New mouse model for inducible hACE2 expression enables to dissect SARS-CoV-2 pathology beyond the respiratory system DOI Creative Commons

Federica Gambini,

Dominik Arbon, Petr Nickl

et al.

Mammalian Genome, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 22, 2025

The Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) infection is not limited to the respiratory tract as receptors, including angiotensin-converting enzyme 2 (ACE2), are expressed across many tissues. This study employed a new conditional mouse model, Rosa26creERT2/chACE2, which expresses human ACE2 (hACE2) multiple organs, investigate effects of SARS-CoV-2 beyond system. strain demonstrated susceptibility in dose and sex-dependent manner, showing that infected male mice exhibited more severe disease outcomes, significant weight loss, pronounced lung pathology dysfunction, increased mortality, compared females. In contrast intratracheal infection, intranasal virus administration facilitated viral spread brain, thereby underscoring nasal route's role pathogenesis neurological manifestations. Intranasal also led innate immune system activation administration, even though both routes activated adaptive response. model provides valuable tool individual tissues or use multisystemic approach, it advances possibilities for preclinical evaluation antiviral therapies vaccine strategies.

Language: Английский

Engineered exosomes derived from stem cells: a new brain-targeted strategy DOI
Ahmed Waqas,

Songze Huang,

Lukui Chen

et al.

Expert Opinion on Drug Delivery, Journal Year: 2024, Volume and Issue: 21(1), P. 91 - 110

Published: Jan. 2, 2024

Using engineered exosomes produced from stem cells is an experimental therapeutic approach for treating brain diseases. According to reports, preclinical research has demonstrated notable neurogenesis and angiogenesis effects using modified cell-derived exosomes. These biological nanoparticles have a variety of anti-apoptotic, anti-inflammatory, antioxidant properties that make them very promising nervous system disorders.

Language: Английский

Citations

7
New mouse model for inducible hACE2 expression enables to dissect SARS-CoV-2 pathology beyond the respiratory system DOI Creative Commons

Federica Gambini,

Dominik Arbon, Petr Nickl

et al.

Mammalian Genome, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 22, 2025

The Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) infection is not limited to the respiratory tract as receptors, including angiotensin-converting enzyme 2 (ACE2), are expressed across many tissues. This study employed a new conditional mouse model, Rosa26creERT2/chACE2, which expresses human ACE2 (hACE2) multiple organs, investigate effects of SARS-CoV-2 beyond system. strain demonstrated susceptibility in dose and sex-dependent manner, showing that infected male mice exhibited more severe disease outcomes, significant weight loss, pronounced lung pathology dysfunction, increased mortality, compared females. In contrast intratracheal infection, intranasal virus administration facilitated viral spread brain, thereby underscoring nasal route's role pathogenesis neurological manifestations. Intranasal also led innate immune system activation administration, even though both routes activated adaptive response. model provides valuable tool individual tissues or use multisystemic approach, it advances possibilities for preclinical evaluation antiviral therapies vaccine strategies.

Language: Английский

Citations

0