Evaluation of Faecal Microbiota Following Probiotics in Infants of Mothers with Gestational Diabetes Mellitus Trial: Protocol for Double-Blind Placebo-Controlled Randomized Trial

Microorganisms, Journal Year: 2025, Volume and Issue: 13(1), P. 112 - 112

Published: Jan. 8, 2025

(1) Background: The incidence of gestational diabetes mellitus (GDM) is rising globally. current evidence indicates that GDM, especially in conjunction with maternal overweight, can alter the composition infants' gut microbiota, potentially increasing risk inflammatory diseases, metabolic disorders, and neurodevelopmental issues later life. Probiotic supplantation early life might establish eubiosis mitigate future complications. To best our knowledge, no study has evaluated effects probiotics on dysbiosis infants mothers GDM. (2) Methods: This will be a single-centre, double-blind, randomized, placebo-controlled trial enrolling sixty neonates born after 35 weeks gestation to participants randomly assigned receive either triple-strain probiotic or placebo for four months. primary objective assess effectiveness supplementation correcting GDM at months age. Faecal microbiome shall estimated using 16SrRNA shotgun sequencing. secondary outcomes include quantification faecal short-chain fatty acids birth months, as well growth developmental assessments four, twelve, twenty-four (3) Trial registration: protocol registered (ACTRN12624000930583p) Australian Clinical Trials registry (ANZCTR).

Language: Английский

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Impact of Gut Microbiome Modulating Interventions on Fecal Metabolome of Infants: A Systematic Review and Quality Assessment

PROTEOMICS, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 27, 2025

ABSTRACT The development of the gut microbiome in infancy is a vulnerable process that may be perturbed by antibiotics or supported probiotics. Although effects these “biotics” have been well‐studied through DNA sequencing, it remains unclear how resulting compositional changes affect metabolic functions. Additionally, limits method standardization require careful quality assessment studies reporting fecal metabolome. We conducted systematic search Embase and MEDLINE for describing metabolites from term near‐term infants, together with anti‐, pre‐, probiotic intervention. identified 680 articles, which 60 were assessed eligibility 21 included. first developed operational checklists transparent reproducible evaluated metabolomic methodologies. This analysis our aim to summarize metabolome induced biotic interventions. Despite varying methodology, we similarities profiles response specific Among most frequently observed metabolites, consistently reported altered after interventions, bile acids, aromatic amino short‐chain fatty acids. conclude discussion on appropriate experimental design, controls, metabolomics guide future research permitting meta‐analyses.

Language: Английский

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Identifying biomarkers and trajectories of executive functions and language development in the first 3 years of life: Design, methods, and findings of the Germina cohort study

Development and Psychopathology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 11

Published: March 5, 2025

This paper reports the methods and preliminary findings of Germina, an ongoing cohort study to identify biomarkers trajectories executive functions language development in first 3 years life. 557 mother-infant dyads (mean age mothers 33.7 years, 65.2% white, 48.7% male infants) have undergone baseline are currently collecting data for other timepoints. A linear regression was used predict Bayley-III using scores derived from data-driven sparse partial least squares utilizing a multiple holdout framework 15 domains. Significant associations were found between socioeconomic/demographic characteristics (B = 0.29), epigenetics 0.11), EEG theta 0.14) beta activity microbiome functional pathways 0.08) domains, infant measured by at T1, suggesting potential interventions prevent impairments.

Language: Английский

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Early-life gut microbiome is associated with behavioral disorders in the Rio birth cohort

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 13, 2025

Emerging evidence has been linking changes in the early-life gut microbiome and neurodevelopmental outcomes. The founder bacteria that first colonize infant's determine microbial succession signals host tissues impact development including brain. Here we investigated association between meconium neurobehavior. To this end, surveyed 16S rRNA gene on samples assessed behavioral outcomes at six-months of age by Denver Developmental Screening Test II (DDST-II). Among four domains investigated, personal-social domain was associated with significant differences bacterial beta diversity (unweighted UniFrac; R2 0.078, p = 0.021) reduced alpha (β −2.290, 95% CI −4.212; −0.368), after adjustment for gestational antibiotics, preterm delivery, delivery mode. Besides, altered neurobehavior (failing to meet milestone) overrepresented Ruminococcaceae, Christensenellaceae, Eubacterium, Treponema, Senegalimassilia, Ruminiclostridium, Roseburia, Romboutsia, Prevotella, Veillonella seminalis. Predicted functional genes showed abundance (all q < 0.15). Fine gross motor skills presented no associations microbiome. This pilot study shows deserve further studies different neonate populations.

Language: Английский

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The Role of Microbiome in Nervous and Immune System Development

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

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Identifying biomarkers and trajectories of executive functions and language development in the first 3 years of life: design, methods and initial findings of the Germina cohort study

Published: May 9, 2024

This paper reports the methods and preliminary findings of Germina, an ongoing cohort study to identify biomarkers trajectories executive functions language development in first 3 years life. 557 mother-infant dyads (mean age mothers 33.7 years, 65.2% white, 48.7% male infants) have undergone baseline are currently collecting data for other timepoints. A linear regression was used predict Bayley-III using scores derived from data-driven sparse partial least squares utilizing a multiple holdout framework 15 domains. Significant associations were found between socioeconomic/demographic characteristics (B=0.29), epigenetics (B=0.11), EEG theta (B=0.14) beta activity microbiome functional pathways (B=0.08) domains, infant development, suggesting potential interventions prevent impairments.

Language: Английский

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Role of Human Milk Microbiota in Infant Neurodevelopment: Mechanisms and Clinical Implications

Children, Journal Year: 2024, Volume and Issue: 11(12), P. 1476 - 1476

Published: Nov. 30, 2024

Background: Human milk (HM) is recognized as an ideal source of nutrition for newborns; a result, its multiple bioactive molecules can support the growth healthy newborns and reduce risk mortality diseases such asthma, respiratory infections, diabetes (type 1 2), gastrointestinal disorders ulcerative colitis Crohn’s disease. Furthermore, it severity necrotizing enterocolitis (NEC) in preterm infants. Moreover, human oligosaccharides (HMOs) present breast show immunomodulatory, prebiotic, neurodevelopmental effect that supports microbiota–gut–brain axis. Material methods: This study examined state-of-the-art research, using keywords “breastfeeding”, “human oligosaccharides”, “microbiota–gut–brain axis”, “infants”, “malnutrition”. The literature review was conducted by selecting articles between 2013 2024, most recent ones. databases used were Web Science, PubMed, Scopus. Results: We found studies examining composition HM infant formula (IF). However, further longitudinal randomized control trials (RCTs) are needed to better understand clinical outcomes components exert on hospitalized children how, conditions malnutrition, necessary newborn. Conclusions: In this review, we affirm importance and, through it, modulation microbiota neuroprotective role newborns, determining health following years life.

Language: Английский

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The neuroscience of mental illness: Building toward the future

Cell, Journal Year: 2024, Volume and Issue: 187(21), P. 5858 - 5870

Published: Oct. 1, 2024

Language: Английский

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Association of maternal postpartum depression symptoms with infant neurodevelopment and gut microbiota

Frontiers in Psychiatry, Journal Year: 2024, Volume and Issue: 15

Published: May 21, 2024

Introduction Understanding the mechanisms underlying maternal postpartum depression (PPD) and its effects on offspring development is crucial. However, research association between PPD, gut microbiota, neurodevelopment remains limited. This study aimed to examine of PPD symptoms with early microbiome, metabolome, in infants at 6 months. Methods Maternal were assessed using Edinburgh Postpartum Depression Scale (EPDS) 42 days postpartum. Infants stool samples collected after birth analyzed 16S rRNA sequencing liquid chromatography–mass spectrometry (LC–MS) detection. Infant was measured months Ages Stages Questionnaire, Third Edition (ASQ-3). Correlations metabolites identified through co-occurrence network analysis. Finally, mediation analyses conducted determine potential causal pathways. Results A total 101 mother-infant dyads included final born mothers had lower neurodevelopmental scores These also increased alpha diversity microbiota abundant Veillonella Finegoldia , while depleted abundance Bifidobacterium Dialister Cronobacter Megasphaera. Furthermore, alterations observed metabolite levels linked Alanine, aspartate, glutamate metabolic pathway, primarily characterized by decreases N-Acetyl-L-aspartic acid, L-Aspartic L-Asparagine. Co-occurrence revealed that acid mediated relationship infant problem-solving skills. Conclusions are associated infants. provides new insights into intervention for whose mother experienced PPD. Further warranted elucidate biological these associations.

Language: Английский

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The gut microbiome as a central player in aging: mechanisms and health outcomes

Problems of Geroscience, Journal Year: 2024, Volume and Issue: 3, P. 154 - 160

Published: Sept. 20, 2024

The article explores the mechanisms by which gut microbiome influences human aging and associated diseases. authors examine age-related changes in composition of microbiota their effects on inflammation, immune response, intestinal barrier permeability. Special attention is given to pathogenic interactions between development cardiovascular, metabolic, neurodegenerative role imbalance, known as dysbiosis, highlighted one key accelerating process. review provides a comprehensive analysis recent studies that demonstrate impact microbial metabolites various body systems through «gut-brain,» «gut-cardiovascular,» «gut-endocrine» axes.

Language: Английский

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