Comprehensive immunoinformatics and bioinformatics strategies for designing a multi-epitope based vaccine targeting structural proteins of Nipah virus

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: May 13, 2025

Background Nipah virus (NiV) is characterized by recurring outbreaks and causes severe neurological impact, leading to increased mortality rates. Despite the severity of disease, there no proven post-exposure treatment available, emphasizing critical need for development an effective vaccine. Objective This study was aimed at designing a multi-epitope based vaccine candidate on in-silico approach. Methods NiV’s Structural proteins were screened B T-cell epitopes, assessing characteristics like antigenicity, immunogenicity, allergenicity, toxicity. Two constructs (NiV_1 & 2) designed using different adjuvants (Cholera toxin Beta-defensin 3) linkers their predicted 3D structures evaluated interaction with Toll-Like Receptor TLR-3 docking molecular dynamics (MD) simulation studies. Finally, The potential expression construct in Escherichia coli (E. coli.) verified cloning it into PET28a (+) vector immune simulations undertaken. Results identified 30 conserved, antigenic, immunogenic, non-allergenic, non-toxic epitopes broad population coverage. Based stability MD results, NiV_1 considered further analysis. In-silico indicated substantial immunogenic response. Moreover, codon optimization validated expressions E. coli. Conclusion findings indicate that has elicit both cellular humoral responses. Additional vitro vivo investigations are required validate computational observations.

Language: Английский

Pangenome analysis of five representative Tropheryma whipplei strains following multiepitope-based vaccine design via immunoinformatic approaches

Molecular Genetics and Genomics, Journal Year: 2024, Volume and Issue: 299(1)

Published: Oct. 26, 2024

Language: Английский