International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(24), P. 13330 - 13330
Published: Dec. 12, 2024
Submerged plants can thrive entirely underwater, playing a crucial role in maintaining water quality, supporting aquatic organisms, and enhancing sediment stability. However, they face multiple challenges, including reduced light availability, fluctuating conditions, limited nutrient access. Despite these stresses, submerged demonstrate remarkable resilience through physiological biochemical adaptations. Additionally, their interactions with microbial communities are increasingly recognized as pivotal mitigating environmental stresses. Understanding the diversity of is for comprehending complex between environments. This research aims to identify screen microbes from plant samples capable producing 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase explore metagenomic analysis. Microbes were isolated screened ACC production, techniques, co-occurrence network analysis, used examine within communities. deaminase-producing significantly enhance metabolism under stress conditions. The identification culturable bacteria revealed that most belong genera Pseudomonas, Bacillus, Acinetobacter. A total 177 strains cultured, molecular revealing 79 reductant, 86 non-reductant, 12 uncultured strains. Among 162 activity, 50 tested positive. To further understand dynamics, collected both natural sources artificial pond reservoirs assess impact location on flood-associated microbiomes plants. Metagenomic analysis was conducted epiphytic endophytic samples. By exploring overall composition dynamics associated plants, this seeks deepen our understanding plant–microbe screening helped diverse activity amplicon sequencing paved way towards identifying shaping microbiome microbes. Co-occurrence highlighted intricate Notably, observed plant-associated across different locations, distinct variations populations identified
Language: Английский