Biofilm Resilience: Molecular Mechanisms Driving Antibiotic Resistance in Clinical Contexts

Biology, Journal Year: 2025, Volume and Issue: 14(2), P. 165 - 165

Published: Feb. 6, 2025

Healthcare-associated infections pose a significant global health challenge, negatively impacting patient outcomes and burdening healthcare systems. A major contributing factor to healthcare-associated is the formation of biofilms, structured microbial communities encased in self-produced extracellular polymeric substance matrix. Biofilms are critical disease etiology antibiotic resistance, complicating treatment infection control efforts. Their inherent resistance mechanisms enable them withstand therapies, leading recurrent increased morbidity. This review explores development biofilms their dual roles disease. It highlights structural protective functions EPS matrix, which shields populations from immune responses antimicrobial agents. Key molecular biofilm including restricted penetration, persister cell dormancy, genetic adaptations, identified as barriers effective management. implicated various clinical contexts, chronic wounds, medical device-associated infections, oral complications, surgical site infections. prevalence hospital environments exacerbates challenges underscores urgent need for innovative preventive therapeutic strategies. evaluates cutting-edge approaches such DNase-mediated disruption, RNAIII-inhibiting peptides, DNABII proteins, bacteriophage nanoparticle-based solutions, coatings, lock therapies. also examines associated with biofilm-related diagnostic difficulties, disinfectant economic implications. emphasizes multidisciplinary approach importance understanding dynamics, role pathogenesis, advancements strategies combat biofilm-associated effectively settings. These insights aim enhance reduce burden diseases.

Language: Английский

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A versatile nanoplatform with excellent biofilm permeability and spatiotemporal ROS regulation for peri-implantitis treatment

Theranostics, Journal Year: 2025, Volume and Issue: 15(8), P. 3490 - 3516

Published: Feb. 24, 2025

Rationale: Dental implant restoration is essential for rehabilitating dentition defects. However, peri-implantitis (PI) seriously threatens the long-term stability of implants. Treating PI requires complete eradication plaque biofilm and meticulous modulation inflammatory responses. Antibacterial photodynamic therapy (aPDT) presents a promising potential in antibacterial realm. Nonetheless, traditional aPDT faces challenges such as inadequate penetration distribution photosensitizers, well lack precise bacteria targeting. Moreover, excessive ROS generated by will aggravate oxidative stress peri-implant tissues, this issue cannot be neglected. Methods: The CuTA-Por@ε-PL nanoplatforms (CPP NPs) were synthesized characterized using dynamic light scattering, transmission electron microscopy, dye probes detail. anti-inflammatory activities CPP NPs evaluated both vitro vivo. vivo therapeutic efficacy was successively analyzed through micro-CT, hematoxylin eosin staining, Masson's immunofluorescence colony formation units (CFU), among other techniques. Results: Porphyrin (Por), CuTA nanozyme with SOD/CAT activities, ε-Polylysine (ε-PL) combined to fabricate via straightforward approach. notable positive charge facilitated penetration, Then, irradiation 660 nm laser triggered burst elimination. After aPDT, scavenged residual modulated host immunity regulating macrophage polarization. As result, CPP-treated groups demonstrated most outstanding performance rat model. Conclusions: Given pathogenesis PI, strategy rationally designed multifunctional NP functions spatiotemporal regulation. It provides potentially novel approach treatment, which may have profound impact on improving prognosis patients advancing field dentistry.

Language: Английский

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Surface modification strategies to reinforce the soft tissue seal at transmucosal region of dental implants

Bioactive Materials, Journal Year: 2024, Volume and Issue: 42, P. 404 - 432

Published: Sept. 10, 2024

Language: Английский

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Predictors of implant failure: A comprehensive analysis of risk factors in oral implant restoration for patients with partial defects of dentition

Biomolecules and Biomedicine, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

Implant failure remains a significant challenge in oral implantology, necessitating deeper understanding of its risk factors to improve treatment outcomes. This study aimed enhance the clinical outcomes implant restoration by investigating contributing patients with partial dentition defects within two years treatment. Additionally, sought develop an early prediction model for failure. A retrospective analysis was conducted on 300 defects, dividing them into groups: failed group and successful group, based occurrence years. General data condition-specific information were compared between groups. Multivariate binary logistic regression used identify influencing factors, while predictive effectiveness assessed using receiver operating characteristic (ROC) curve. The revealed that such as gender, post-implant smoking, hygiene status at second-year follow-up, tooth position, number implants, timing loading, width keratinized mucosa, bone quantity significantly influenced likelihood (P < 0.05). Among these, smoking position identified independent factors. area under curve (AUC) 0.695, indicating low performance. Although determined be factor years, performance limited.

Language: Английский

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Diagnostic and therapeutic strategies in combating implanted medical device-associated bacterial biofilm infections

Folia Microbiologica, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 27, 2025

Language: Английский

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Enhancing Root Canal Disinfection with Er:YAG Laser: A Systematic Review

Dentistry Journal, Journal Year: 2025, Volume and Issue: 13(3), P. 101 - 101

Published: Feb. 26, 2025

Background: The quest for minimally invasive disinfection in endodontics has led to using Erbium:Yttrium-Aluminum-Garnet (Er:YAG) lasers. Conventional approaches may leave bacterial reservoirs complex canal anatomies. Er:YAG’s strong water absorption generates photoacoustic streaming, improving smear layer removal with lower thermal risk than other laser systems. Methods: This systematic review followed PRISMA 2020 guidelines. Database searches (PubMed/MEDLINE, Embase, Scopus, Cochrane Library) identified studies (2015–2025) on Er:YAG laser-assisted root disinfection. Fifteen articles met the inclusion criteria: antibacterial efficacy, biofilm disruption, or removal. Data settings, irrigants, and outcomes were extracted. of bias was assessed a ten-item checklist, based guidelines from Handbook Systematic Reviews Interventions. Results: All found activation significantly improved over conventional ultrasonic methods. Photon-induced streaming (PIPS) shock wave–enhanced emission (SWEEPS) yielded superior reduction, especially apically, enabled sodium hypochlorite concentrations without sacrificing efficacy. Some research indicated reduced post-operative discomfort. However, protocols, parameters, outcome measures varied, limiting direct comparisons emphasizing need more standardized, long-term clinical trials. Conclusions: irrigation appears highly effective disruption removal, supporting deeper irrigant penetration. While findings are promising, further standardized is needed solidify confirm lasers’ benefits.

Language: Английский

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Dysregulation of the Immune System in Advanced Periimplantitis: Systemic Implications and Inflammatory Mechanisms—A Hematological and Immunological Study

Journal of Clinical Medicine, Journal Year: 2025, Volume and Issue: 14(7), P. 2453 - 2453

Published: April 3, 2025

Objectives: This study aimed to assess the systemic and local inflammatory responses in patients with periimplantitis, focusing on key immune markers clinical parameters. The further explores relationship between markers, indices, dysregulation, particularly regarding T-cell exhaustion inflammation. Methods: A cohort of classified into moderate advanced stages, was compared a control group healthy individuals dental implants. Clinical parameters, including plaque index (API), bleeding probing (BoP), pocket depth (PPD), peri-implant sulcus (PSI), were recorded. Hematological, immunological, biochemical analyses performed, focus cell populations (NK cells, T-cells, their PD-1 PD-L1). Results: Patients periimplantitis exhibited significantly higher indices (API, BoP, PSI, PPD) than group, most pronounced differences group. Hematological analysis revealed increased leukocyte neutrophil counts, whereas NK levels reduced. Immunological profiling indicated elevated PD-L1 expression suggesting dysregulation. Furthermore, strong correlations found PPD values marker levels, highlighting Conclusions: findings confirm that dysregulation plays central role progression. association alterations, emphasizes need for multifactorial diagnostic treatment approach. Integrating modulation strategies, assessments, lifestyle modifications, such as improved oral hygiene smoking cessation, could improve disease management reduce recurrence.

Language: Английский

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Sitagliptin regulates the AMPK/NF-κB signaling pathway to alleviate lipopolysaccharide-induced inflammatory responses and promote osteogenic differentiation in rat bone marrow mesenchymal stem cells

Archives of Oral Biology, Journal Year: 2025, Volume and Issue: unknown, P. 106253 - 106253

Published: April 1, 2025

This study aimed to explore the impact of sitagliptin on inflammatory response and osteogenic differentiation in lipopolysaccharide (LPS)-stimulated rat bone marrow mesenchymal stem cells (BMSCs) clarify underlying mechanisms action. In vitro-cultured BMSCs were identified, treated with a range doses, assessed cell counting kit-8 assay quantify viability. The expression proteins genes relevant inflammation osteogenesis was measured using enzyme-linked immunosorbent real-time quantitative polymerase chain reaction techniques. ability analyzed by alkaline phosphatase staining, activity assay, alizarin red s staining. Adenosine monophosphate-activated protein kinase (AMPK)/nuclear factor-kappa B (NF-κB) signaling pathway activation detected through western blotting. High but not low concentrations significantly suppressed cellular Sitagliptin dose-dependently inhibited LPS-induced responses while facilitating their differentiation. It also activated AMPK suppressing NF-κB activity. inhibitor treatment partially reversed these beneficial effects osteogenesis. suppresses promoting modulation AMPK/NF-κB activity, thereby mitigating functional impairment under microenvironmental conditions.

Language: Английский

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Linking peri-implantitis to microbiome changes in affected implants, healthy implants, and saliva: a cross-sectional pilot study

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: April 17, 2025

The rising use of dental implants is accompanied by an expected increase in peri-implant diseases, particularly peri-implantitis (PI), which poses a significant threat to implant success and necessitates thorough understanding its pathogenesis for effective management. To gain deeper insights into the role impact microbiome progression PI, we analyzed 100 samples saliva subgingival biofilm from 40 participants with healthy (HI group) or co-occurrence diagnosed PI-affected (PI using shotgun metagenomic sequencing. We identified most discriminative species distinguishing diseased study groups through log ratios differential ranking analyses. Mogibacterium timidum, Schaalia cardiffensis, Parvimonas micra, Filifactor alocis, Porphyromonas endodontalis, gingivalis Olsenella uli were associated biofilm. In contrast, Neisseria sp oral taxon 014, Haemophilus parainfluenzae, Actinomyces naeslundii, Rothia mucilaginosa aeria more prevalent Functional pathways such as arginine polyamine biosynthesis, including putrescine citrulline showed stronger correlations implants. health was characterized predominance involved purine pyrimidine deoxyribonucleotide de novo glucose glucose-1-phosphate degradation, tetrapyrrole biosynthesis. Our findings reveal that PI-free cavities differ significantly microbial composition functional compared co-occurring implants, closely resemble PI-associated profiles. This pattern extended salivary samples, where biomarkers follow similar trends.

Language: Английский

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Impact of different chemical debridement agents on early cellular responses to Titanium dental implants: a transcriptome-based In Vitro Study on Peri-implant tissue regeneration

Colloids and Surfaces B Biointerfaces, Journal Year: 2025, Volume and Issue: 253, P. 114727 - 114727

Published: April 25, 2025

Language: Английский

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