Antibacterial Activity and Mechanism of Litsea cubeba Essential Oil Against Salmonella typhimurium DOI Creative Commons

Cuncai Wang,

Xiying Chen,

Mingjie Liu

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(9), P. 1343 - 1343

Published: April 29, 2025

Litsea cubeba essential oil (LCEO) has been reported as an antibacterial agent, but its effects against Salmonella typhimurium (S.Tm) and the underlying mechanisms remain unclear. The efficacy of LCEO was assessed utilizing both microdilution growth curve methodologies, chemical composition thoroughly analyzed. Morphological alterations in cells were observed through scanning electron microscopy (SEM), while cellular permeability gauged based on variations nucleic acid protein contents. impact ATPase activity anti-biofilm formation using colorimetric methods. results indicated that MIC MBC S.Tm 0.4 mg/mL 0.8 mg/mL, respectively. SEM PI staining revealed disrupted bacterial cell integrity. Compared to those control group, treatment with significantly elevated levels extracellular acids proteins (p < 0.05). Furthermore, at MIC, led a 77.9% reduction AKP content, decreased intracellular Na+K+-ATPase Ca2+Mg2+-ATPase activities by 79.9% 54.6%, Additionally, markedly inhibited biofilm formation, enhanced surface hydrophobicity, diminished swimming motility S.Tm. Overall, exhibited promising properties, indicating potential effective inhibitor

Language: Английский

The Unique Capability of Endolysin to Tackle Antibiotic Resistance: Cracking the Barrier DOI Creative Commons

Abdus Sabur,

Ashraful Islam Khan, Bhabesh Borphukan

et al.

Journal of Xenobiotics, Journal Year: 2025, Volume and Issue: 15(1), P. 19 - 19

Published: Jan. 25, 2025

The lack of new antibacterial medicines and the rapid rise in bacterial resistance to antibiotics pose a major threat individuals healthcare systems. Despite availability various antibiotics, has emerged for almost every antibiotic discovered date. increasing prevalence multidrug-resistant strains rendered some infections nearly untreatable, posing severe challenges health care. Thus, development alternatives conventional is critical treatment both humans food-producing animals. Endolysins, which are peptidoglycan hydrolases encoded by bacteriophages, represent promising class antimicrobials. Preliminary research suggests that endolysins more effective against Gram-positive bacteria than Gram-negative when administered exogenously, although they can still damage cell wall bacteria. Numerous have modular domain structure divides their binding catalytic activity into distinct subunits, helps maximize bioengineering potential drug development. Endolysins endolysin-derived antimicrobials offer several advantages as substitutes. They unique mechanism action efficacy persisters (without requiring an active host metabolism); subsequently, target (including antibiotic-resistant strains), mycobacteria. Furthermore, there been limited evidence endolysin being resistant. Because these enzymes highly conserved links, may develop slowly compared traditional antibiotics. This review provides overview insight applications novel

Language: Английский

Citations

1
Medical Device-Associated Healthcare Infections: Sterilization and the Potential of Novel Biological Approaches to Ensure Patient Safety DOI Open Access
Mary Garvey

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 25(1), P. 201 - 201

Published: Dec. 22, 2023

Healthcare-associated infections caused by multi-drug-resistant pathogens are increasing globally, and current antimicrobial options have limited efficacy against these robust species. The WHO details the critically important bacterial fungal species that often associated with medical device HAIs. effective sterilization of devices plays a key role in preventing infectious disease morbidity mortality. A lack adherence to protocol limitations each modality, however, allows for incidence disease. Furthermore, issues relating carcinogenic emissions from ethylene oxide gas (EtO) motivated EPA propose limiting EtO use or seeking alternative methods devices. Food Drug Administration supports healthcare products using low-temperature VH2O2 as an EtO. With advances biomaterial combination products, modalities becoming limited. Novel approaches disinfection devices, biomaterials, therapeutics warranted safeguard public health. Bacteriophages, endolysins, peptides considered promising prophylactic meta-phylactic control diseases. This timely review discusses application biologics agents pathogens, including ESKAPE

Language: Английский

Citations

22
Hospital Acquired Sepsis, Disease Prevalence, and Recent Advances in Sepsis Mitigation DOI Creative Commons
Mary Garvey

Pathogens, Journal Year: 2024, Volume and Issue: 13(6), P. 461 - 461

Published: May 30, 2024

Sepsis is a life-threatening organ dysfunction caused by dysregulated host response to infection, commonly associated with nosocomial transmission. Gram-negative bacterial species are particularly problematic due the release of lipopolysaccharide toxins upon cell death. The toxin E. coli has greater immunogenic potential than that other bacteria. resultant dysregulation immune system failure and mortality, pregnant women, ICU patients, neonates being vulnerable. Additionally, sepsis recovery patients have an increased risk re-hospitalisation, chronic illness, co-morbidities, damage/failure, reduced life expectancy. emergence increasing prevalence antimicrobial resistance in fungal impacted treatment leading mortality rates. Multidrug resistant pathogens including vancomycin-resistant Enterococcus, beta lactam-resistant Klebsiella, carbapenem-resistant Acinetobacter mortality. To improve prognosis predominantly high-risk neonates, advances must be made early diagnosis, triage, control sepsis. identification suitable biomarkers biomarker combinations, coupled machine learning artificial intelligence, show promise detection protocols. Rapid diagnosis essential inform on clinical treatment, especially infectious agents. This timely review aims discuss prevalence, aetiology, recent towards disease mitigation control.

Language: Английский

Citations

6
Three-mode ratiometric biosensor based on integrated DNA-driven magnetic beads for Clostridium perfringens detection DOI
Wenzhuo Wang, Wei Yuan, Tingting Lin

et al.

Food Chemistry, Journal Year: 2024, Volume and Issue: 463, P. 141228 - 141228

Published: Sept. 10, 2024

Language: Английский

Citations

4
Antibacterial Activity and Mechanism of Litsea cubeba Essential Oil Against Salmonella typhimurium DOI Creative Commons

Cuncai Wang,

Xiying Chen,

Mingjie Liu

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(9), P. 1343 - 1343

Published: April 29, 2025

Litsea cubeba essential oil (LCEO) has been reported as an antibacterial agent, but its effects against Salmonella typhimurium (S.Tm) and the underlying mechanisms remain unclear. The efficacy of LCEO was assessed utilizing both microdilution growth curve methodologies, chemical composition thoroughly analyzed. Morphological alterations in cells were observed through scanning electron microscopy (SEM), while cellular permeability gauged based on variations nucleic acid protein contents. impact ATPase activity anti-biofilm formation using colorimetric methods. results indicated that MIC MBC S.Tm 0.4 mg/mL 0.8 mg/mL, respectively. SEM PI staining revealed disrupted bacterial cell integrity. Compared to those control group, treatment with significantly elevated levels extracellular acids proteins (p < 0.05). Furthermore, at MIC, led a 77.9% reduction AKP content, decreased intracellular Na+K+-ATPase Ca2+Mg2+-ATPase activities by 79.9% 54.6%, Additionally, markedly inhibited biofilm formation, enhanced surface hydrophobicity, diminished swimming motility S.Tm. Overall, exhibited promising properties, indicating potential effective inhibitor

Language: Английский

Citations

0