Nano-Infrared Detection and Identification of Bacteria at the Single-Cell Level

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 21, 2025

Every year, bacterial infections are responsible for over 7 million deaths globally. Timely detection and identification of these pathogens enable timely administration antimicrobial agents, which can save thousands lives. Most the currently known approaches that address needs time- labor consuming. In this study, we examine potential innovative nano-infrared spectroscopy, also as atomic force microscopy infrared (AFM-IR) machine learning in different bacteria. We demonstrate a single bacteria cell is sufficient to identify Borreliella burgdorferi, Escherichia coli, Mycobacterium smegmatis, two strains Acinetobacter baumannii with 100% accuracy. The based on vibrational bands originate from components wall well interior biomolecules cell. These results indicate nano-IR spectroscopy be used nondestructive, confirmatory, label-free pathogenic microorganisms at single-cell level.

Language: Английский

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Conductive biological materials for in vitro models: properties and sustainability implications

In vitro models, Journal Year: 2025, Volume and Issue: unknown

Published: April 24, 2025

Abstract The integration of conductive biological materials into in vitro models represents a transformative approach to advancing biomedical research while addressing critical sustainability challenges. Traditional used tissue engineering and disease modeling are often environmentally detrimental, derived from non-renewable resources, limited their ability replicate the dynamic properties native tissues. Conductive bridge this gap by offering unique combination biodegradability, sustainability, functional properties, such as bioelectricity biocompatibility, that essential for mimicking physiological environments. Herein, development current applications biodegradable materials, including advanced polymers polyaniline polypyrrole, carbon-based nanocomposites, renewable biopolymers lignin cellulose, overviewed. These not only reduce ecological footprint but also enable precise simulation electrical signaling tissues, cardiac, neural, muscular systems, thereby enhancing relevance models. Their three-dimensional (3D) constructs, organ-on-chip platforms, bioprinting technologies facilitates patient-specific models, paving way personalized therapeutic diagnostic applications. In addition precision, these align with global efforts implement circular economy principles research, promoting resource efficiency waste reduction. By combining environmental responsibility state-of-the-art functionality, redefining future 3D accelerating innovation regenerative medicine, drug development, fostering sustainable framework scientific discovery.

Language: Английский

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Application of Fourier transform infrared spectroscopy (FTIR) for protozoan analysis: A systematic review

Photodiagnosis and Photodynamic Therapy, Journal Year: 2024, Volume and Issue: unknown, P. 104441 - 104441

Published: Dec. 1, 2024

Protozoa present in water for human consumption represent a significant public health risk to greater extent the most vulnerable populations. Identifying protozoa traditional way through microscopy or with more advanced technologies such as molecular biology may limitations sensitivity, specificity, time, and costs. Fourier Transform Infrared (FTIR) spectroscopy have potential an alternative detection of used consumption. An exhaustive search was carried out databases, SCIELO, PubMed, SCOPUS Google Scholar, terms "protozoa," "protozoan," "parasite," "FTIR," "infrared spectroscopy." Only six articles met inclusion criteria. FTIR can detect changes biochemical composition but has not been identification parasites clinical environmental samples. The systematic review identified lack studies this area need conduct research aimed at developing standardized methods creating spectral database banks protozoan species that will allow precise Cryptosporidium spp. Giardia

Language: Английский

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