THE LIVING MEDICINE INSIDE US DOI Creative Commons
K. M. Salim Andalib, Ahsan Habib

Published: Sept. 28, 2024

Large body of empirical evidence have confirmed the intrinsic relationship between human gut microbial metabolism and its host health. These findings not only establish foundation for pioneering bio-therapeutic strategies utilizing microbiota but also propel ongoing pursuit identifying bacteria with potential health benifits.To further contribute into knowledge pool, this study isolated 6 focusing on their health-promoting attributes, from a fecal consortium 30 healthy donors. Further, 16S rRNA gene sequencing identified subsequently, metabolites derived were meticulously scrutinized anti-microbial, anti-oxidant anti-thrombotic activities.Series in vitro experiments reveal significant therapeutic potentials bacteria. Crude all demonstrated robust properties (IC50 values ranging 150 to 350 μg/mL). Furthermore, isolates B1, B2, BHI2 exhibited bacteriostatic effects various clinically pathogenic strains, including E. coli ATCC-8739, S. typhi ATCC-1408, aureus ATCC-6538. Notably, M2-derived showcased superior efficacy lysing blood clots. Additionally, extracts displayed substantial capacity prolong coagulation time, thereby affirming potential.The outcomes preliminary research offer fresh insights metabolic functions bacteria, extending beyond confines gastrointestinal tract. This underscores paramount importance exploring these active prospective clinical applications.

Language: Английский

Gut Microbiota and Their Metabolites as Modulators of Vascular Complications in Diabetes DOI Creative Commons

Meng Yun Duan,

Jielu Wen,

Anning Chen

et al.

Published: Jan. 7, 2025

Review Gut Microbiota and Their Metabolites as Modulators of Vascular Complications in Diabetes Meng Duan 1,2,3,†, Jielu Wen 1,2,†, Anning Chen 1,2,† Sifan 1,2,* 1 Guangdong Provincial Key Laboratory Malignant Tumor Epigenetics Gene Regulation, Guangdong-Hong Kong Joint for RNA Medicine, Medical Research Center, Sun Yat-Sen Memorial Hospital, University, Guangzhou 510000, China 2 Nanhai Translational Innovation Center Precision Immunology, Foshan 528200, 3 Zhongshan School Yat-sen * Correspondence: [email protected] † These authors contributed equally to this work. Received: 8 November 2024; Revised: 29 Accepted: 24 December Published: 7 January 2025 Abstract: With the global rise population aging, along with increasing burden overweight obesity, prevalence diabetes is expected surge dramatically. Microvascular macrovascular complications are leading causes death among patients type diabetes. Recent advancements have provided evidence suggesting that gut microbiota directly or indirectly regulate vascular function. This review focuses on complex interactions between its metabolites In particular, we highlight novel therapeutic effects interventions such probiotics, dietary modifications, fecal transplantation improving composition reducing risk findings not only provide new insights into pathological mechanisms diabetic but also reveal ideas guiding formulation future treatment strategies.

Language: Английский

Citations

0
Quercetin ameliorates obesity and inflammation via microbial metabolite indole-3-propionic acid in high fat diet-induced obese mice DOI Creative Commons
Jiaheng Lu, Yanting Huang, Yujing Zhang

et al.

Frontiers in Nutrition, Journal Year: 2025, Volume and Issue: 12

Published: April 16, 2025

Obesity is a chronic metabolic disease, mainly caused by excessive/abnormal fat accumulation, as well accompanied endotoxemia and inflammation. Quercetin, natural flavonoid, may alleviate obesity regulating gut microbiota metabolites, but its exact mechanism for improving unknown. The aim of this study was to investigate the effects quercetin on high-fat diet (HFD)-induced in mice. In particular, we focused regulatory tryptophan metabolite indole-3-propionic acid (IPA). C57BL/6J mice were subjected 20-week HFD feeding regimen with concurrent daily oral administration or IPA. body weight, barrier function, inflammation determined. Gut composition analyzed 16S rRNA sequencing IPA levels measured serum feces. vitro experiments, Caco-2 cells used evaluate fecal dilutions from quercetin-treated tight junction protein expression aryl hydrocarbon receptor (AhR) activation. Our results revealed that supplementation significantly mitigated inflammation, improved disrupted function through actvation AhR/interleukin 22 (IL-22) pathway. treatment increased abundance Lactobacillus. Quercetin intervention feces alleviated enhanced intestinal AhR findings further corroborated cell experiment, which indicated modulation dysregulated change microbial coordinated improvement effect disruption. alleviates restoring induced disorder, elevates level activate AhR/IL-22 pathway, thereby enhancing integrity suppressing

Language: Английский

Citations

0
Engineering artificial microbial consortia for personalized gut microbiome modulation and disease treatment DOI
Timoth Mkilima

Annals of the New York Academy of Sciences, Journal Year: 2025, Volume and Issue: unknown

Published: May 5, 2025

Abstract The human gut microbiome is a complex ecosystem that plays vital role in maintaining health and contributing to the pathogenesis of various diseases. This review proposes transformative approach involves engineering artificial microbial consortia—precisely designed communities microorganisms—for personalized modulation targeted therapeutic interventions. By integrating synthetic biology, systems advanced culturing techniques, tailored consortia can be developed perform specific functions within gut, including production molecules, immune responses, competition against pathogenic bacteria. In vitro vivo studies indicate these engineered effectively restore balance enhance host resilience. holds immense potential revolutionize healthcare by addressing root causes diseases such as metabolic disorders, inflammatory conditions, gastrointestinal infections through precise manipulation microbiome. Future research should focus on rigorous clinical trials evaluate safety, efficacy, long‐term impacts diverse populations, paving way for innovative therapies promote overall well‐being.

Language: Английский

Citations

0
Potential therapeutic application and mechanism of gut microbiota-derived extracellular vesicles in polycystic ovary syndrome DOI Open Access

Liangliang Yang,

Tingxiu Liu,

Yan Liao

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 180, P. 117504 - 117504

Published: Sept. 27, 2024

Language: Английский

Citations

1
Gut microbiota and kidney diseases. Literature review DOI
Evgeny Shutov, Bolshakov S.A. Bolshakov,

Tatyana A Makarova

et al.

Nephrology and Dialysis, Journal Year: 2024, Volume and Issue: 26(3), P. 283 - 302

Published: Sept. 20, 2024

Recent technological advances have significantly enhanced our understanding of the role microbial communities play in human body. The gut microbiota, one most diverse microbiomes, consists over 35,000 bacterial species and 10 million genes, leading researchers to consider it as an additional organ. This whiles relatively stable within each individual highly influenced by exogenous endogenous factors. Collectively, microbiota functions a "second genome", profoundly impacting host’s metabolic pathways regulating body’s complex homeostatic balance. Research into "microbe–host" interaction, both health disease, has garnered worldwide scientific. In chronic kidney disease (CKD), undergoes significant changes, growing evidences suggests that dysbiosis plays crucial progression renal failure. Key pathological process, such production gut-derived uremic toxins, decreased synthesis short– chain fatty acids, altered intestinal pH, compromised barrier function, heightened systemic inflammation, are all linked microbiota. However, relationship between these changes pathogenesis requires further investigation. Advances microbiome research, including metagenomic metatranscriptomic analyses, alongside proteomic, metabolomics, immunomic studies, greatly expanded microbiomal community structure functions. These technologies, coupled with mechanistic experiments model systems, deepened knowledge how influences metabolism. Current research aims explore bidirectional host, identifying potential interventions could help restore mutualistic relationship.

Language: Английский

Citations

0
THE LIVING MEDICINE INSIDE US DOI Creative Commons
K. M. Salim Andalib, Ahsan Habib

Published: Sept. 28, 2024

Large body of empirical evidence have confirmed the intrinsic relationship between human gut microbial metabolism and its host health. These findings not only establish foundation for pioneering bio-therapeutic strategies utilizing microbiota but also propel ongoing pursuit identifying bacteria with potential health benifits.To further contribute into knowledge pool, this study isolated 6 focusing on their health-promoting attributes, from a fecal consortium 30 healthy donors. Further, 16S rRNA gene sequencing identified subsequently, metabolites derived were meticulously scrutinized anti-microbial, anti-oxidant anti-thrombotic activities.Series in vitro experiments reveal significant therapeutic potentials bacteria. Crude all demonstrated robust properties (IC50 values ranging 150 to 350 μg/mL). Furthermore, isolates B1, B2, BHI2 exhibited bacteriostatic effects various clinically pathogenic strains, including E. coli ATCC-8739, S. typhi ATCC-1408, aureus ATCC-6538. Notably, M2-derived showcased superior efficacy lysing blood clots. Additionally, extracts displayed substantial capacity prolong coagulation time, thereby affirming potential.The outcomes preliminary research offer fresh insights metabolic functions bacteria, extending beyond confines gastrointestinal tract. This underscores paramount importance exploring these active prospective clinical applications.

Language: Английский

Citations

0