The First Report of mcr-1-Carrying Escherichia coli, Isolated from a Clinical Sample in the North-East of Romania DOI Creative Commons

Maria Vlad,

Brîndușa-Elena Lixandru,

Andrei-Alexandru Muntean

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(12), P. 2461 - 2461

Published: Nov. 29, 2024

Colistin resistance poses a significant clinical challenge, particularly in Gram-negative bacteria. This study investigates the occurrence of plasmid-mediated colistin among Enterobacterales isolates (Escherichia coli, Klebsiella pneumoniae, and Enterobacter spp.) non-fermentative rods (Acinetobacter baumannii Pseudomonas aeruginosa). We analyzed 114 colistin-resistant that were selected, based on phenotypes, isolated between 2019 2023. To achieve this, we used rapid immunochromatographic test, NG-Test® MCR-1; multiplex PCR for mcr-1 to mcr-8, real-time mcr-2. One E. coli isolate was identified as carrying gene, confirmed by MCR-1, whole-genome sequencing. strain, belonging ST69, harbored four plasmids, harboring different antimicrobial genes, with being located 33,304 bp circular IncX4 plasmid. No mcr-2 mcr-8-positive detected, prompting further investigation into alternative mechanisms. is first report mcr-1-positive, from human sample North-East Romania.

Language: Английский

Genetic Characteristics of Antimicrobial Resistance in Escherichia coli Isolated from Farm Animals, Slaughterhouses, and Associated Environments DOI Creative Commons
Debby Fadhilah Pazra,

Dinda Iryawati

IntechOpen eBooks, Journal Year: 2025, Volume and Issue: unknown

Published: March 11, 2025

Antimicrobial resistance (AMR) in Escherichia coli from farm animals, slaughterhouses, and associated environments poses a critical threat to public health food safety. Several studies have reported that antibiotic genes (ARGs) are found with high prevalence, especially blaCTX-M, mcr-1, tetA tetB, aac(6′)-Ib, qnr. These were frequently mobile genetic elements (MGEs) such as IncF IncI plasmids, class 1 integrons, insertion sequences (e.g., IS26), transposons, which facilitate horizontal gene transfer adaptation selective pressures. Comparative analysis indicated animals slaughterhouse act reservoirs convergence points for resistant E. strains diverse sources, amplifying the spread of genes. Environmental samples, including wastewater demonstrated overlap isolates suggesting inadequate waste management contributes AMR propagation has potential zoonotic transmission. This chapter will explain characteristics antibiotic-resistant focus on dominant ARGs MGEs drive dissemination resistance. information is needed monitoring evolution so appropriate policies interventions can be implemented prevent control coli.

Language: Английский

Citations

0
Prevalence, antimicrobial resistance, and genetic profile of Escherichia coli in retail chicken parts in Zagazig City, Egypt DOI
Heba A. Ahmed,

A. S. El-tahlawy,

Rasha M. El Bayomi

et al.

International Journal of Food Microbiology, Journal Year: 2025, Volume and Issue: 436, P. 111211 - 111211

Published: April 17, 2025

Language: Английский

Citations

0
One Health at Risk: Plasmid-Mediated Spread of mcr-1 Across Clinical, Agricultural, and Environmental Ecosystems DOI Creative Commons
Abdelaziz Touati, Nasir A. Ibrahim, Assia Mairi

et al.

Antibiotics, Journal Year: 2025, Volume and Issue: 14(5), P. 506 - 506

Published: May 15, 2025

The global dissemination of plasmid-mediated mcr genes, which confer resistance to the last-resort antibiotic colistin, represents a critical public health challenge driven by interplay clinical, agricultural, and environmental factors. This review examines genetic ecological dynamics mcr-bearing plasmids, focusing on their role in disseminating colistin across diverse bacterial hosts ecosystems. Key plasmid families demonstrate distinct evolutionary strategies, including IncI2, IncHI2, IncX4. IncI2 plasmids favor stability livestock clinical settings. IncHI2 other hand, leverage transposons co-select for multidrug resistance, while IncX4 achieve through streamlined, conjugation-efficient architectures. pervasive spread genes is exacerbated integration into chromosomes via mobile elements co-selection with classes, amplifying multidrug-resistant phenotypes. Environmental reservoirs, food chains, anthropogenic practices further facilitate cross-niche transmission, underscoring interconnectedness under One Health framework. Addressing this crisis requires coordinated reducing misuse agriculture, enhancing surveillance high-risk types, fostering international collaboration preserve antimicrobial efficacy mitigate threat untreatable infections.

Language: Английский

Citations

0
Pathogenicity of Multidrug-Resistant Salmonella typhimurium Isolated from Ducks DOI Creative Commons

Yulin Xu,

Zhitong Yu,

Shaopeng Wu

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(7), P. 1359 - 1359

Published: July 2, 2024

(

Citations

2
The Effect of Manure Application Rates on the Vertical Distribution of Antibiotic Resistance Genes in Farmland Soil DOI Creative Commons

Yuqian Wang,

Liqiong Yang,

Weipeng Liu

et al.

Soil Systems, Journal Year: 2024, Volume and Issue: 8(3), P. 89 - 89

Published: Aug. 14, 2024

Manure application is the primary input route for antibiotic resistance genes (ARGs) in farmland soil. This study investigated effects of varying rates five chicken manure applications on accumulation and distribution ARGs across different soil depths (0–20, 20–40, 40–60 cm) using metagenomic sequencing. The results revealed that was closely linked to depth influenced some extent by fertilizer quantity after 30 days fertilization. were predominantly concentrated surface exhibited a significant decrease type abundance with an increased depth. Compared treated chemical fertilizers alone, manure-treated presented higher diversity ARGs. However, did not increase proportionally increasing ratios (0, 25, 50, 75, 100%). primarily conferred host bacteria through efflux pumps (~33%), target alteration (~31%), inactivation (~20%), protection (~8%). Correlation analysis involving microorganisms widespread multidrug among microorganisms. Furthermore, two genera human pathogenic (Pseudomonas sp. Listeria sp.) identified as potential microbial hosts all treatments. environmental factors indicated are heavy metals paper offers valuable insights risk assessments regarding utilization livestock resources. Additionally, it furnishes scientific foundation strategies pertaining manure.

Language: Английский

Citations

1
The First Report of mcr-1-Carrying Escherichia coli, Isolated from a Clinical Sample in the North-East of Romania DOI Creative Commons

Maria Vlad,

Brîndușa-Elena Lixandru,

Andrei-Alexandru Muntean

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(12), P. 2461 - 2461

Published: Nov. 29, 2024

Colistin resistance poses a significant clinical challenge, particularly in Gram-negative bacteria. This study investigates the occurrence of plasmid-mediated colistin among Enterobacterales isolates (Escherichia coli, Klebsiella pneumoniae, and Enterobacter spp.) non-fermentative rods (Acinetobacter baumannii Pseudomonas aeruginosa). We analyzed 114 colistin-resistant that were selected, based on phenotypes, isolated between 2019 2023. To achieve this, we used rapid immunochromatographic test, NG-Test® MCR-1; multiplex PCR for mcr-1 to mcr-8, real-time mcr-2. One E. coli isolate was identified as carrying gene, confirmed by MCR-1, whole-genome sequencing. strain, belonging ST69, harbored four plasmids, harboring different antimicrobial genes, with being located 33,304 bp circular IncX4 plasmid. No mcr-2 mcr-8-positive detected, prompting further investigation into alternative mechanisms. is first report mcr-1-positive, from human sample North-East Romania.

Language: Английский

Citations

0