First report on isolation and characterization of Bacillus Sp. associated with honey bee brood disease DOI Creative Commons
Amin Salehi, Adil Khan, Nasreen Nasreen

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 29, 2025

Bacterial infections in honey bee brood pose a significant threat to populations, leading decreased production and disrupting critical crop pollination networks. While Paenibacillus larvae Melissococcus plutonius are well-established as major pathogens of eggs larvae, the presence other harmful bacteria Khyber Pakhtunkhwa (KPK) province Pakistan remains largely unexplored. Molecular characterization Bacillus species B. cereus isolates revealed key virulence genes, including cry gene, known for its insecticidal properties. The ability produce cytotoxins, haemolysins, enterotoxins raises concerns about impact on larval immunity survival. Similarly, mycoides, close relative cereus, was identified diseased broods, highlighting potential involvement microbial dysbiosis within hive. Reports from North America Europe have also linked these declining health, emphasizing need further investigation into their pathogenic mechanisms. This study investigated bacterial contamination broods collected infected beehives across various cities KPK. Biochemical molecular analyses widespread species, emerging most dominant, followed by cereus. Phylogenetic analysis indicated evolutionary relationship between shared spore-forming characteristics. research is first report infecting shedding light previously unrecognized apiculture region.

Language: Английский

Captive-rearing changes the gut microbiota of the bumblebee Bombus lantschouensis native to China DOI Creative Commons
Feng Zhou,

Shuning Sun,

Xinxin Song

et al.

PeerJ, Journal Year: 2025, Volume and Issue: 13, P. e18964 - e18964

Published: Feb. 13, 2025

Bumblebees play crucial roles as pollinators in both natural agricultural and ecological systems. Their health overall fitness are significantly affected by the gut microbiota, which can maintain intestinal homeostasis hosts regulating their nutritional metabolism. However, information about diversity of microbiota related functional changes during artificial rearing native species is currently limited. This study investigated dynamic remodeling Chinese bumblebee Bombus lantschouensis under captive rearing, supported 16S rRNA amplicon sequencing bacterial DNA. The typical microbial community composition was detected wild B. , with genus Gilliamella Snodgrassella identified dominant strains. Conversely, captive-reared group showed increased decreased abundance certain microorganisms. populations Bifidobacterium Saccharibacter Lactobacillus including Firm-4 Firm-5, were dramatically after captive-rearing became bacteria, while strikingly reduced. Notably, this found that pathogenic bacteria appeared intestines wild-caught disappeared when host reared conditions. shows bumblebees facilitates physiological metabolism commercial insects.

Language: Английский

Citations

0
Influence of Different Diets on Growth and Development of Eastern Honey Bee (Apis cerana) DOI Creative Commons

Ruonan Liang,

Cheng Liang, Yi Zhang

et al.

Insects, Journal Year: 2025, Volume and Issue: 16(4), P. 383 - 383

Published: April 3, 2025

In recent years, honey bees have been stressed by multiple factors, with malnutrition posing a significant threat to the healthy development of bee colonies. To keep colony and productive, beekeepers supply their colonies supplementary pollen or commercial substitutes during periods dearth insufficient quantity quality. this study, we evaluated effects four natural types (oilseed rape pollen, camellia lotus buckwheat pollen) two (Diet 1 Diet 2) against control group (sucrose solution) on Apis cerana through cage experiments. The food consumption, live body weight, longevity, hypopharyngeal gland midgut proteolytic enzyme activity caged workers were measured. consumption rates oilseed greater than other diets. Oilseed recognized as excellent-quality diets for activity. Over entire experimental period, fed had similar diet weight those substitutes, these significantly higher survival rate results indicated that appeared be less beneficial A. resources.

Language: Английский

Citations

0
First report on isolation and characterization of Bacillus Sp. associated with honey bee brood disease DOI Creative Commons
Amin Salehi, Adil Khan, Nasreen Nasreen

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 29, 2025

Bacterial infections in honey bee brood pose a significant threat to populations, leading decreased production and disrupting critical crop pollination networks. While Paenibacillus larvae Melissococcus plutonius are well-established as major pathogens of eggs larvae, the presence other harmful bacteria Khyber Pakhtunkhwa (KPK) province Pakistan remains largely unexplored. Molecular characterization Bacillus species B. cereus isolates revealed key virulence genes, including cry gene, known for its insecticidal properties. The ability produce cytotoxins, haemolysins, enterotoxins raises concerns about impact on larval immunity survival. Similarly, mycoides, close relative cereus, was identified diseased broods, highlighting potential involvement microbial dysbiosis within hive. Reports from North America Europe have also linked these declining health, emphasizing need further investigation into their pathogenic mechanisms. This study investigated bacterial contamination broods collected infected beehives across various cities KPK. Biochemical molecular analyses widespread species, emerging most dominant, followed by cereus. Phylogenetic analysis indicated evolutionary relationship between shared spore-forming characteristics. research is first report infecting shedding light previously unrecognized apiculture region.

Language: Английский

Citations

0