Genetic profile of carbapenem-resistant <i>Acinetobacter baumannii</i> strains DOI Creative Commons
А. Е. Алексеева, Н. Ф. Бруснигина, M. A. Makhova

et al.

Russian Journal of Infection and Immunity, Journal Year: 2024, Volume and Issue: 14(4), P. 681 - 689

Published: Oct. 30, 2024

The purpose of the research: molecular genetic characteristic ofA. baumanniicarbapenem-resistant strains, including clonal affiliation, resistome and virulome pattern analysis, description environment resistance determinants, comparative assessment phylogenetic relationships are presented in work. studiedA. baumanniistrains belonged to sequence types ST2Pas/ST2062.2063Oxf(n = 5) ST78Pas/ST1757Oxf(n 2). nucleotide sequences baumanniiST2Pas/ST2062.2063Oxfstrains were grouped into a single cluster according analysis. And theA. baumanniiST78Pas/ST1757Oxfstrains combined with baumanniiAbCTX5 strain, isolated France 2015. presence intrinsic (OXA-51-like) acquired carbapenemases genes was shown inA. baumanniistrains. In particular,blaOXA-23are identified members ST2Pas/ST2062.2063Oxf, —blaOXA-72as part plasmid DNA. TheA. baumanniiST78Passtrains possessed additional extended-spectrum beta-lactamase genes. Тhe CTX-M-115 cephalosporinase gene present both baumanniistrain NNAb_2023.3 has theblaCARB-16gene. MostA. characterized by for enzymatic modification macrolides(mph(E), msr(E)), chloramphenicols(catB8), aminoglycosides(aadA, aph(3'')-Ib, aph(6)-Id, aac(6')-Ib, aph(3')-Ia, armA). A analysis showed that determinants macrolides aminoglycosides located Tn6279-like transposon, aminoglycosidases genesaph(3'')-Ib, aph(6)-Idare associated theIS91-like mobile element. InA. baumanniiST78Pas/ST1757Oxfstrains, aminoglycosides, macrolides, sulfonamides, region from 60 80 Kb long between theglmSandhutCgenes. mutations thegyrAandparCgenes fluoroquinolones allA. Thus, new knowledge about profile carbapenem-resistantA. representing epidemically significant international lineage been obtained.

Language: Английский

A multiplex RPA coupled with CRISPR-Cas12a system for rapid and cost-effective identification of carbapenem-resistant Acinetobacter baumannii DOI Creative Commons

Zihan Zhou,

Lina Liang,

Chuan Liao

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: March 6, 2024

Background Carbapenem-resistant Acinetobacter baumannii (CRAB) poses a severe nosocomial threat, prompting need for efficient detection methods. Traditional approaches, such as bacterial culture and PCR, are time-consuming cumbersome. The CRISPR-based gene editing system offered potential approach point-of-care testing of CRAB. Methods We integrated recombinase polymerase amplification (RPA) CRISPR-Cas12a to swiftly diagnose CRAB-associated genes, OXA-51 OXA-23 . This multiplex RPA-CRISPR-Cas12a eliminates bulky instruments, ensuring simplified UV lamp-based outcome interpretation. Results Operating at 37°C 40°C, the entire process achieves CRAB diagnosis within 90 minutes. Detection limits genes 1.3 × 10 −6 ng/μL, exhibiting exclusive without cross-reactivity common pathogens. Notably, platform shows 100% concordance with PCR when 30 clinical strains. Conclusion In conclusion, our RPA coupled provides fast sensitive method, overcoming limitations traditional approaches holding promise testing.

Language: Английский

Citations

7
New Antibiotics for the Treatment of Nosocomial Central Nervous System Infections DOI Creative Commons
Roland Nau, Jana Seele,

Helmut Eiffert

et al.

Antibiotics, Journal Year: 2024, Volume and Issue: 13(1), P. 58 - 58

Published: Jan. 7, 2024

Nosocomial central nervous system (CNS) infections with carbapenem- and colistin-resistant Gram-negative vancomycin-resistant Gram-positive bacteria are an increasing therapeutic challenge. Here, we review pharmacokinetic pharmacodynamic data clinical experiences new antibiotics administered intravenously for the treatment of CNS by multi-resistant bacteria. Cefiderocol, a siderophore extended-spectrum cephalosporin, pharmacokinetically behaves similar to established cephalosporins at high doses will probably be valuable addition in our armamentarium infections. The glycopeptides dalbavancin, telavancin, oritavancin highly bound plasma proteins. Although effective animal models meningitis, it is unlikely that they reach cerebrospinal fluid (CSF) concentrations after intravenous administration alone. β-lactam/β-lactamase inhibitor combinations have principal problem both compounds must achieve adequate CSF concentrations. In commercially available combinations, dose β-lactamase tends too low oxazolidinone tedizolid has broader spectrum but less suitable profile than linezolid. halogenated tetracycline eravacycline does not sufficient treat usual dosing. Generally, should intravenous, whenever possible, avoid adverse effects intraventricular therapy (IVT). An additional IVT can overcome limited penetration many into CSF. It considered patients which infection responds poorly systemic antimicrobial

Language: Английский

Citations

6
Bacteriophage and Phage-Encoded Depolymerase Exhibit Antibacterial Activity Against K9-Type Acinetobacter baumannii in Mouse Sepsis and Burn Skin Infection Models DOI Creative Commons
Alexander I. Borzilov, Nikolay V. Volozhantsev,

Olga V. Korobova

et al.

Viruses, Journal Year: 2025, Volume and Issue: 17(1), P. 70 - 70

Published: Jan. 6, 2025

Acinetobacter baumannii is a widely distributed nosocomial pathogen that causes various acute and chronic infections, particularly in immunocompromised patients. In this study, the activities of K9-specific virulent phage AM24 phage-encoded depolymerase DepAPK09 were assessed using vivo mouse sepsis burn skin infection models. model, case prevention or early treatment, single recombinant injection was able to protect 100% mice after parenteral with lethal dose A. K9-type, complete eradication pathogen. delayed survival decreased 70% when injected 40% treated enzyme. number cells on surface wound deep layers by several-fold treatment both depolymerase. The highly stable retained activity under wide range temperatures pH values. results obtained contribute expanding our understanding therapeutic potential specific phages phage-derived depolymerases interacting different capsular types.

Language: Английский

Citations

0
Inhibitory Effect of Antimicrobial Peptides Bac7(17), PAsmr5-17 and PAβN on Bacterial Growth and Biofilm Formation of Multidrug-Resistant Acinetobacter baumannii DOI Creative Commons

Johanna Rühl-Teichner,

Daniela Müller,

Ivonne Stamm

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(3), P. 639 - 639

Published: March 11, 2025

Acinetobacter (A.) baumannii is a major nosocomial pathogen in human and veterinary medicine. The emergence of certain international clones (ICs), often with multidrug-resistant (MDR) phenotypes biofilm formation (BF), facilitates its spread clinical environments. global rise antimicrobial resistance demands alternative treatment strategies, such as peptides (AMPs). In this study, 45 companion animal MDR-A. isolates, belonging to the globally IC1, IC2 IC7, were tested for biofilm-associated genes (BAGs) their capacity BF. Of these, 13 used test inhibitory effect AMPs on bacterial growth (BG) BF through application crystal violet assay. two novel AMP variants Bac7(17) (target cell inactivation) Pasmr5-17 (efflux pump inhibition) well-known phenylalanine-arginine-β-naphthylamide (PAβN) at concentrations 1.95 1000 µg/mL. Based whole-genome sequence data, identical patterns BAGs detected within same IC. inhibited BG dose-dependent manner. PAsmr5-17 highly effective against BG, inhibition (GI) >99% (62.5 125 µg/mL, respectively). PAβN achieved only 95.7% GI Similar results obtained Differences between ICs found both when influenced by AMPs. had hardly any IC1 but IC7 31.25 µg/mL was sufficient. Our data show that susceptibility most likely resembles depending assignment particular Even low significant BG. Therefore, represent promising baumannii, either sole therapy or combination antibiotics.

Language: Английский

Citations

0
Detection of carbapenemase producing Acinetobacter baumannii ST19 from Georgia and Ukraine carrying blaOXA-23, blaOXA-72, and/or blaNDM-5, December 2019 to June 2023 DOI Creative Commons

Ting Luo,

Melissa J. Martin,

Valentyn Kovalchuk

et al.

Eurosurveillance, Journal Year: 2024, Volume and Issue: 29(24)

Published: June 13, 2024

In 2003−2023, amid 5,436 Acinetobacter baumannii isolates collected globally through the Multidrug-Resistant Organism Repository and Surveillance Network, 97 were ST19 PAS , 34 of which carbapenem-resistant. Strains (n = 32) sampled after 2019 harboured either bla OXA-23 OXA-72 and/or NDM-5 . Phylogenetic analysis 11 publicly available genomes revealed three sub-lineages carbapenemase-producing from mainly Ukraine Georgia, including an epidemic clone carrying all carbapenemase genes. Infection control global surveillance carbapenem-resistant A. remain important.

Language: Английский

Citations

2
Pathogenic and Harmful Bacteria in Dairy Technology: Genomic Characterization and Its Correlation with Physicochemical Parameters of Sheep’s Cheese Sold in Southern Brazil DOI Creative Commons
Matheus Abraão Piovesan Pedroso, Wemerson de Castro Oliveira, Andrei Giacchetto Felice

et al.

Fermentation, Journal Year: 2024, Volume and Issue: 10(8), P. 397 - 397

Published: July 31, 2024

The distinct microbiological and physicochemical characteristics of sheep milk enable the production cheeses with unique organoleptic properties. scenario in Brazil is still uncertain, lacking data regulations. This study aims to characterize diversity bacterial groups potential pathogenic and/or harmful properties technology correlate presence these bacteria cheese. Additionally, it investigates virulence genes, resistance toxins reference genomes. main steps were (a) sample preparation fractionation; (b) analysis; (c) analysis free fatty acids profile by GC-FID; (d) volatile compounds GC-MS; (e) molecular analysis—Next Generation Sequencing 16S rRNA gene (V3-V4 region); (f) silico analysis—Genomics. A total 1,061,392 sequences identified, corresponding 45 genera 57 species. Of abundance, 95.7% considered beneficial, while 3.9% classified as (BPP) (BHMT). Five phyla identified: Firmicutes, Proteobacteria, Actinobacteriota, Bacteroidota, Fusobacteriota. Acinetobacter, Pseudomonas, Staphylococcus stood out BPP BHMT groups, showing higher abundance. Alpha cheese samples showed that origin significantly influences richness, evenness, abundance Some parameters, acids, organic a negative correlation Staphylococcus. Reference genomes two species exhibited number genes. reinforces need monitor lesser relevance prevent transmission, through food, potentially resistant virulent pathogens.

Language: Английский

Citations

1
Ventriculitis due to multidrug-resistant gram-negative bacilli associated with external ventricular drain: evolution, treatment, and outcomes DOI Creative Commons
Ana Luisa Corona-Nakamura,

Martha Judith Arias-Merino,

Eleazar Iván Ávila-Esparza

et al.

Frontiers in Neurology, Journal Year: 2024, Volume and Issue: 15

Published: April 26, 2024

Nosocomial infectious ventriculitis caused by multidrug-resistant (MDR) Gram-negative bacilli associated with external ventricular drainage (EVD) placement poses a significant mortality burden and hospital costs.

Language: Английский

Citations

0
Deciphering the impact of Acinetobacter baumannii on human health, and exploration of natural compounds as efflux pump inhibitors to treat multidrug resistance DOI
Karthiga Sivarajan, Ramya Ravindhiran, Jothi Nayaki Sekar

et al.

Journal of Medical Microbiology, Journal Year: 2024, Volume and Issue: 73(8)

Published: Aug. 30, 2024

is an ESKAPE pathogen and threatens human health by generating infections with high fatality rates.

Language: Английский

Citations

0
Genetic profile of carbapenem-resistant <i>Acinetobacter baumannii</i> strains DOI Creative Commons
А. Е. Алексеева, Н. Ф. Бруснигина, M. A. Makhova

et al.

Russian Journal of Infection and Immunity, Journal Year: 2024, Volume and Issue: 14(4), P. 681 - 689

Published: Oct. 30, 2024

The purpose of the research: molecular genetic characteristic ofA. baumanniicarbapenem-resistant strains, including clonal affiliation, resistome and virulome pattern analysis, description environment resistance determinants, comparative assessment phylogenetic relationships are presented in work. studiedA. baumanniistrains belonged to sequence types ST2Pas/ST2062.2063Oxf(n = 5) ST78Pas/ST1757Oxf(n 2). nucleotide sequences baumanniiST2Pas/ST2062.2063Oxfstrains were grouped into a single cluster according analysis. And theA. baumanniiST78Pas/ST1757Oxfstrains combined with baumanniiAbCTX5 strain, isolated France 2015. presence intrinsic (OXA-51-like) acquired carbapenemases genes was shown inA. baumanniistrains. In particular,blaOXA-23are identified members ST2Pas/ST2062.2063Oxf, —blaOXA-72as part plasmid DNA. TheA. baumanniiST78Passtrains possessed additional extended-spectrum beta-lactamase genes. Тhe CTX-M-115 cephalosporinase gene present both baumanniistrain NNAb_2023.3 has theblaCARB-16gene. MostA. characterized by for enzymatic modification macrolides(mph(E), msr(E)), chloramphenicols(catB8), aminoglycosides(aadA, aph(3'')-Ib, aph(6)-Id, aac(6')-Ib, aph(3')-Ia, armA). A analysis showed that determinants macrolides aminoglycosides located Tn6279-like transposon, aminoglycosidases genesaph(3'')-Ib, aph(6)-Idare associated theIS91-like mobile element. InA. baumanniiST78Pas/ST1757Oxfstrains, aminoglycosides, macrolides, sulfonamides, region from 60 80 Kb long between theglmSandhutCgenes. mutations thegyrAandparCgenes fluoroquinolones allA. Thus, new knowledge about profile carbapenem-resistantA. representing epidemically significant international lineage been obtained.

Language: Английский

Citations

0