Bacillus velezensis LY7 promotes pepper growth and induces resistance to Colletotrichum scovillei

Biological Control, Journal Year: 2024, Volume and Issue: 192, P. 105480 - 105480

Published: March 7, 2024

Anthracnose of pepper (Capsicum annuum L), caused by Colletotrichum scovillei, is one the three major diseases that seriously impact yield and quality. As an alternative to use synthetic chemical pesticides, a variety endophytic bacteria have been reported be potential biocontrol agents with ability improve disease resistance abiotic stress tolerance in host plants. However, mechanisms action which these different bacterial inhibit onset development disease, some cases, remains unclear. In present study, strain Bacillus velezensis LY7, originally isolated from leaves, as agent was evaluated. More specifically, LY7 control anthracnose promote growth assessed pot experiments greenhouse vitro assays. Results indicated B. enhanced synthesis jasmonic acid other hormones plants, well increased plant growth. A 200 × dilution fermentation broth reduced incidence up 90.71 %, while also enhancing The mechanism secreted antibiotic protein possessing cupin domain OS. RNA-sequence analysis transcriptome C. scovillei at several timepoints after exposure (24, 48, 72 h) revealed up- down-regulation genes. induced expression genes associated biosynthesis pathways transcription factors involved pathogenicity. Our results indicate induces hormone plants promotes growth, enhances antioxidant defense-related enzyme activity. These demonstrate using for

Language: Английский

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Temporal dynamics of walnut phyllosphere microbiota under synergistic pathogen exposure and environmental perturbation

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: April 1, 2025

Phyllosphere-associated microbes directly influence plant-pathogen interactions, and the external environment plant shape phyllosphere microbiome. In this study, we integrated 16S rRNA ITS high-throughput sequencing to systematically investigate changes in microbiome between symptomatic asymptomatic walnut leaves affected by spot disease, with consideration of phenological stage progression. Additionally, explored how abiotic (AT, DT, SCTCC & LPDD) biotic factors (Pn Gs) impact microbial communities. Our findings revealed significant differences diversity at same stage. Furthermore, structure function phyllosphere-associated changed as progressed. Fungal taxa that related Plant_Pathogen bacterial KEGG pathway functions Fatty acid biosynthesis Biotin metabolism were increased group. The keystone species driving was Pseudomonas spp., which substantially influenced vs. leaves. Notably, spp. interacted Xanthomonas Pantoea Correlation analysis dew point temperature constituted primary factor community composition, whereas liquid precipitation depth dimension identified dominant shaping fungal taxa. leaf net photosynthetic rate stomatal conductance closely linked These results advance our understanding community-level responses pathogen invasion highlight multifactorial drivers assembly. Ultimately, they contribute predicting managing leaf-related diseases.

Language: Английский

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A dual RNA-seq analyses revealed dynamic arms race during the invasion of walnut by Colletotrichum gloeosporioides

BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)

Published: July 10, 2024

Walnut anthracnose caused by Colletotrichum gloeosporioides seriously endangers the yield and quality of walnut, has now become a catastrophic disease in walnut industry. Therefore, understanding both pathogen invasion mechanisms host response processes is crucial to defense against C. infection.

Language: Английский

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Metabolic and Antioxidant Responses of Different Control Methods to the Interaction of Sorghum sudangrass hybrids-Colletotrichum boninense

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(17), P. 9505 - 9505

Published: Aug. 31, 2024

Colletotrichum boninense is the main pathogenic fungus causing leaf spot disease in Sorghum sudangrass hybrids, which seriously impairs its quality and yield. In order to find an efficient green means of control, this study used agar disk diffusion method screen for a fungicide with strongest inhibitory effect on C. from among several bacteria, fungi, chemicals. Then, changes plant’s antioxidant system metabolic levels after treatment were compare three control. The lowest concentration Zalfexam was 10 mg/mL, at point did not grow, inhibition rates Bacillus velezensis (X7) Trichoderma harzianum 33.87–51.85% 77.86–80.56%, respectively. Superoxide dismutase (SOD) chitinase up-regulated 2.43 1.24 folds group (M group) SOD activity 2.2 (X7 compared control (CK group). SOD, peroxidase (POD), activities elevated (HX differential metabolites different groups mainly enriched amino acid metabolism production, flavonoid lipid pathways. Compared diseased plants (ZB group), M, X7, HX, CK co-enriched tryptophan pathway glutamate–arginine pathway, only showed down-regulation two common pathways, while pathways HX groups. addition, salicylic acid–jasmonic ascorbic acid–glutathione, unique M group, played important role helping hybrids acquire systemic resistance against stress. This fills gap boninene, causes hybrids. paper represents first reported case biological provides reference as well other crops infected boninense.

Language: Английский

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Transcriptome Analysis and Validation of Anthracnose Resistance Genes in Walnut Varieties

Agronomy, Journal Year: 2024, Volume and Issue: 14(5), P. 911 - 911

Published: April 26, 2024

Anthracnose is extremely detrimental to walnut production, because it seriously affects its yield. Therefore, this study aimed identify anthracnose resistance genes in walnuts, lay the foundation for cultivating novel anthracnose-resistant varieties. In study, fruits of varieties “Qinyou 1” and 2”, exhibiting relatively weak anthracnose, were used transcriptome sequencing identifying differentially expressed (DEGs). Thereafter, we a quantitative real-time polymerase chain reaction verify reliability data using Qinyou 1, 2, 3, Liaohe Diamond 1. We identified 6326 DEGs post-infection which 3065 upregulated 3261 downregulated. also found 2055 (969 1086 downregulated genes) two varieties, after 6 days infection. Based on GO KEGG enrichment analyses data, noted that primarily involved metabolic processes, cell composition, structure, catalytic activity, binding activity. Furthermore, functional analysis showed amino acid biosynthesis pathway plant–pathogen interaction may be activated under expression levels different significantly different. Thus, findings provide important genetic resources molecular markers cultivation

Language: Английский

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A Test for Bacillus Spp. Ability to Suppress Pestalotiopsis sp. Causing Rubber Leaf Drought Through In-Vitro

JURNAL AGRONOMI TANAMAN TROPIKA (JUATIKA), Journal Year: 2024, Volume and Issue: 6(2)

Published: May 31, 2024

Using synthetic chemical pesticides can cause environmental pollution, the death of biological agents, emergence residues, and is dangerous for humans. One environmentally friendly alternative disease control using Bacillus spp. This study aimed to test antagonistic ability spp., obtain a morphological description Pestalotiopsis sp. get type bacteria that inhibit fungus causes rubber leaf fall in vitro. research was carried out at Plant Disease Laboratory, Faculty Agriculture, Riau University Pekanbaru Class 1 Quarantine Laboratory. experimentally completely randomized design (CRD) consisting 7 treatments 4 replications. spp used namely (B0) Without (B1) amyloliquefaciens, (B2) cereus, (B3) pseudomycoides, (B4) velezensis 1, (B5) 2, (B6) 3. The data obtained from results were analyzed statistically variance analysis further tested DNMRT Test 5% level. All are able sp., but 2 Bacillus, B. cereus not produce an inhibition zone. amyloliquefaciens more capable inhibiting with inhibitory power 69.85% zone 64.74% reduce length width conidia.

Language: Английский

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Combined transcriptomic and metabolomic analysis of the mechanism by which Bacillus velezensis induces resistance to anthracnose in walnut

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Oct. 9, 2024

Walnut ( Juglans spp.), a significant deciduous tree of economic and ecological importance, faces substantial threats from walnut anthracnose, primarily caused by Colletotrichum gloeosporioides . Bacillus velezensis has shown promise in mitigating this fungal pathogen. To delve deeper into the induction mechanism B. on plant resistance, we conducted metabolomic analysis leaves six different treatment groups. Specifically, groups were defined as follows: Group B.v. was inoculated with alone, CK served blank control, C.g. solely C. B.v.−C.g. received followed inoculation. B.v.+C.g. underwent simultaneous inoculation both , while C.g.−B.v. treated first then A total 1,503 metabolites detected, mainly including flavonoids, terpenoids, steroids. The results revealed that spraying not only enhanced inherent resistance plants but also significantly regulated already infected This achieved inducing to adjust their metabolic pathways such salicylic acid, jasmonic abscisic thereby strengthening stress response. Transcriptomic correlation analyses showed comparisons vs. CK, C.g., 59, 244, 122 differential abundance along 7860, 3677, 5587 genes, respectively. Amino acid synthesis, starch sucrose metabolism, photosynthesis, phenylpropane purine glutathione metabolism played crucial roles walnut’s disease mechanism. Further induced regulate multiple LOC109005403 LOC108985444 LOC118344177 resulting production defensive palmitic coumarin ferulic enhancing In summary, induces systemic modulating acid. It enhances cell walls, synthesizing secondary metabolites, regulating energy responses. These findings provide solid theoretical foundation for future field application controlling anthracnose.

Language: Английский

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Bacillus Velezensis Ly7 Promotes Pepper Growth and Induces Resistance Against the Anthracnose Fungus Colletotrichum Scovillei

Published: Jan. 1, 2023

Pepper anthracnose is one of the three major diseases pepper (Capsicum annuum L.) crops, which seriously affects yield and quality production. As an alternative to chemical pesticides, endophytic bacteria have received increased attention as potential biocontrol agents with ability improve plant resistance stress tolerance. However, mechanisms action remain unclear. In this study, we evaluated applicability bacterial strain Bacillus velezensis LY7 isolated from leaves a in agriculture. We determined control caused by Colletotrichum scovillei promote growth using pot experiments greenhouse vitro assays. This due inoculation B. will make plants jasmonic acid, water acid other endogenous hormones increased, promoting while enhancing resistance. The protection effect fermentation broth diluted 200 times reached up 90.71%, had potent growth-promoting on healthy plants. further mechanism LY7. Strain secreted antibacterial protein, was identified rare cupin domain-containing protein OS. RNA-sequencing analysis revealed that after 24, 48, 72 h, genes were varying degrees up-regulated down-regulated. particular, induced expression related biosynthesis pathways transcription factors involved Our results suggest induces production simultaneously tolerance antioxidant defence enzymes, demonstrating its practical application safe environmentally friendly agent for anthracnose.

Language: Английский

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