Agricultural science and practice, Journal Year: 2024, Volume and Issue: 10(3), P. 46 - 60
Published: Feb. 28, 2024
Aim. To isolate and identify bacteria with antagonist properties for biocontrol of the agents bacterial diseases soryz (Sorghum oryzoidum) sorghum crops. Methods. The studies were conducted in 2021-2023. Spore-forming isolated from samples, collected fields experimental farm Uman National Horticulture University (Cherkasy region, Uman). Lactic acid plants, private land plot, located between villages Teolyn, Vladyslavchyk, Kniazhyky Monastyryshche com- munity, district, where Pershotravneve hamlet used to be situated. A total 1,250 samples analyzed. experiment had three repeats. lactic surface plants on firm ripe stage summer while isolating phytopathogenic bacteria. isolates bacteria- antagonists also inner part winter stubble stalk soryz, tilled field. antagonistic activity strains spore-forming bacteria, different ecological niches, phytopathogens crops was determined vitro. Pseudomonas syringae, spots, as test-cultures: 211141a, 211141, 210341, 21034, 210521, along collection phytopathogens: syringae 8299, subsp. UKM B-1021, X. oryzae 8375, Dickeya chrysanthemi 8683, Diskeya chryzanthemi 8683. extracted studied using method radial strokes (joint cultivation under investigation). deemed inactive if growth delay zone 0–5 mm (–), 5 10 (+) – low activity, 11–20 (++) moderate over 20 (+++) high regarding test-cultures. check effect isolate-antagonist artificial infecting field conditions. For this purpose, a diurnal culture introduced into concentration 1×108 colony-forming units, 24 h later, test-strain phytopathogen administered above previous puncture. results evaluated 7–14 days after infection. which demonstrated their an- tagonistic identified by morphological properties, Gram staining, catalase test, profile carbohydrate fermentation mass-spectrometry (MALDI-TOF Matrix Assisted Laser Desorption/Ionization) VITEK MS mass-spectrometer. Results. Thirty-eight iso- lates soryz; among these, 21030, 21095, 21040, ASV1, ASV3, B4 towards investigated Isolate 21040 showed most test-strains P. (the negative 23–30 mm) lower cultures. Isolates AVS3 selective phytopatho- gens. Twenty extracted. Higher noted 8/1 F1 highest 210521 8375 40–35 mm). In conditions, treatment affected pathological process that developed due impact led reduction disease symptoms. taxonomic position seem promising control agents, determined. terms morphology cells colonies, biochemical profile, MALDI-TOF, Bacillus subtilis, ASV3 vallismortis. Lactobacillus pentosus sakei 8/1. Conclusions. addition we subtilis B4, vallismortis such Lactiplantibacillus (Latilactobacillus 8/1), elaboration methods diseases.
Language: Английский