Diversity of culturable bacterial isolates and their potential as antimicrobial against human pathogens from Afar region, Ethiopia DOI Creative Commons

Sisay Demisie,

Dong‐Chan Oh, Dawit Wolday

et al.

Microbiology Spectrum, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 4, 2024

ABSTRACT Antimicrobial resistance is a growing global concern exacerbated by the scarcity of new medications and to current antibiotics. Microbes from unexplored habitats are promising sources natural products combat this challenge. This study aimed isolate bacteria producing secondary metabolites assess their antimicrobial efficacy against human pathogens. Soil liquid samples were collected Afar region, Ethiopia. Bacterial isolates obtained using standard serial dilution techniques. activity was evaluated agar plug well diffusion methods. matrix-assisted laser desorption/ionization time-of-flight-mass spectrometry (MALDI-TOF MS) whole-genome sequencing (WGS) conducted for exhibiting highest activity. Secondary extracted analyzed gas chromatography-mass spectra (GC-MS). In study, 301 identified, which 68 (22.6%) demonstrated antagonistic at least one reference pathogen. Whole-genome revealed that Sl00103 belongs genus Bacillus, designated as Bacillus sp. Sl00103. The extract showed zones inhibition ranging between 17.17 ± 0.43 26.2 0.4 mm bacterial pathogens 19.5 0.44 21.0 1.01 Candida albicans . GC-MS analysis ethyl acetate n -hexane extracts identified major compounds including (R,R)-butane-2,3-diol; 3-isobutylhexahydropyrrolo[1,2a] pyrazine-1,4-dione; cyclo(L-prolyl-L-valine); tetradecanoic acid, 12-methyl-, methyl ester; hexadecanoic ester among other. conclusion, isolated several strains region in Ethiopia, with strain (Bacillus Sl00103) demonstrating notable antioxidant activities warranting further studies. IMPORTANCE (AMR) an escalating health threat affecting humans, animals, environment, underscoring urgent need alternative pathogen control Natural products, particularly bacteria, continue be vital source However, microbial Africa remain largely unexplored. we screened Ethiopia’s characterized extreme conditions like high temperatures, volcanic activity, salinity, hot springs identify potential bioactive compounds. We discovered diverse various pathogens, Sl00103), significant activities. compounds, highlighting pharmaceutical applications investigation.

Language: Английский

Synergistic impact of Serendipita indica and Zhihengliuella sp. ISTPL4 on the mitigation of arsenic stress in rice DOI Creative Commons
Neha Sharma, Gaurav Yadav, Jaagriti Tyagi

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: May 29, 2024

Arsenic (As) is a highly toxic metal that interferes with plant growth and disrupts various biochemical molecular processes in plants. In this study, the harmful effects of As on rice were mitigated using combined inoculation root endophyte Serendipita indica an actinobacterium Zhihengliuella sp. ISTPL4. A randomized experiment was conducted, which plants grown under controlled conditions As-stressed conditions. The control treatment groups consisted untreated non-stressed (C1), treated (C2), stressed (T1), (T2). Various phenotypic characteristics such as shoot length (SL), (RL), fresh weight (SFW), (RFW), dry (SDW), (RDW) parameters chlorophyll content, protein antioxidant enzymatic activities evaluated. activity enzymes increased T2 followed by T1 Furthermore, high concentrations phytohormones ethylene (ET), gibberellic acid (GA), cytokinin (CK) found at 4.11 μmol mg −1 , 2.53 3.62 FW plant, respectively. results AAS indicated accumulation roots (131.5 kg ) than (120 ). It showed there sequestration microbial-treated (T2) uninoculated (T1). Our data suggest microbial combination can be used to reduce increasing SOD, CAT, PAL, PPO POD. withstand stress owing active synthesis presence combinations.

Language: Английский

Citations

5
Microbes and metabolites of a plant-parasite interaction: Deciphering the ecology of Tetrastigma host choice in the world’s largest parasitic flower, Rafflesia DOI Creative Commons
Jeanmaire Molina, Roche C. de Guzman, Rinat R. Abzalimov

et al.

Current Plant Biology, Journal Year: 2025, Volume and Issue: 42, P. 100456 - 100456

Published: Feb. 4, 2025

Language: Английский

Citations

0
Positive Effect of Serendipita indica on Fenugreek and Its Tolerance Against Cadmium Stress DOI

Fatemeh Sadat Sakhai,

Zahra Movahedi,

Mehdi Ghabooli

et al.

Current Microbiology, Journal Year: 2025, Volume and Issue: 82(4)

Published: March 9, 2025

Language: Английский

Citations

0
Molecular underpinning of heavy metal sequestration through advanced remediation strategies in higher plants DOI Creative Commons
Himani Agarwal, Divya Chaudhary, Himanshi Aggarwal

et al.

Plant Stress, Journal Year: 2025, Volume and Issue: unknown, P. 100881 - 100881

Published: May 1, 2025

Language: Английский

Citations

0
Trifolium repens L. recruits root-associated Microbacterium species to adapt to heavy metal stress in an abandoned Pb-Zn mining area DOI
Xinqi Yuan,

Yi-yi Liu,

Si-chen Wang

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 946, P. 174057 - 174057

Published: June 22, 2024

Language: Английский

Citations

2
Antibacterial, Antioxidant and Anticancer Activities of the Streptomyces PML5 strain isolated from Carbonate rocksin the Amazon DOI Creative Commons
Karine Rodrigues do Nascimento Chaves,

Maria Lucila Texeira de Andrade França,

Anna Ludmylla Oliveira Mendes

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: March 25, 2024

Abstract The aim of this study was to investigate the antimicrobial and cytotoxic potential Streptomyces sp. isolated from carbonate rock in Amazon. activity verified by diffusion technique agar against pathogens such as Micrococcus lutteus ATCC 7468, Streptococcus pneumoniae ATCC700699; Proteus vulgaris 13315, Escherichia coli 10536, Acinetobacter baumanii 19606.Cytotoxic investigated ​​by inhibition cell growth (%) samples tested human colorectal carcinoma line (HCT116), at a concentration 10 µg/mL (mean ± SEM; n = 2). isolate showed broad spectrum test microorganisms. This maximum antibacterial 10536 (13 0.0 mm) 13315 (12.8 0.29 most resistant microorganism 19606 (11.5 1.15 mm). metabolites extracted solid : liquid method with ethyl acetate organic solvent. ​​The percentage crude extract (HCT116) 96,55%. determination antioxidant capturing free radical ABTS had an 35.60 2.01%, while DPPH 10.65 1.95%. LC-MS analysis presence four peaks, where it identified that absorbance substances 5,791 13,556 were similar 9,921 17,077, respectively; suggests possibility PML 5 having metabolic pathway synthesizing same class. CG-MS volatile compounds. main compound Cyclododecane (83.75%) compared other bioactive These results reinforce Amazonian biodiversity, revealing actinobacteria produce interest pharmaceutical industry.

Language: Английский

Citations

1
Significance of secondary metabolites elicited by Zhihengliuella sp. ISTPL4 in plant growth promotion under arsenic stress DOI
Neha Sharma, Gaurav Yadav, Monika Koul

et al.

South African Journal of Botany, Journal Year: 2024, Volume and Issue: 174, P. 383 - 392

Published: Sept. 19, 2024

Language: Английский

Citations

0
Diversity of culturable bacterial isolates and their potential as antimicrobial against human pathogens from Afar region, Ethiopia DOI Creative Commons

Sisay Demisie,

Dong‐Chan Oh, Dawit Wolday

et al.

Microbiology Spectrum, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 4, 2024

ABSTRACT Antimicrobial resistance is a growing global concern exacerbated by the scarcity of new medications and to current antibiotics. Microbes from unexplored habitats are promising sources natural products combat this challenge. This study aimed isolate bacteria producing secondary metabolites assess their antimicrobial efficacy against human pathogens. Soil liquid samples were collected Afar region, Ethiopia. Bacterial isolates obtained using standard serial dilution techniques. activity was evaluated agar plug well diffusion methods. matrix-assisted laser desorption/ionization time-of-flight-mass spectrometry (MALDI-TOF MS) whole-genome sequencing (WGS) conducted for exhibiting highest activity. Secondary extracted analyzed gas chromatography-mass spectra (GC-MS). In study, 301 identified, which 68 (22.6%) demonstrated antagonistic at least one reference pathogen. Whole-genome revealed that Sl00103 belongs genus Bacillus, designated as Bacillus sp. Sl00103. The extract showed zones inhibition ranging between 17.17 ± 0.43 26.2 0.4 mm bacterial pathogens 19.5 0.44 21.0 1.01 Candida albicans . GC-MS analysis ethyl acetate n -hexane extracts identified major compounds including (R,R)-butane-2,3-diol; 3-isobutylhexahydropyrrolo[1,2a] pyrazine-1,4-dione; cyclo(L-prolyl-L-valine); tetradecanoic acid, 12-methyl-, methyl ester; hexadecanoic ester among other. conclusion, isolated several strains region in Ethiopia, with strain (Bacillus Sl00103) demonstrating notable antioxidant activities warranting further studies. IMPORTANCE (AMR) an escalating health threat affecting humans, animals, environment, underscoring urgent need alternative pathogen control Natural products, particularly bacteria, continue be vital source However, microbial Africa remain largely unexplored. we screened Ethiopia’s characterized extreme conditions like high temperatures, volcanic activity, salinity, hot springs identify potential bioactive compounds. We discovered diverse various pathogens, Sl00103), significant activities. compounds, highlighting pharmaceutical applications investigation.

Language: Английский

Citations

0