The transcription factor YbdO attenuates the pathogenicity of avian pathogenic Escherichia coli by regulating oxidative stress response DOI Creative Commons
Lumin Yu, Yuzhong Zhao, Shanpeng Zhang

et al.

BMC Microbiology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Dec. 28, 2024

Avian pathogenic Escherichia coli (APEC) is a significant pathogen infecting poultry that responsible for high mortality, morbidity and severe economic losses to the industry globally, posing substantial risk health of poultry. APEC encounters reactive oxygen species (ROS) during infection process thus has evolved antioxidant defense mechanisms protect against oxidative damage. The imbalance ROS production defenses known as stress, which results in damage proteins, lipids DNA, even bacterial cell death. uses transcription factors (TFs) handle stress. While many TFs E. have been well characterized, mechanism YbdO TF on protecting regulating virulence pathogenicity not clarified. Here we focus regulatory APEC. from this study showed attenuated chicks models by inhibiting expression genes fepG ycgV using quantitative real-time reverse PCR (RT-qPCR) experiments. electrophoretic mobility shift assays (EMSA) confirmed specifically bound promoters ycgV. Additionally, increases H2O2-induced via repressing stress response sodA, soxR, ahpC, ahpF, katG, oxyR binding their promoter regions. repression effect facilitates host immune eliminate generate beneficial protection body, thereby indirectly attenuating These findings might provide further insights into offer new perspectives studies prevention control infections.

Language: Английский

The Linc009 negatively regulates cell apoptosis in turbot (Scophthalmus maximus L.) during bacterial challenge DOI Creative Commons

Beibei Wang,

Xiaocheng Zhu, Xiaohua Zhuang

et al.

Comparative Immunology Reports, Journal Year: 2025, Volume and Issue: unknown, P. 200211 - 200211

Published: Feb. 1, 2025

Language: Английский

Citations

0
The transcription factor YbdO attenuates the pathogenicity of avian pathogenic Escherichia coli by regulating oxidative stress response DOI Creative Commons
Lumin Yu, Yuzhong Zhao, Shanpeng Zhang

et al.

BMC Microbiology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Dec. 28, 2024

Avian pathogenic Escherichia coli (APEC) is a significant pathogen infecting poultry that responsible for high mortality, morbidity and severe economic losses to the industry globally, posing substantial risk health of poultry. APEC encounters reactive oxygen species (ROS) during infection process thus has evolved antioxidant defense mechanisms protect against oxidative damage. The imbalance ROS production defenses known as stress, which results in damage proteins, lipids DNA, even bacterial cell death. uses transcription factors (TFs) handle stress. While many TFs E. have been well characterized, mechanism YbdO TF on protecting regulating virulence pathogenicity not clarified. Here we focus regulatory APEC. from this study showed attenuated chicks models by inhibiting expression genes fepG ycgV using quantitative real-time reverse PCR (RT-qPCR) experiments. electrophoretic mobility shift assays (EMSA) confirmed specifically bound promoters ycgV. Additionally, increases H2O2-induced via repressing stress response sodA, soxR, ahpC, ahpF, katG, oxyR binding their promoter regions. repression effect facilitates host immune eliminate generate beneficial protection body, thereby indirectly attenuating These findings might provide further insights into offer new perspectives studies prevention control infections.

Language: Английский

Citations

0