Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 21, 2023

Abstract Background Prevalent viral particles are found in activated sludge (AS) and anaerobic digester (AD) during wastewater treatment processes. In contrast to the conventional approaches, culture-independent metagenomic method opens up possibility characterize uncultured viruses, which were previously inaccessible. this study, we aimed investigate involvement of viruses AS, mesophilic AD, thermophilic AD using sequences. We constructed viromes, predicted potential host, profiled functional genes genome. Results From a total 64 samples, 42,548 detected. Among them, 43.6% classified Uncultivated Viral Genomes (UViGs) by IMG/VR database 28.5% clustered into clusters (VCs) vConTACT2. total, only 18.2% taxonomically assigned known families, with Siphoviridae , Myoviridae Inoviridae Podoviridae being most commonly observed. Most detected associated engineered ecosystem, more AS environmental ecosystem than AD. Diversity analysis suggested that local habitat has greater impact on viromes immigration effect. Host prediction revealed mostly linked site-specific active populations involved syntrophic fatty acid oxidation, methanogenesis, hydrolysis, fermentation. Functional gene annotation profile follows microbial profile, suggesting not tend infect hosts but also acquire transferred from hosts. Conclusions Overall, study provided insights diversity process uncovered

Language: Английский