Fish as biological models: Implications for gut microbiota research in biomedical and aquaculture sciences

Aquaculture International, Journal Year: 2025, Volume and Issue: 33(4)

Published: May 2, 2025

Language: Английский

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Ecological filtering drives rapid spatiotemporal dynamics in fish skin microbiomes

Molecular Ecology, Journal Year: 2024, Volume and Issue: 33(18)

Published: Aug. 19, 2024

Abstract Skin microbiomes provide vital functions, yet knowledge about the drivers and processes structuring their species assemblages is limited—especially for non‐model organisms. In this study, fish skin microbiome was assessed by high throughput sequencing of amplicon sequence variants from metabarcoding V3–V4 regions in 16S rRNA gene on hosts subjected to following experimental manipulations: (i) translocation between fresh brackish water habitats investigate role environment; (ii) treatment with an antibacterial disinfectant reboot community assembly priority effects; (iii) maintained alone or pairs study social environment inter‐host dispersal microbes. The results revealed that harbour a highly dynamic microbial composition distinct bacterioplankton communities ambient water. Microbiome first diverged as effect either freshwater habitat. When individuals were translocated back water, converged towards summary, external environmental conditions individual‐specific factors jointly determined dynamics, whereas had negligible effects. dynamics seemingly non‐affected treatment, pointing resilience disturbance. emphasised inter‐individual variability unexplained variation found many host‐microbiome systems, although mechanistic underpinnings remain be identified.

Language: Английский

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Intestinal microbiota shifts as a marker of thermal stress during extreme heat summer episodes in farmed gilthead sea bream (Sparus aurata)

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 22, 2024

Climate change and its associated extreme events alter a number of physiological processes that also affect the homeostatic relationship host with their microbial communities. The aim this study was to gain more insights on issue, examining effect record breaking-heat summer 2022 gut microbiota farmed gilthead sea bream (Sparus aurata), reared from May August at IATS research infrastructure (Spain´s Mediterranean coast). Fish were fed daily four experimental diets, containing two different lipid levels (16% 14%) with/without commercial emulsifier (0.1%; Volamel Aqua, Nukamel). On 9th, concurrently historical water temperature (30.49 ºC), fish sampled for analysis blood-stress markers water/intestinal microbiota. Gut clearly evidenced increased abundance bacteria Spirochaetota phylum, mainly represented by genus Brevinema. This shift not driven environmental colonization as remained residual in samples increase temperature. Bayesian network functional enrichment analyses suggested high Brevinema exploits negatively enhances condition imbalance intestinal homeostasis, which almost completely reversed use dietary emulsifiers combination low energized diets. phenotype restoration occurred concomitance changes circulating cortisol glucose. Altogether highlights potential heat-stress biomarker, reinforcing value intervention valuable solution mitigate negative impact global warming aquaculture production.

Language: Английский

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Gilthead seabream mucus glycosylation is complex, differs between epithelial sites and carries unusual poly N-acetylhexosamine motifs

Fish & Shellfish Immunology, Journal Year: 2024, Volume and Issue: 153, P. 109864 - 109864

Published: Aug. 30, 2024

Gilthead seabream (Sparus aurata) is a marine finfish of economic importance in aquaculture.Despite its adaptability to varying culture conditions, gilthead can be affected by viral, bacterial or parasitic diseases.The main route entry pathogens through mucosal surfaces.Teleost external and internal surfaces are covered mucus, mainly comprised highly glycosylated proteins called mucins.The mucin glycans regulate pathogen growth, adhesion, virulence inter intra species communication.Here, we characterized the mucus glycosylation, compared it previously described investigated associations with microbiota.214 were identified.The majority found at more than one epithelial surface, but 27, 22 89 O-glycan structures unique skin, gill intestinal sample groups, respectively.Six core types observed.The skin O-glycans neutral unusual poly HexNAc motifs.In contrast, acidic not 'poly HexNAc' type observed gill.Furthermore, mucosa had less oligomannose complex N-glycans intestine.The concentration diversity bacteria was similar intestine, differed between epithelia co-varied glycan epitopes.The presence glycosylation plenty epitopes for foraging, suggest that defense includes an abundant resident microbiota.This large library provides platform further studies, example aiming identifying use diagnostic purposes, study host-microbe interactions disease intervention therapies.

Language: Английский

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Behavioural Monitoring Underlines Habituation to Repeated Stressor Stimuli in Farmed Gilthead Sea Bream (Sparus aurata) Reared at a High Stocking Density

Biology, Journal Year: 2024, Volume and Issue: 13(11), P. 879 - 879

Published: Oct. 29, 2024

A confinement stress test with 75% tank space reduction and behavioural monitoring through tri-axial accelerometers externally attached to the operculum was designed. This procedure validated by demonstrating less pronounced response in gilthead sea bream than European bass (950-1200 g). Our study aimed assess habituation high stocking densities such bream. Animals (420-450 g) were reared (June-August) a flow-through system at two (CTRL: 10-15 kg/m

Language: Английский

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Moving Beyond Oxford Nanopore Standard Procedures: New Insights from Water and Multiple Fish Microbiomes

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12603 - 12603

Published: Nov. 23, 2024

Oxford Nanopore Technology (ONT) allows for the rapid profiling of aquaculture microbiomes. However, not all experimental and downstream methodological possibilities have been benchmarked. Here, we aimed to offer novel insights into use different library preparation methods (standard-RAP native barcoding-LIG), primers (V3-V4, V1-V3, V1-V9), basecalling models (fast-FAST, high-HAC, super-accuracy-SUP) implemented in ONT elucidate microbiota associated with aquatic environment farmed fish, including faeces, skin, intestinal mucus. Microbial DNA from water faeces samples could be amplified regardless library-primer strategy, but only LIG V1-V3/V1-V9 case skin intestine Low taxonomic assignment levels were favoured by full-length V1-V9 primers, though silico hybridisation revealed a lower number potential matching sequences SILVA database, especially evident increase Actinobacteriota real datasets. SUP execution allowed higher median Phred quality (24) than FAST (11) HAC (17), its time (6-8 h) was comparison other (0.6-7 h). Altogether, optimised water- fish-related microbial analyses, validating, first time, strategy. We consider that LIG-V1-V9-HAC is optimal time/cost-effective option amplify environmental samples. V1-V3 help maximise dataset microbiome diversity, representing an alternative when long amplicon become compromised and/or high host loads interfere PCR amplification/sequencing procedures, gut

Language: Английский

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Intestinal microbiota shifts by dietary intervention during extreme heat summer episodes in farmed gilthead sea bream (Sparus aurata)

Aquaculture Reports, Journal Year: 2024, Volume and Issue: 40, P. 102566 - 102566

Published: Dec. 20, 2024

Language: Английский

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