Microbial Diversity, Functional Genomics and Antibiotic Resistance in Integrated Chicken and Fish Farming Systems in Bangladesh DOI

S. M. Kador,

Jannatul Ferdus Shila,

Sinthea Afrin

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 18, 2024

Abstract The Integrated Fish Farming (IFF) system, practiced in Bangladesh for its economic benefits and resource efficiency, requires an understanding of microbial diversity, functional genomics, antimicrobial resistance to optimize efficiency sustainability. This study delves into the compositions, antibiotic within diverse environmental samples using 16S rRNA sequencing KEGG pathway analysis. taxonomic analysis revealed a community comprising 2838 OTUs, with Bacteria (99.81%) dominating over Archaea (0.19%). Sediment exhibited highest archaeal primarily consisting Euryarchaeota, Parvarchaeota, Crenarchaeota. Bacterial diversity encompassed 70 phyla, Firmicutes being predominant, particularly chicken gut samples. Notable bacterial genera included Lactobacillus Weissella. Alpha highlighted significant richness sediment fish intestine samples, while beta Bray-Curtis PCoA indicated distinct compositions across sample types. Functional genomic metabolic genes as most predominant all focusing on amino acid, carbohydrate, energy metabolism. Noteworthy pathways ribosome biogenesis ABC transporters, abundant feed Antibiotic susceptibility testing 55 isolates demonstrated high rates, notably against Tetracyclines Fluoroquinolones, Escherichia coli Proteus mirabilis exhibiting resistance. identified through pathways, such bcrC vanX, were indicating profiles. comprehensive profiling underscores complex ecosystems various dominance these environments, concerning levels among common pathogens. These findings emphasize need ongoing surveillance targeted interventions mitigate spread communities. is first country reveal genomics Chicken settings.

Language: Английский

The intestinal microbial community and function of Riptortus pedestris at different developmental stages and its effects on development DOI Creative Commons
Yanbin Wang, Rong Li, Chun‐Jing Wang

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: Jan. 22, 2025

Introduction Riptortus pedestris is a destructive pest that threatens multiple leguminous crops in China. The intestinal microbiota plays crucial role the growth and reproduction of host insects. However, composition function gut at different developmental stages remain unclear. Methods Here, metagenomic sequencing was performed to clarify microbial diversity 2nd-, 3rd-, 4th-, 5th- instar nymphs (2 N–5 N) female adults (FAs) R. effects vital bacteria on development detected. have stage specificity, indicating their . Results Enterococcus Caballerronia were predominant present during 2 N–FAs. In addition, abundances 3 N 4 guts significantly greater than those others guts. Furthermore, 5 harbored abundant Burkholderia - Paraburkholderia Caballeronia metabolic pathways enriched from FAs. Carbohydrate metabolism, including glycoside hydrolases (GHs) glycosyl transferases (GTs), occurs throughout entire stage. Many antibiotic resistance genes (ARGs) detected Pseudomonadota Bacillota presented broad spectrum resistance. Excitingly, eliminated by treatment unable molt normally, lifespan shortened nymphs, had significant effect nymph development. Conclusion summary, our results, for first time, systematically illustrate abundance across demonstrate genera are This study provides basis stinkbug management strategies focus pivotal microbiota.

Language: Английский

Citations

0
The Invisible Threat of Antibiotic Resistance in Food DOI Creative Commons
Gabriella Kiskó,

Belma Bajramović,

Fatma Elzhraa

et al.

Antibiotics, Journal Year: 2025, Volume and Issue: 14(3), P. 250 - 250

Published: March 1, 2025

The continued and improper use of antibiotics has resulted in the emergence antibiotic resistance (AR). dissemination antibiotic-resistant microorganisms occurs via a multitude pathways, including food supply. failure to comply with regulatory withdrawal period associated treatment domestic animals or illicit as growth promoters contributed proliferation bacteria meat dairy products. It was demonstrated that not only do animal human pathogens act donors genes, but also lactic acid can serve reservoirs genes encoding for resistance. Consequently, consumption fermented foods presents potential conduit AR. This review provides an overview transmission range traditional novel foods. literature data reveal foodborne microbes be significant factor

Language: Английский

Citations

0
Microbiome and Microbial Pure Culture Study Reveal Commensal Microorganisms Alleviate Salmonella enterica Serovar Pullorum Infection in Chickens DOI Creative Commons
Jianshen Zhu,

Jinmei Ding,

Kaixuan Yang

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(9), P. 1743 - 1743

Published: Aug. 23, 2024

Pullorum disease, an intestinal disease in chickens caused by Salmonella enterica serovar pullorum (S. Pullorum), is a significant threat to the poultry industry and results substantial economic losses. The bacteria’s transmission, both vertical horizontal, makes it difficult completely eliminate it. Control strategies for primarily involve stringent eradication programs that cull infected birds employ antibiotics treatment. However, are costly, antibiotic use restricted. Therefore, developing alternative control essential. Increasingly, studies focusing on modulating gut microbiota diseases. Modulating chicken may offer novel strategy preventing controlling poultry. impact of S. has not been well established, prompting our exploration relationship between this study. In study, we initially analyzed dynamic distribution with Pullorum. Alpha diversity analysis revealed decrease observed OTUs Shannon index group, suggesting reduction richness due infection. Principal coordinate (PCoA) showed distinct clusters uninfected groups, indicating infection changed structure. Specifically, enriched relative abundance genera Escherichia-Shigella (65% vs. 40.6% groups) Enterococcus (10.8% 3.7%) while reducing Lactobacillus (9.9% 32%) microbiota. Additionally, based changes microbiota, isolated microorganisms, including Bifidobacterium pseudolongum, Streptococcus equi Lacticaseibacillus paracasei (L. paracasei), which were decreased Notably, L. Lp02 strain was found effectively inhibit proliferation vitro alleviate its vivo. We reduced decreasing anaerobic genus Lactobacillus. Furthermore, enabled isolation several antimicrobial microorganisms from healthy feces, notably inhibiting alleviating Overall, research enhances understanding interaction pathogen infection, as offers new perspectives disease.

Language: Английский

Citations

1
Microbial Diversity, Functional Genomics and Antibiotic Resistance in Integrated Chicken and Fish Farming Systems in Bangladesh DOI

S. M. Kador,

Jannatul Ferdus Shila,

Sinthea Afrin

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 18, 2024

Abstract The Integrated Fish Farming (IFF) system, practiced in Bangladesh for its economic benefits and resource efficiency, requires an understanding of microbial diversity, functional genomics, antimicrobial resistance to optimize efficiency sustainability. This study delves into the compositions, antibiotic within diverse environmental samples using 16S rRNA sequencing KEGG pathway analysis. taxonomic analysis revealed a community comprising 2838 OTUs, with Bacteria (99.81%) dominating over Archaea (0.19%). Sediment exhibited highest archaeal primarily consisting Euryarchaeota, Parvarchaeota, Crenarchaeota. Bacterial diversity encompassed 70 phyla, Firmicutes being predominant, particularly chicken gut samples. Notable bacterial genera included Lactobacillus Weissella. Alpha highlighted significant richness sediment fish intestine samples, while beta Bray-Curtis PCoA indicated distinct compositions across sample types. Functional genomic metabolic genes as most predominant all focusing on amino acid, carbohydrate, energy metabolism. Noteworthy pathways ribosome biogenesis ABC transporters, abundant feed Antibiotic susceptibility testing 55 isolates demonstrated high rates, notably against Tetracyclines Fluoroquinolones, Escherichia coli Proteus mirabilis exhibiting resistance. identified through pathways, such bcrC vanX, were indicating profiles. comprehensive profiling underscores complex ecosystems various dominance these environments, concerning levels among common pathogens. These findings emphasize need ongoing surveillance targeted interventions mitigate spread communities. is first country reveal genomics Chicken settings.

Language: Английский

Citations

0