torques_group genera. These findings indicate even though can positively affect subsequent development young broilers, its inhibitory on varies appears influenced challenge models.

Language: Английский

Citations

3
No colonization resistance to Campylobacter jejuni in broilers fed brown algal extract-supplemented diets DOI Creative Commons

Eliška Eliasson,

Li Sun, Gunnar Cervin

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: June 27, 2024

gastroenteritis is the most commonly reported zoonosis within EU, with poultry products regarded as primary source of transmission to humans. Therefore, finding strategies reduce

Language: Английский

Citations

0
Influence of gut microbiota on Campylobacter colonization and antimicrobial resistance development in the chicken host DOI Open Access
Jinji Pang

Published: Jan. 1, 2023

Campylobacter, predominantly Campylobacter jejuni, is a global foodborne and zoonotic pathogen responsible for significant portion of the gastroenteritis diarrheal illness in humans worldwide. Antibiotic-resistant also recognized as serious threat to public health because it limits treatment options increases severity infections. Contaminated poultry meat has been identified most common source infection many countries, including United States. Therefore, effective control at farm level would greatly reduce spread through food supply chain. In this project, we deciphered association between colonization gut microbiota composition commercial broilers. We evaluated efficacy using fecal transplantation (FMT) potential method reducing Additionally, assessed impact treating broilers with enrofloxacin (ENRO) on metabolic processes. The key findings project can be summarized follows. 1) Significant differences microbial diversity were observed cecal structure Campylobacter-negative Campylobacter-positive broiler flocks over multiple production cycles. 2) FMT reduced significantly when challenge was given individual birds via oral gavage, but not done horizontally by use seeder birds. 3) ENRO initially quickly selected fluoroquinolone (FQ)-resistant mutants treated 4) Both treatments altered significantly; additionally, had substantial metabolism. conclusion, these results showed that differed based their status under settings could substantially influence development FQ-resistance chicken intestine laboratory conditions. These provide valuable insights developing microbiota-based approaches may used mitigate broilers, thereby enhancing safety transmission from humans.

Language: Английский

Citations

0